Application of ISL1 in preparation of medicine for inhibiting early ferroptosis of transplanted pancreas islet and cell

Abstract

The invention relates to the field of pancreas islet transplantation, in particular to application of ISL1 in preparation of a medicine for inhibiting early ferroptosis of transplanted pancreas islet and a cell. Islet cells for overexpressing ISL1 are constructed by using the adenovirus to obtain islet cells for overexpressing ISL1. The expression change conditions of ferroptosis-related proteins (GPX4 and ACSL3) and apoptosis-related proteins are detected by using the overexpression and the knock-down of the ISL1 in the rat beta cell INS-1 through the lentivirus so as to determine that the ISL1 has the protection effect on the STZ induced INS-1 ferroptosis. The adenovirus of EcoRI MCS-ISL1 is utilized to construct rat and human islet cells for overexpressing ISL1, and it is clear that ISL1 inhibits transplanted islet ferroptosis by promoting expression of ACSL3 in the islet cells. A solid foundation is laid for improving survival of transplanted pancreas islet by genetically modified human pancreas islet cells and widely applying pancreas islet transplantation to clinic. The constructed cell can be used for experiments and pancreas islet transplantation possibilities.

Description

technical field [0001] The invention relates to the field of islet transplantation, in particular to the use and cells of ISL1 in the preparation of drugs for inhibiting early ferroptosis of transplanted islets. Background technique [0002] The survival of transplanted islets is one of the main factors affecting the early survival of transplanted islets [0003] Adult islet transplantation has always been a hot spot in the field of diabetes research. In 2014, according to the International Islet Transplantation Registry, more than 1,500 patients in clinical centers in about 40 countries have received islet transplantation. The curative effect after transplantation is comparable to that of pancreatic organ transplantation. The efficacy is comparable, and 50-70% of patients achieve insulin independence within 5 years. Although islet transplantation has achieved exciting results, there are still many deficiencies in the implementation of islet transplantation, which limit the...

AI Technical Summary

Problems solved by technology

However, two major issues that IBMIR will face in the future are: first, exploring the potential similarities and differences of IBMIR induced by using human-derived islet cells and porcine-derived islet cells in clinical islet transplantation; second, transplantation involving islet transplantation IBMIR only occurs in portal vein transplantation, and the relationship between IBMIR and extrahepatic transplantation site needs further study

However, the research on ISL1 in the ferroptosis of islet transplantation has not been reported at home and abroad, and further research is needed

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