Method for producing biodiesel oil and soil regulator using plant waste

A technology of plant waste and soil conditioner, applied in the field of biodiesel and soil conditioner production, can solve the problems of no patents and no reports, and achieve the effects of improving soil physical and chemical properties, improving soil structure, and loosening soil

Inactive Publication Date: 2007-08-01
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the cost problem limits the industrial production of microbial diesel. Microalgae are low-level plants, and their genetic engineering technology is far less mature than yeast and bacteria. The technical difficulties in high-density cultivation of microalgae are also very obvious.
[0009] If the use of microorganisms to convert waste essential cellulose resources can reduce costs, it is also conducive to environmental protection and sustainable social and economic deve...

Method used

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  • Method for producing biodiesel oil and soil regulator using plant waste

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Embodiment 1

[0031] The corn stalks were properly crushed, added with nutrients, cultured with Trichoderma reesei ATCC46480 at 25℃ for 3 days, added with Rhodotorula glutinis ATCC10659, cultivated for 3 days, harvested, and extracted with organic solvents Oils and fats undergo an exchange reaction with methanol under acid-base catalysis to produce fatty acid methyl esters (ie biodiesel). The solid waste of product production is inoculated with plant endophytes or probiotics (such as the probiotic Trichoderma koningii ATCC201709) Incubate at 20-25°C for 3 days. After mixing, the microbial content and nutrient content in the product are uniform, and it becomes a soil regulator, which is used to improve the soil organic matter, nitrogen fertilizer level, and increase the number of soil microorganisms.

Embodiment 2

[0033] After the rice straw is properly crushed, the straw is directly hydrolyzed with microbial cellulase, hemicellulase, lignin hydrolase, etc., and the hydrolyzate is sterilized by adding nutrients and inoculated with high oil-yielding Rhodotorula glutinosa ATCC10659, fermented for 3 days The yeast cells are harvested, the oil is extracted with an organic solvent, and the exchange reaction with methanol is carried out under acid-base catalysis to produce fatty acid methyl esters (ie, biodiesel). Enzymatically hydrolyzed solid wastes of straw were inoculated with plant endophytes (Phomopsis sp ATCC201398), cultivated at 25°C for 3 days, and the dried mycelium after yeast extraction was blended to become a soil regulator and used to improve soil organic matter , Nitrogen fertilizer level, increase the number of soil microorganisms.

Embodiment 3

[0035]The deciduous leaves or branches of the poplar are crushed and inoculated with plant endophytes that produce high cellulase, lignin hydrolase, and high oil production, such as Chaetomium thermophile ATCC58420 and Phomopsissp. Microorganisms such as ATCC201398, Pestalotiopsis sp (ATCC90291), solid fermentation for 10 days or liquid aeration and stirring fermentation for 5 days, organic solvent extracts oil, and exchange reaction with methanol under acid-base catalysis to produce fatty acid methyl esters (ie biodiesel) ). The solid waste was again inoculated with Phomatophora ATCC201398, Chaetomium ATCC58420 and Polychaeteia ATCC90291 and other strains, cultivated at 25°C for 3 days, and after blending, it became a soil regulator, used to improve soil organic matter, nitrogen fertilizer levels, and increase soil Number of microorganisms.

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Abstract

The invention relates to agricultural energy field, which in detail relates to a method for producing biodiesel and soil modifier with plant waste. The method comprises following steps: disintegrating plant waste, hydrolyzing with normal enzyme or acid and getting hydrolysate, fermentating with hydrolysate to culture bacteria producing fat; inoculating endotrophic bacteria or probiotics to hydrolytic waste; extracting fat from cultured bacteria, methyl esterification for extracted fat and getting biodiesel; mixing bacteria waste and straw hydrolytic waste inculated with endotrophic bacteria or probiotics and getting soil modifier. The invention maximizes efficent use of capital by making use of straw.

Description

Technical field [0001] The invention relates to the field of agricultural energy, in particular to a method for producing biodiesel and soil conditioner by using plant waste. Background technique [0002] The remaining part of wheat, rice, corn, potato, oilseeds, cotton, sugarcane and other crops after harvesting seeds is collectively called straw, and about half of the products of photosynthesis are present in the straw. For example, producing one kilogram of rice can produce 1.5 kilograms of rice straw, one kilogram of wheat can produce 1.5 kilograms of wheat straw, and 1 kilogram of corn can produce 4 kilograms of corn stalk. According to statistics from the United Nations Environment Programme (UNEP), various crops planted in the world produce 1.7 billion tons of straw each year. In recent years, with the development of my country's rural economy and the increase of farmers' income, the energy consumption structure of rural residents is undergoing significant changes, and com...

Claims

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Application Information

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IPC IPC(8): C10G3/00C09K17/14
CPCY02E50/13Y02E50/10Y02P30/20
Inventor 戴传超陶杰邵蔚蓝
Owner NANJING NORMAL UNIVERSITY
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