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Tissue engineering skin containing blood vessel structure and its construction method

A tissue-engineered skin and vascular structure technology, applied in skin transplantation, medical science, prosthesis, etc., can solve problems such as defective product cost in mature development cycle and imperfect tissue engineering products.

Inactive Publication Date: 2005-07-13
陕西艾尔肤组织工程有限公司
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  • Abstract
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Problems solved by technology

[0006] Although tissue engineering methods have made great progress in the development of skin, and some products have been applied clinically, the tissue engineering products that have been or are under development are still not perfect, in terms of method maturity, development cycle and product cost. There are defects in many aspects

Method used

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  • Tissue engineering skin containing blood vessel structure and its construction method
  • Tissue engineering skin containing blood vessel structure and its construction method
  • Tissue engineering skin containing blood vessel structure and its construction method

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Embodiment Construction

[0027] The method and implementation effect of the present invention will now be further explained in combination with experiments.

[0028] 1. Cultivation of vascular endothelial cells

[0029] Take the newborn's umbilical cord and place it in sterile phosphate buffered saline PBS, store and transport at 4°C. Try to start the culture within 1 hour, otherwise the cell viability will be greatly reduced; the umbilical cord is placed in a sterile petri dish and buffered with sterile PBS Flush the venous cavity repeatedly to remove dirt and flush the venous cavity to remove blood stains; tightly ligate one end of the umbilical cord vein with sutures, infuse it with digestive juice a at 37°C for 10 minutes, and then digest with digestive juice b for 5 minutes; Cut the ligated umbilical cord from one end, drain the liquid out, then repeatedly wash the blood vessel lumen with PBS, collect the liquid, centrifuge, and wash twice; resuspend the cells with vascular endothelial cell culture m...

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Abstract

The invention relates to a tissue engineered skin containing blood vessel structure and a construction method thereof. It is characterized in that the tissue engineered skin contains skin fibroblasts, keratinocytes and vascular endothelial cells. The construction method of tissue engineered skin comprises the separation of the above three kinds of cells, the culture method and the method of constructing an artificial skin containing a vascular structure, which is characterized in culturing keratinocytes, fibroblasts and human umbilical vein endothelial cells of the skin in vitro, and then using The tissue engineering method mixes these three kinds of cells in proportion in vitro to construct artificial skin with blood vessels. The graft survival rate and the anti-infection ability of tissue engineered skin can be improved, and the application range of tissue engineered skin can be further expanded.

Description

Technical field [0001] The invention belongs to the technical field of constructing artificial organs by tissue engineering methods in biomedical engineering, and relates to a tissue engineering skin containing a blood vessel structure and a construction method thereof. Background technique [0002] In recent years, with the development of tissue engineering technology, reports on the research and development of biological substitutes with the function of repairing, maintaining or improving damaged tissues have gradually increased, and there have been many research reports on artificial skin substitutes. At present, the research and development of tissue engineered skin has become a hot spot for skin defect transplantation treatment. [0003] Skin is an important tissue and organ that covers and protects the surface of the body. It often causes skin defects and abnormalities due to inflammation, ulcers, trauma, burns, tumor surgery, and congenital malformations. For this kind of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61F2/10A61L27/00A61L27/60
Inventor 金岩刘源赵宇
Owner 陕西艾尔肤组织工程有限公司
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