Method for producing ubiquinone by using sticky red rhodotorula

A technology of Rhodotorula viscosum and yeast powder, which is applied in the field of microorganisms to achieve high potential effects

Inactive Publication Date: 2010-01-06
BEIJING UNIV OF CHEM TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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And using the bacteria to produce coenzyme Q 10 No report

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  • Method for producing ubiquinone by using sticky red rhodotorula

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Embodiment 1

[0034] Screening Coenzyme Q 10 High-yielding strain

[0035] The screened strains were purchased from the Institute of Microbiology, Chinese Academy of Sciences, with the number As2,102. For screening Coenzyme Q 10 For high-yielding strains, we adopt the method of activating Rhodotorula viscosus strains first, and after 36 hours of activation, mark lines on the plates that have been poured into the activation medium, pick out the single colonies with the best growth potential, and put them into the enrichment medium The liquid was cultured at 28°C for 36 hours, and then transferred to the solid medium of the enrichment medium for culture. Because the glucose content in this medium was relatively high, the colonies were plump in shape and relatively red in color. Then pick a single colony with the best growth and insert it into the harsh medium, and then insert it into the solid medium lacking nutrients for cultivation. Since the content of nutrients such as nitrogen source a...

Embodiment 2

[0058] Screening Coenzyme Q 10 High-yielding strain

[0059] The screened strains were purchased from the Institute of Microbiology, Chinese Academy of Sciences, and the number in the institute is As2,102. 10 For high-yield strains, the method we adopt is to first activate the Rhodotorula viscosus strain, and after 40 hours of activation, draw lines on the plates that have been poured into the activation medium, pick out the single colonies with the best growth, and put them into the enrichment medium The liquid was cultured at 32°C for 40 hours, and then transferred to the solid culture medium of the enrichment medium. Since the glucose content in this medium was relatively high, the colonies were plump in shape and relatively red in color. Then pick a single colony with the best growth and insert it into the harsh medium, and then insert it into the solid medium lacking nutrients for cultivation. Since the content of nutrients such as nitrogen source and carbon source in th...

Embodiment 3

[0082] Screening Coenzyme Q 10 High-yielding strain

[0083] The screened strains were purchased from the Institute of Microbiology, Chinese Academy of Sciences, and the number in the institute is As2,102. 10 For high-yield strains, the method we adopt is to activate the Rhodotorula viscosus strain first, and after 32 hours of activation, mark the plates on the plates that have been poured into the activation medium, select the single colonies with the best growth, and put them into the enrichment medium The liquid was cultured at 28°C for 32 hours, and then transferred to the solid culture medium of the enrichment medium for culture. Because the glucose content in this medium is relatively high, the colony shape is plump and the color is relatively red. Then pick a single colony with the best growth and insert it into the harsh medium, and then insert it into the solid medium lacking nutrients for cultivation. Since the content of nutrients such as nitrogen source and carbon...

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Abstract

The invention relates to a method for producing coenzyme Q10 by using rhodotorula gracilis, which belongs to the field of microorganism. The method comprises the following steps: culturing bacteria on a flat plate; preserving the bacteria at minus 80 DEG C after being washed by sterile water; and putting the activated bacteria into a fermentation culture medium to culture. The invention provides new high-potential candidate bacteria for fermenting and producing coenzyme Q10.

Description

technical field [0001] The invention relates to a kind of rhodotorula viscosus for producing coenzyme Q 10 The method belongs to the field of microbiology. Background technique [0002] coenzyme Q 10 (Ubiquinone-10, Coenzyme Q 10 , CoQ 10 ) is also known as ubiquinone. Its molecular formula is C 59 h 90 o 4 , with a relative molecular weight of 863.4, is a fat-soluble quinone compound with a chemical name of 2,3-dimethoxy-5-methyl-6-decyl isopentenylbenzoquinone. coenzyme Q 10 It is yellow or orange-yellow crystalline powder at room temperature, with a melting point of 49°C, odorless and tasteless. Because of its long pentadiene side chain, it is easily soluble in chloroform, benzene, carbon tetrachloride, soluble in acetone, petroleum ether and its ether, insoluble in water and methanol. It is easy to decompose into red substance when it sees light, and it is relatively stable to temperature and humidity. The structural formula is as follows: [0003] [0004...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/66C12R1/645
Inventor 袁其朋申晓林陈长京孙新晓
Owner BEIJING UNIV OF CHEM TECH
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