Mycoplasma hyopneumoniae culture medium and preparation method thereof

A technology of mycoplasma hyopneumoniae and culture medium, which is applied in the field of mycoplasma hyopneumoniae culture medium and its preparation, which can solve the problems of high nutritional requirements, small molecular weight, long culture time, etc., reduce the risk of pollution, inhibit bacterial growth, and improve tolerance Effect

Active Publication Date: 2014-02-05
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Mycoplasma hyopneumoniae can use the nutrients in the culture medium to synthesize the substances needed for its own growth and reproduction, due to the small molecular weight of its genome, the amount of information it carries is not much, and its biosynthetic ability is limited. Therefore, it needs to ingest cholesterol, amino acids, Fatty acids, nucleic acid precursors, vitamins and other substances, which make the medium composition of mycoplasma more complicated, and some special nutrients must be added
[0004] Bec...

Method used

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  • Mycoplasma hyopneumoniae culture medium and preparation method thereof

Examples

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Effect test

Embodiment 1

[0055] A kind of mycoplasma hyopneumoniae culture medium, this culture medium contains the following components in weight ratio in every 1000ml phosphate buffer:

[0056] PPLO 20g

[0057] Yeast powder 6g

[0058] Glucose 8g

[0059] 1% phenol red 4ml

[0060] Amino Acid Combination 4g

[0061] Compound Traditional Chinese Medicine Polysaccharide 15g

[0062] Pig Serum 50ml

[0063] A preparation method for mycoplasma hyopneumoniae culture medium, the steps are:

[0064] (1) Preparation of phosphate buffer solution: Accurately weigh 8g of sodium chloride, 0.2g of potassium chloride, 1.15g of disodium hydrogen phosphate, 0.2g of potassium dihydrogen phosphate, 0.1g of calcium chloride, and magnesium chloride containing 6 crystal water Dissolve 0.1g in 1000ml double distilled water, pass through a 0.22μm filter membrane under aseptic conditions, filter and sterilize for later use.

[0065] (2) Preparation of compound traditional Chinese medicine polysaccharides

[0066] ...

Embodiment 2

[0074] A kind of mycoplasma hyopneumoniae culture medium, this culture medium contains the following components in weight ratio in every 1000ml phosphate buffer:

[0075] PPLO 35g

[0076] Yeast powder 2g

[0077] Glucose 15g

[0078] 1% phenol red 0.5ml

[0079] Amino Acid Combination 10g

[0080] Compound Traditional Chinese Medicine Polysaccharide 30g

[0081] Pig serum 80ml.

[0082] A preparation method of a mycoplasma hyopneumoniae culture medium, the steps of which are the same as in Example 1. The compound traditional Chinese medicine polysaccharide is extracted from 10 parts of Coptis chinensis, 20 parts of Ginkgo biloba, 15 parts of Achyranthes bidentata, 20 parts of Codonopsis pilosula, 50 parts of Poria cocos, 20 parts of wolfberry, 15 parts of licorice and 5 parts of salvia miltiorrhiza.

Embodiment 3

[0084] A kind of mycoplasma hyopneumoniae culture medium, this culture medium contains the following components in weight ratio in every 1000ml phosphate buffer:

[0085] PPLO 50g

[0086] Yeast powder 10g

[0087] Glucose 3g

[0088] 1% phenol red 2.5ml

[0089] Amino Acid Combination 2g

[0090] Compound Traditional Chinese Medicine Polysaccharide 25g

[0091] Pig serum 100ml.

[0092] A preparation method of a mycoplasma hyopneumoniae culture medium, the steps of which are the same as in Example 1. The compound traditional Chinese medicine polysaccharide is extracted from 5 parts of Coptis chinensis, 10 parts of Ginkgo biloba, 20 parts of Achyranthes bidentata, 30 parts of Codonopsis pilosula, 5 parts of Poria cocos, 30 parts of wolfberry, 20 parts of licorice and 10 parts of salvia miltiorrhiza.

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Abstract

The invention discloses a mycoplasma hyopneumoniae culture medium and a preparation method thereof, which belong to the technical field of veterinary biological products. Each 1000 ml of phosphate buffer contains 20-50 g of PPLO (pleuropneumonia-like organism), 2-10 g of yeast powder, 3-15 g of glucose, 0.5-4 ml of 1% phenol red, 2-10 g of amino acid composition, 15-30 g of compound traditional Chinese medicine polysaccharide and 50-100 ml of pig serum. When culturing the mycoplasma hyopneumoniae, the mycoplasma hyopneumoniae culture medium disclosed by the invention can improve the tolerance of the mycoplasma hyopneumoniae and reduce the apoptosis rate of the mycoplasma hyopneumoniae; the mycoplasma hyopneumoniae culture medium disclosed by the invention can increase the amount of culture bacteria of the mycoplasma hyopneumoniae, the concentration of the culture bacteria liquid reaches 10<10-11>/ml, which is increased by more than 5-10 times compared with that of a common culture medium; the mycoplasma hyopneumoniae culture medium disclosed by the invention can inhibit the growth of other bacteria when culturing the mycoplasma hyopneumoniae so as to reduce the culture pollution risk of culturing the mycoplasma hyopneumoniae.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a culture medium for mycoplasma hyopneumoniae and a preparation method thereof. Background technique [0002] Mycoplasma swine pneumonia is a very common respiratory disease in pig farms. It is prevalent all over the world. It is commonly known as swine asthma. It is caused by Mycoplasma hyopneumoniae (Mhp). The pathogen mainly resides in the trachea, bronchi and bronchioles in pigs On the cilia of the pig's respiratory tract, it severely damages the physical defense mechanism of the pig's respiratory tract - the defense mucosa, which increases the susceptibility of the pig herd to other respiratory pathogens, such as swine influenza virus, porcine reproductive and respiratory syndrome virus, type Ⅱ porcine circovirus, bronchial Bordetella septica, Actinobacillus pleuropneumoniae and Haemophilus parasuis, etc., often cause complex infections of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/35
Inventor 张秀文李阳闫艳丽刘雪王二先盛璐丝
Owner 浙江美保龙生物技术有限公司
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