Poultry and livestock intestinal ecological restoration microbial agent with virus and pathogenic bacteria-resisting functions and antibiotic-replacing effect and preparation method thereof

An ecological restoration and anti-bacteria technology, applied in the field of microbial fermentation, can solve problems that threaten human life and health, soil and water pollution, pig herd death, etc., and achieve the prevention of common respiratory and intestinal diseases with small dosage and significant economic benefits Effect

Inactive Publication Date: 2016-10-12
道拓百旗(天津)房地产信息咨询有限责任公司
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Problems solved by technology

Moreover, in the face of viral colds and viral intestinal diseases, except for individual cases that can be prevented by vaccination, they are almost helpless in the face of constantly mutating viral infections. They can only look at larg...

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  • Poultry and livestock intestinal ecological restoration microbial agent with virus and pathogenic bacteria-resisting functions and antibiotic-replacing effect and preparation method thereof

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preparation example Construction

[0030] A preparation method of an antiviral and antibacterial antibacterial agent for poultry intestinal ecological restoration that replaces antibiotics, inoculates various bacterial strains in corresponding sterilized medium for activation, and prepares first-level seed liquid, and second- and third-level use Based on the principle of similarity and symbiosis, 2 strains of yeast, 3 strains of bacteria, and 2 strains of lactic acid bacteria are mixed and expanded for cultivation. After three stages of expansion, the bacteria liquid is mixed and adsorbed on sterilized wheat bran-rice bran, and then undergoes solid fermentation, and finally low temperature Dry to obtain solid bacterial agent. Among them, the secondary and tertiary mixed expansion culture solutions of two strains of lactic acid bacteria contain water extracts of lemon and astragalus. Specific steps are as follows:

[0031] 1) After activating each slant bacterial classification stored in the refrigerator, the f...

Embodiment 1

[0052] An antiviral and antibacterial antibacterial agent for intestinal ecological restoration of poultry and livestock that replaces antibiotics, including Saccharomyces cerevisiae ACCC20037, Candida utilis ACCC20060, Bacillus subtilis ACCC10114, and Bacillus licheniformis (Bacillus licheniformis) ACCC10146, Rhodopseudomonas palustris ACCC10649, Lactobacillus plantarum ACCC10141 and Lactobacillus rhamnosus ACCC10534. It also contains water extracts of lemon and astragalus (the main component is polysaccharide). All strains were purchased from the China Agricultural Microbiology Culture Collection Center (ACCC), and they are also probiotic strains allowed to be used in feed additives by the Ministry of Agriculture. Non-toxic, harmless, no side effects.

[0053] The preparation method of the poultry and livestock intestinal ecological restoration bacterial agent is to inoculate each bacterial species in the corresponding sterilized medium for activation to prepare a first-lev...

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Abstract

The invention discloses a poultry and livestock intestinal ecological restoration microbial agent with virus and pathogenic bacteria-resisting functions and antibiotic-replacing effect and a preparation method thereof. The poultry and livestock intestinal ecological restoration microbial agent is prepared by mixing saccharomyces cerevisiae, candida utilis, bacillus subtilis, bacillus licheniformis, rhodopseudomonas palustris, lactobacillus plantarum, and lactobacillus rhamnosus. The preparation method comprises the following steps of respectively inoculating each strain into a corresponding sterilization culture medium, activating, and preparing a first-level seed liquid; according to the neighboring and mutualism principle, during second-level and third-level fermenting, mixing and fermenting the saccharomyces cerevisiae and candida utilis, mixing and fermenting the bacillus subtilis and bacillus licheniformis, and mixing and fermenting the rhodopseudomonas palustris, lactobacillus plantarum and lactobacillus rhamnosus; mixing the three mixed and enlarged culture bacteria liquids according to the equal volume of 1:1:1, absorbing onto sterilizing wheat bran-rice bran, and performing solid fermenting and low-temperature drying, so as to obtain the solid microbial agent. The poultry and livestock intestinal ecological restoration microbial agent has the characteristics that the good properties of high density and high activity are realized; the probiotics microbial agent and the lemon and radix astragali seu hedysari water extracting liquid are compounded for first time; the usage amount is less, and the use method is simple and convenient.

Description

Technical field [0001] The invention belongs to microbial fermentation technology, in particular to an antiviral and antibacterial antibacterial agent for ecological restoration of poultry intestinal tract instead of antibiotics and a preparation method thereof. Background technique [0002] For a long time, a large number of or even abused antibiotics in the poultry breeding industry not only made pathogenic microorganisms resistant, but also seriously damaged the ecological balance of poultry intestinal flora, resulting in immunosuppression, low immunity, and disease resistance in poultry and livestock. The capacity is weakened, so viral and bacterial infections increase. The multiple outbreaks of avian influenza in spring and autumn each year are proof. Moreover, in the face of viral colds and viral intestinal diseases, except for individual cases that can be prevented by vaccination, they are almost helpless in the face of constantly mutating viral infections. They can ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/18A61K35/74A61K35/742A61K35/747A61K36/064A61P31/00A23K10/18C12R1/865C12R1/72C12R1/125C12R1/10C12R1/01C12R1/25C12R1/225
CPCC12N1/20A61K35/74A61K35/742A61K35/747A61K36/064C12N1/16C12N1/18A61K2300/00
Inventor 张清敏
Owner 道拓百旗(天津)房地产信息咨询有限责任公司
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