Artificial pancreas islet tissue and preparation and application thereof

A pancreatic islet, artificial technology, applied in tissue culture, biochemical equipment and methods, pancreatic cells, etc., can solve the problem of inevitable drug dependence, drug toxicity and side effects, etc.

Inactive Publication Date: 2019-07-02
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no cure for diabetes. For insulin-dependent patients, the only way is insulin drug injection. However, this method not only involves regular and quantitative injections every day, but also unavoidable drug dependence and drug side effects. It can be seen that the development of a simple and disposable islet tissue prosthesis with biological

Method used

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  • Artificial pancreas islet tissue and preparation and application thereof
  • Artificial pancreas islet tissue and preparation and application thereof
  • Artificial pancreas islet tissue and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1: the configuration of mixed culture medium

[0063] The composition of the mixed culture medium is: RPMI1640 + 10% FBS + 1% penicillin + 1% streptomycin, the culture medium is placed at 37°C, 5% CO 2 Equilibrate in the incubator for 2-4 hours.

Embodiment 2

[0064] Example 2: Obtaining pancreatic islet cells

[0065] The mice were sacrificed by neck dislocation, the entrance of the common bile duct to the duodenum was ligated, and the common bile duct was perfused with collagenase P 2.4 mg / mouse. After the pancreas was filled, the whole pancreas was carefully taken out; Shake and digest at 37°C for 12 minutes; add pre-cooled 10% FBS RPMI1640 medium after digestion to terminate digestion, shake vigorously for 15 seconds, then centrifuge at 4°C and 800 rpm for 4 minutes to remove supernatant; resuspend sediment in 10% FBS RPMI1640 medium and filter through a 50-mesh metal sieve , the filtrate was centrifuged at 800rpm at 4°C for 4min to remove the supernatant; resuspend the pellet in 10ml histopaque 1077 and slowly add 10ml serum-free RPMI1640 medium to make a clear layered interface between the two solutions; centrifuge at 2400rpm at 20°C for 20 minutes, and absorb the tissue between the two layers For the cell mass, wash 1-2 times...

Embodiment 3

[0066] Example 3: Preparation of 3D printed islet tissue

[0067] 1. Design and preparation of biological scaffolds

[0068] (1) Combining with computer aided design (CAD), three-dimensional printing technology is used to construct the bracket, in which the shape, composition and internal structure are well designable;

[0069] (2) The shape of the three-dimensional islet tissue is designed to be similar to that of the pancreas, and the internal structure is guaranteed to have a certain pore structure;

[0070] (3) The scaffold material is printed with gelatin compatibility.

[0071] (4) Internal structural parameters of the constructed three-dimensional islet tissue: pore size (R): = 0.2mm; line stacking angle: 90°.

[0072] (5) Store the prepared scaffold at -80°C for future use.

[0073] 2. Preparation of 3D artificial islet tissue

[0074] Each mouse pancreas contains 600-4000 islet tissues, and each islet tissue contains about 1000 islet cells. During printing, a thre...

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PUM

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Abstract

The present invention relates to an artificial pancreas islet tissue and particularly comprises a biocompatible stent, islet cells and a mixed culture solution. Through a 3D bioprinting technique, thebiocompatible stent and the pancreas islet tissue are prepared into the biologically active artificial pancreas islet tissue. The mixed culture solution is used for culture and preservation of the pancreas islet cells and tissue. The biocompatible stent is prepared using the 3D printing technique. The artificial pancreas islet tissue can be used as a pathophysiological mechanism research and a therapeutic tool for diabetes.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to artificial pancreatic islet tissue. Background technique [0002] Pancreatic islets are the endocrine part of the pancreas. They present ellipsoidal cell clusters of different sizes in the pancreas. The insulin and glucagon secreted by them are directly involved in the regulation of glucose metabolism in the body. Islet cells are mainly divided into α cells, β cells, γ cells and PP cells. Among them, β cells account for 60%-70% of pancreatic islet tissue and secrete insulin. Insulin can act on target organs such as liver, muscle, and fat, accelerate the storage and metabolism of glucose, and inhibit the production of glucose. It is the only hypoglycemic hormone in the human body. Insufficient secretion of insulin leads to the development of insulin-dependent diabetes mellitus (type I diabetes). [0003] At present, there is no cure for diabetes. For insulin-dependent patients, the...

Claims

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Application Information

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IPC IPC(8): C12N5/071A61P3/10A61P3/04
CPCA61K35/39C12N5/0677C12N2513/00
Inventor 张键薛丽赵华山黄晨杨雅莉汪宝蓓
Owner SHENZHEN INST OF ADVANCED TECH
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