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A kind of hydrogel material for 3D bioprinting and its preparation method and application

A bioprinting and 3D printing technology, applied in additive processing, prosthesis, medical science and other directions, to achieve the effect of small immune rejection, good industrialization prospects, and rapid gel formation

Active Publication Date: 2019-08-02
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing hydrogel systems are difficult to meet many requirements of 3D bioprinting at the same time, such as: rapid gelation under physiological conditions, cell compatibility, mechanical strength, permeability, biodegradability, etc.

Method used

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  • A kind of hydrogel material for 3D bioprinting and its preparation method and application
  • A kind of hydrogel material for 3D bioprinting and its preparation method and application
  • A kind of hydrogel material for 3D bioprinting and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A hydrogel material for 3D bioprinting, consisting of gellan gum, polyethylene glycol acrylate (PEGDA), solvent, and bioactive components. In this example, the bioactive component uses bone morphogenetic protein BMP-2 , its preparation method is as follows:

[0026] (1) Utilize acryloyl chloride to modify PEG end groups by conventional methods to obtain polyethylene glycol acrylate;

[0027] (2) According to the mass fraction, dissolve 0.75% gellan gum, 10% PEGDA, and 0.1% initiator I-2959 in the PBS solution at 80°C, stir evenly, and then add bone morphogenetic protein BMP at 37°C -2, mixing evenly to obtain a hydrogel material;

[0028] (3) 3D printing: mix the above hydrogel material with precursor osteoblast suspension evenly, and the cell density is 5×10 5 cells / mL, placed in the ink tube of the 3D bioprinter, printed into a cell-containing scaffold with a certain structure, after printing, put the material in a UV reaction instrument for 10 minutes, the waveleng...

Embodiment 2

[0031] A hydrogel material for 3D bioprinting, which consists of mercaptolated gellan gum, alkynylated polyethylene glycol, solvent, and bioactive components. In this example, the bioactive component uses the small molecule drug TWS119 (TWS119 is A small-molecule drug that has a significant effect on directionally inducing neural stem cells to differentiate into neurons), the preparation method of which is as follows:

[0032] (1) Utilizing cysteine ​​to modify gellan gum by conventional methods to obtain thiolated gellan gum;

[0033] (2) Utilizing propargyl bromide to modify the PEG end group by conventional methods to obtain alkynylated polyethylene glycol;

[0034] (3) By mass fraction, 0.6% mercaptolated gellan gum, 10% alkynylated polyethylene glycol, and 0.1% initiator I-2959 were dissolved in deionized water at 80° C., stirred evenly, and then Add nanoparticles containing small molecule drug TWS119 (0.5% of TWS119 dosage) at 37°C, mix well to obtain a hydrogel materia...

Embodiment 3

[0038] A hydrogel material for 3D bioprinting, consisting of thiolated gellan gum, polyethylene glycol, solvents, and bioactive components. In this embodiment, the bioactive component uses basic fibroblast growth factor (bFGF) , its preparation method is as follows:

[0039] (1) Utilizing cysteine ​​to modify gellan gum by conventional methods to obtain thiolated gellan gum;

[0040] (2) According to the mass fraction, 1.5% gellan gum and 1% PEG-6000 were dissolved in deionized water at 80°C, stirred evenly, and then 100ng / mL basic fibroblast growth factor was added at 37°C ( bFGF), mixed uniformly to obtain a hydrogel material;

[0041](3) 3D printing: add bone marrow mesenchymal stem cell suspension to the above hydrogel material, and the cell density is 1×10 5 cells / 100μL, placed in the ink tube of the 3D bioprinter, printed a certain structure of the cell-containing scaffold, and immediately placed the material in the bone marrow mesenchymal stem cell culture medium afte...

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Abstract

The invention belongs to the technical field of biological materials, and discloses a hydrogel material for 3D bioprinting and a preparation method and application of the hydrogel material. The hydrogel material comprises the following components in percentages by mass: 0.5-10% of gellan gum and / or derivatives, 0.1-20% of PEG and / or derivatives thereof, 0-1% of a crosslinking initiator, 0-15% of biologically active components and the balance of a solvent. The hydrogel material is double-network hydrogel based on the gellan gum and PEG; interpenetrating double-network structures are formed in a physiological environment; and the hydrogel material has good structure and dimensional stability, and has the advantages of quick gum formation under physiological conditions, excellent cell compatibility, small immunological rejection, high cell encapsulation efficiency, controllable mechanical strength, biodegradability and the like. The hydrogel material is applied to 3D bioprinting; the shortcomings of low curing speed, harsh curing conditions, limited mechanical properties, poor cell compatibility and the like are overcome; and the hydrogel material has obvious advantages and good industrialized prospect.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, in particular to a hydrogel material for 3D bioprinting and its preparation method and application. Background technique [0002] 3D bioprinting is a new type of tissue engineering technology developed on the basis of the development of rapid prototyping technology, combined with the research results of cell biology, computer-aided design and biomaterials. Its ultimate goal is to realize Organ prints. 3D bioprinting technology can overcome the limitations of traditional tissue engineering technology. By precisely positioning the position between biomaterials, biochemical molecules and living cell layers, it can realize the three-dimensional precise positioning of seed cells with different densities in different scaffold materials, and control the functional The spatial location of components, used to fabricate 3D structures. [0003] Hydrogel has high water content, good biocompatibility, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/52A61L27/26A61L27/20A61L27/18A61L27/54A61L27/38B33Y70/00
CPCA61L27/18A61L27/20A61L27/26A61L27/38A61L27/3834A61L27/52A61L27/54A61L2300/216A61L2300/252A61L2300/414B33Y70/00C08L5/00C08L71/02
Inventor 鲁路汪争光吴东蔚周长忍
Owner JINAN UNIVERSITY
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