Degradable immunomagnetic bead and preparation method thereof

A technology of immunomagnetic beads and magnetic beads, applied in the direction of anti-animal/human immunoglobulin, animal cells, vertebrate cells, etc., can solve the problem that patients are not easy to obtain long-term insulin detachment state, and the density difference between exocrine tissue and pancreatic islet tissue is very small. , the problem of long-term survival of the graft, etc., to achieve the effect of good surface biocompatibility, low toxicity, and reducing the amount of pancreatic islets

Pending Publication Date: 2022-07-05
ZHEJIANG SHUREN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the following problems and confusions exist in the field of islet transplantation: (1) There is a shortage of donors, and the number of islets required for transplantation is large (>11000IEQ / kg body weight); (2) It is difficult for the graft to survive for a long time in the host, and it is difficult for the patient to obtain long-term insulin Out of state; (3) Some problems of immunosuppression, etc.
At present, the conventional clinical islet cell cluster isolation process takes a long time, about 5-8 hours, and requires 4-5 people to operate at the same time. The result of islet isolation is often affected by factors such as the length of hospital stay of the patient, organ harvesting method, storage time and storage conditions. The influence of the influence of the exocrine tissue and the islet tissue often leads to a small density difference, and it is difficult to separate the islet and the exocrine gland tissue by the density gradient method.

Method used

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  • Degradable immunomagnetic bead and preparation method thereof
  • Degradable immunomagnetic bead and preparation method thereof
  • Degradable immunomagnetic bead and preparation method thereof

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Embodiment 1

[0023] The present embodiment provides a method for preparing degradable immunomagnetic beads, comprising the following steps:

[0024] S1: Preparation of degradable magnetic beads and microspheres

[0025] Take 0.05-0.5g sodium alginate, add distilled water to 10mL, dissolve at room temperature for 0.5-1h under magnetic stirring to obtain a 0.5-5% sodium alginate solution; take 5-10mL of the above-prepared sodium alginate solution, add 2-50μL of magnetic beads with a concentration of 50mg / mL and a particle size of 100-500nm, shake up and down to mix the magnetic beads and sodium alginate evenly, as the water phase; in addition, take 100-1000mL of liquid paraffin, add it to the beaker, Then add 0.5-5 mL of Span 80 to the beaker, mix it evenly, and use it as the oil phase; then mix the water phase and the oil phase, stir evenly with a glass rod, and simply disperse the oil phase in the water phase to obtain the raw material of degradable magnetic beads and microspheres ;

[0...

Embodiment 2

[0033] The formula of the degradable immunomagnetic beads of this embodiment is as follows:

[0034]

[0035] The preparation method of the present embodiment is as follows:

[0036] S1: Weigh 0.2 g of sodium alginate, add distilled water to 10 ml, dissolve at room temperature for 0.5-1 h under magnetic stirring, and dissolve evenly to obtain 2% sodium alginate solution; take 10 ml of 2% sodium alginate solution, add 10 μL of the concentration to 50mg / mL magnetic beads with a particle size of 300nm, shake up and down to mix the magnetic beads and sodium alginate evenly, as the water phase (internal phase); Mix evenly and use it as the oil phase (external phase); add the water phase to the oil phase or add the oil phase to the water phase, stir evenly with a glass rod, and simply disperse the water phase in the oil phase to obtain the raw material of degradable magnetic beads microspheres ;

[0037] S2: alginate magnetic beads with a diameter of about 1 μm are prepared by ...

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Abstract

The invention relates to the fields of nano magnetic materials and biology, and discloses a preparation method of degradable immunomagnetic beads. Comprising the following steps: preparing a degradable magnetic bead microsphere raw material, preparing alginate magnetic bead microspheres by adopting a rapid membrane emulsification technology and the degradable magnetic bead microsphere raw material prepared in the step S1, and coupling the alginate magnetic bead microspheres with an antibody; the invention also discloses a degradable immunomagnetic bead. The invention further discloses application of the degradable immunomagnetic beads in products for separating human islet cell clusters. The immunomagnetic bead prepared by the invention can be specifically targeted to the pancreas islet in human pancreas, can realize separation of pancreas islet cell mass and exocrine tissue, can greatly shorten the pancreas islet separation time, improve the separation efficiency, improve the activity and function of the pancreas islet cell mass, improve the transplantation effect, and indirectly reduce the cost of pancreas islet transplantation; the prepared immunomagnetic beads are low in toxicity, good in magnetic response and good in surface biocompatibility, and can be degraded under the condition that the pH value of a solution is high.

Description

technical field [0001] The invention relates to the fields of nano-magnetic materials and biology, in particular to a degradable immunomagnetic bead and a preparation method thereof. Background technique [0002] Islet transplantation refers to the isolation and purification of the islet cell mass in the donor pancreas, which is usually transplanted into the liver of diabetic patients through the portal vein. progress. At present, the following problems and confusions exist in the field of islet transplantation: (1) There is a shortage of donors, and the number of islets required for transplantation is large (>11,000 IEQ / kg body weight); (2) It is difficult for the graft to survive in the host for a long time, and it is difficult for patients to obtain long-term insulin. Disengagement state; (3) Several problems of immunosuppression, etc. At present, the conventional clinical islet cell mass isolation process takes a long time, about 5-8 hours, and requires 4-5 people t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C12N5/071
CPCC07K16/18C12N5/0676C12N2509/00
Inventor 傅红兴余泳华刘成洋
Owner ZHEJIANG SHUREN UNIV
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