30 results about "Pancreas islet cell" patented technology

The invention relates to a biscuit with the function of curing the diabetes and a preparation method thereof. The preparation method of the biscuit comprises the steps of: taking fingered citron, Chinese yam, mulberry, medlar, semen coicis, sealwort, fructus citri and kudzu root as raw materials; mixing the components of the raw materials; decocting extracting solution by means of water adding, and respectively filtering by a sieve with 160-180 meshes; concentrating filter liquor to obtain extractum; modulating flourdough according to the weight ratio of the extractum to the flour at 1:8-15; and preparing the biscuit by an existing normal biscuit preparation process. After the biscuit is eaten, the pancreas islet cell can be nourished, the pancreas islet function can be improved, the glucogenic index of the food can be effectively controlled, the fasting blood-glucose and the postmeal glucose can be reduced, the use level of the drug for curing the diabetes can be reduced, and the life quality can be improved.

The invention belongs to the technical field of medical biology, and particularly relates to a preparation method and an application of a scoparia dulcis extract for resisting islet cell apoptosis. According to the preparation method, the scoparia dulcis extract is prepared through the steps of heating reflux, extraction, silica gel column chromatography and the like. Cell experiments and animal experiments prove that the scoparia dulcis extract prepared by the preparation method disclosed by the invention has a remarkable anti-islet cell apoptosis effect and has a bright application prospectin the aspect of preparing medicines for resisting islet cell apoptosis.

The invention belongs to the field of biochemical medicines, and discloses two labdane diterpenoids, and an extraction method and application thereof. The structures of the two labdane diterpenoids are shown in the specification. The labdane diterpenoids are extracted and separated from scoparia dulcis l. Cell experiments prove that the labdane diterpenoids can remarkably resist pancreas islet cell apoptosis.

The present invention relates to an artificial pancreas islet tissue and particularly comprises a biocompatible stent, islet cells and a mixed culture solution. Through a 3D bioprinting technique, thebiocompatible stent and the pancreas islet tissue are prepared into the biologically active artificial pancreas islet tissue. The mixed culture solution is used for culture and preservation of the pancreas islet cells and tissue. The biocompatible stent is prepared using the 3D printing technique. The artificial pancreas islet tissue can be used as a pathophysiological mechanism research and a therapeutic tool for diabetes.

The invention discloses a composite traditional Chinese medicine dropping pill for treating type-II diabetes. The composite traditional Chinese medicine dropping pill comprises the following components in parts by weight: 1-3 parts of coptis chinensis powder, 2-3 parts of rhizoma anemarrhenae powder, 1-2 parts of pseudo-ginseng powder, 0.01-0.03 part of papaverine, 0.02-0.04 part of aminophyllineand 1-3 parts of folium mori. The composite traditional Chinese medicine dropping pill for treating type-II diabetes, which is provided by the invention, comprises main body components, namely finelyselected pure traditional Chinese medicines coptis chinensis, rhizoma anemarrhenae, folium mori and pseudo-ginseng, the formula is reasonable, the components are matched mutually with a synergic function, blood vessel walls can be softened, blood vessels can be expanded, thrombocyte agglomeration is remarkably inhibited, blood viscosity is remarkably reduced, the blood amount is increased, pancreas islet cell blood supply is ensured, normal functions of pancreas islet cells are brought into play, pancreas islet of glucose is balanced, then symptoms of type-II diabetes is relieved, and thus patients are finally relieved from drug dependence. In addition, the composite traditional Chinese medicine dropping pill is capable of effectively relieving hypertension symptoms and is free of side effect on organs of human bodies, and clinical tests show that the effective rate of the dropping pill is greater than 85%, and the dropping pill has the potential of being a good medicine for treating patients suffering from type-II diabetes.

The invention belongs to the technical field of cell differentiation, and particularly relates to a method for differentiating peripheral blood stem cells into endoderm cells and application of the method. According to the invention, peripheral blood stem cells are subjected to induced culture in the presence of BM, Activin A, Wnt3a, TGF-b, LIF, TNF-a, IFN-a, vinterferron-alpha, beta, gamma, B-27 and N2, such that endoderm cells are obtained. The technology is a technical supplement for differentiating the endoderm cells into the islet cells, and the clinical application range of differentiating the stem cells into the islet cells and applying the stem cells to islet transplantation is widened. The technology is expected to solve the islet cell source problem and the islet transplantation heterologous problem, and the clinical effect of islet transplantation and the long-term survival of the graft are improved.

The embodiment of the invention relates to application of a GP73 inhibitor in preparation of medicine for treating diabetes. According to the embodiment of the invention, the inventor finds that GP73 plays a key role in blood glucose regulation, and particularly finds that soluble GP73 can be specifically combined with glucagon to form a compound, the blood glucose increasing function and the gluconeogenesis function of the glucagon are enhanced, and the half-life period of the glucagon is prolonged; the condition that soluble GP73 can activate generation of liver and/or kidney sugar and a gluconeogenesis signal channel in a manner of not depending on glucagon is found; and based on the found regulation effect of GP73 on blood glucose, the inventor also proves that the GP73 inhibitor can reduce the blood glucose level and glycosylated hemoglobin level of diabetic mice, has a protection effect on pancreas islet beta cells, and achieve an effect of treating diabetes through animal experiments.

