Mycoplasma hyopneumoniae culture medium and preparation method thereof

Abstract

The invention discloses a mycoplasma hyopneumoniae culture medium and a preparation method thereof, which belong to the technical field of veterinary biological products. Each 1000 ml of phosphate buffer contains 20-50 g of PPLO (pleuropneumonia-like organism), 2-10 g of yeast powder, 3-15 g of glucose, 0.5-4 ml of 1% phenol red, 2-10 g of amino acid composition, 15-30 g of compound traditional Chinese medicine polysaccharide and 50-100 ml of pig serum. When culturing the mycoplasma hyopneumoniae, the mycoplasma hyopneumoniae culture medium disclosed by the invention can improve the tolerance of the mycoplasma hyopneumoniae and reduce the apoptosis rate of the mycoplasma hyopneumoniae; the mycoplasma hyopneumoniae culture medium disclosed by the invention can increase the amount of culture bacteria of the mycoplasma hyopneumoniae, the concentration of the culture bacteria liquid reaches 10<10-11> / ml, which is increased by more than 5-10 times compared with that of a common culture medium; the mycoplasma hyopneumoniae culture medium disclosed by the invention can inhibit the growth of other bacteria when culturing the mycoplasma hyopneumoniae so as to reduce the culture pollution risk of culturing the mycoplasma hyopneumoniae.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a culture medium for mycoplasma hyopneumoniae and a preparation method thereof. Background technique [0002] Mycoplasma swine pneumonia is a very common respiratory disease in pig farms. It is prevalent all over the world. It is commonly known as swine asthma. It is caused by Mycoplasma hyopneumoniae (Mhp). The pathogen mainly resides in the trachea, bronchi and bronchioles in pigs On the cilia of the pig's respiratory tract, it severely damages the physical defense mechanism of the pig's respiratory tract - the defense mucosa, which increases the susceptibility of the pig herd to other respiratory pathogens, such as swine influenza virus, porcine reproductive and respiratory syndrome virus, type Ⅱ porcine circovirus, bronchial Bordetella septica, Actinobacillus pleuropneumoniae and Haemophilus parasuis, etc., often cause complex infections of ...

AI Technical Summary

Problems solved by technology

Although Mycoplasma hyopneumoniae can use the nutrients in the culture medium to synthesize the substances needed for its own growth and reproduction, due to the small molecular weight of its genome, the amount of information it carries is not much, and its biosynthetic ability is limited. Therefore, it needs to ingest cholesterol, amino acids, Fatty acids, nucleic acid precursors, vitamins and other substances, which make the medium composition of mycoplasma more complicated, and some special nutrients must be added

[0004] Because Mycoplasma hyopneumoniae has higher nutritional requirements than ordinary Mycoplasma, the composition of the medium is more complex, the culture time is long, and the culture concentration is low, which greatly affects people's further research on it, and also hinders the development and production of Mycoplasma hyopneumoniae vaccine.

At present, many scholars have designed a variety of culture media for its growth. Although they have their own characteristics, they have not solved the problem of difficult cultivation in general.

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