The invention discloses a double-cell co-packaged microgel for pancreas islet transplantation and a preparation method thereof. The preparation method of the double-cell co-packaged microgel comprises the following steps: respectively synthesizing carbon-carbon double bond modified polymer materials; the preparation method comprises the following steps: dispersing pancreas islets and mesenchymal stem cells into a polymer precursor solution, preparing microgel by using a microfluidic technology, and initiating free aggregation and realizing internal crosslinking of the microgel through blue light, so as to prepare the microgel encapsulating the two cells. The microgel has good biocompatibility and can be used for entrapment of islet cells, the co-encapsulated mesenchymal stem cells can release immunoregulatory factors and inhibit immune cells, and a good immune tolerance environment is provided for cell transplantation.

The invention belongs to the technical field of medicine, and relates to application of synthase FMO3 of intestinal flora metabolite TMAO as a target in screening of drugs or models for preventing and treating pancreas islet function damage. Specifically, the invention discloses application of FMO3 as a target in screening of drugs or biological agents for preventing and treating diseases caused by pancreas islet function impairment and application of FMO3 in preparation of drugs or biological agent models for screening of diseases caused by pancreas islet function impairment. According to the invention, FMO3 is taken as a target and is applied to screening of drugs for promoting glucose-stimulated insulin secretion. The product of FMO3 is TMAO, and a large number of tests prove that TMAO obviously inhibits insulin secretion stimulated by mouse pancreatic beta cell Min6 glucose, knocks down diabetic mouse FMO3, inhibits the level of TMAO, and can improve insufficiency of insulin secretion.

The invention provides islet cells and a separation method thereof, and the separation method comprises the following steps: putting an isolated pancreatic tissue into a phosphate buffer salt solution, and pouring a collagenase solution into the treated pancreatic tissue at multiple parts, so that the pancreatic tissue is fully expanded to obtain a swollen pancreatic tissue; the method comprises the following steps: putting swollen pancreatic tissues into a collagenase-containing solution, centrifuging and digesting for multiple times, adding a buffer solution to terminate digestion, centrifuging, discarding supernatant, and resuspending to obtain a resuspension solution; and filtering, re-suspending the filtrate for multiple times to obtain a minced digestion end product, filtering to obtain a precipitate, blowing the precipitate into a culture dish, and collecting. When collagenase pancreas perfusion is carried out, multi-part slow injection is adopted, so that pancreatic tissues can be fully filled, the operation time is shortened, the operation is simple and convenient, the success rate is high, and pancreas islet injury caused by the fact that a collagenase solution is difficult to reach the interiors of tissue blocks and the digestion speeds inside and outside the tissues are inconsistent in the conventional chopping method is avoided; and the requirements of in-vitro basic experiments can be met.

The invention discloses an induction method for promoting conversion of islet alpha cells into beta cells and application thereof, and the induction method is characterized in that on the basis of a traditional induction method, on the 35th day of induced differentiation, a culture medium for converting the islet alpha cells into the beta cells is replaced, and meanwhile artemether and a GABAA activator NS11394 are added for induction until the 40th day. Through verification, the induction method disclosed by the invention can be used for remarkably improving the expression quantity of the pancreatic beta cells, and is suitable for large-scale production and clinical application of cell preparations.

The invention relates to the field of biological medicines, and in particular, to applications of adiponectin modification to enable islet cells to have antioxidant and/or anti-inflammatory characteristics and applications of adiponectin modification of the islet cells in improvement or enhancement of islet transplantation. The islet cells are subjected to adiponectin modification before islet transplantation, so that the islet cells overexpress adiponectin. The islet cells have the characteristics of oxidation resistance and inflammation resistance. Therefore, islet transplantation results are improved. During gene therapy, the islet cells are decomposed into single cells, so that the infection efficiency of viruses is greatly improved. After the gene therapy, the single cells are re-cultivated into functional cell clusters, so that pancreas islet functions are greatly reserved.

The invention relates to the fields of nano magnetic materials and biology, and discloses a preparation method of degradable immunomagnetic beads. Comprising the following steps: preparing a degradable magnetic bead microsphere raw material, preparing alginate magnetic bead microspheres by adopting a rapid membrane emulsification technology and the degradable magnetic bead microsphere raw material prepared in the step S1, and coupling the alginate magnetic bead microspheres with an antibody; the invention also discloses a degradable immunomagnetic bead. The invention further discloses application of the degradable immunomagnetic beads in products for separating human islet cell clusters. The immunomagnetic bead prepared by the invention can be specifically targeted to the pancreas islet in human pancreas, can realize separation of pancreas islet cell mass and exocrine tissue, can greatly shorten the pancreas islet separation time, improve the separation efficiency, improve the activity and function of the pancreas islet cell mass, improve the transplantation effect, and indirectly reduce the cost of pancreas islet transplantation; the prepared immunomagnetic beads are low in toxicity, good in magnetic response and good in surface biocompatibility, and can be degraded under the condition that the pH value of a solution is high.