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Zwitterionic Polypeptide/Gallic Acid/Iron Coordination Nanoparticles with Ultra-Small Particle Size and Its Preparation Method and Application

A zwitterion and gallic acid technology, applied in the field of biomedicine, can solve the problems of poor ferroptosis treatment effect, low photothermal conversion efficiency of ultra-small particle size and unsaturated iron coordination nanoparticles, low Fenton reaction activity, etc. , to enhance stability, accelerate Fenton reaction, and promote ferroptosis

Active Publication Date: 2022-05-17
SHANGHAI JIAOTONG UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the iron-gallic acid coordination polymer nano-network in the prior art cannot realize the preparation of ultra-small particle size and unsaturated iron coordination nanoparticles, low photothermal conversion efficiency, low Fenton reaction activity, poor ferroptosis treatment effect, As well as problems such as long-term biological toxicity, the present invention provides a zwitterionic polypeptide / gallic acid / iron coordination nanoparticle with an ultra-small particle size and its preparation method and application

Method used

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  • Zwitterionic Polypeptide/Gallic Acid/Iron Coordination Nanoparticles with Ultra-Small Particle Size and Its Preparation Method and Application
  • Zwitterionic Polypeptide/Gallic Acid/Iron Coordination Nanoparticles with Ultra-Small Particle Size and Its Preparation Method and Application
  • Zwitterionic Polypeptide/Gallic Acid/Iron Coordination Nanoparticles with Ultra-Small Particle Size and Its Preparation Method and Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Preparation of zwitterionic polypeptide / gallic acid / iron coordination nanoparticles with ultra-small particle size:

[0052] Step 1, take 2mL of zwitterionic polycysteine-g-(2-methacryloyloxyethylphosphorylcholine) aqueous solution (2mg / mL) in a 25mL glass bottle, slowly add 100μL of ferric chloride solution (40mg / mL), the solution was diluted to 5mL with deionized water, and stirred at room temperature for 12h.

[0053] Step 2: Add 50 μL of gallic acid solution (20 mg / mL) to the above solution, place on a vortex shaker and stir thoroughly for 5 min, then continue to react for 1 h with stirring at room temperature. After the reaction, dialyze with 10mmol / L PBS (pH=7.4) solution for 12h (2×1L, dialysis bag MW=1000), and dialyze to obtain zwitterionic polypeptide / gallic acid / iron coordination nanoparticles with ultra-small particle size Particles (synthetic roadmap such as figure 1 shown).

[0054] The dynamic light scattering spectrum of the zwitterionic polypeptide / g...

Embodiment 2

[0056] The light-to-heat conversion effect of the zwitterionic polypeptide / gallic acid / iron coordination nanoparticles with ultra-small particle size prepared in Example 1 was tested.

[0057] 200 μL of the zwitterionic polypeptide / gallic acid / iron complex nanoparticle solution (1.2mg / mL) with ultra-small particle size, at 1W / cm 2 The 808nm near-infrared light was irradiated, and the temperature of the solution was recorded every 30s through the thermocouple probe, and the light was cut off after 10min, and the temperature of the solution was recorded every 30s. Repeat this process 3 times. The heating and cooling curves of ultra-small particle size zwitterionic polypeptide / gallic acid / iron coordination nanoparticles (1.2mg / mL) under three cycles of light are shown in image 3 As shown, the temperature of the solution increases by about 27 °C, and it has good photothermal stability.

Embodiment 3

[0059] In vitro effect experiment of zwitterionic polypeptide / gallic acid / iron coordination nanoparticles with ultra-small particle size prepared in Example 1 on breast cancer cells.

[0060] The ultra-small particle size zwitterionic polypeptide / gallic acid / iron coordination nanoparticles (PCGA@FeNP) prepared in Example 1 were prepared in cell culture medium to concentrations of 2.34, 4.69, 9.38, 18.75, 37.5, 75, 150, 300 μg / mL, and then cultured with 4-T1 cells (breast cancer) for 72 hours, or after 12 hours of culture, and then illuminated with near-infrared laser for 10 minutes (808nm, 1W / cm 2 ), continue to culture for 48h. The cell viability test was carried out by the MTT method, and the results were as follows: Figure 4 As shown, the zwitterionic polypeptide / gallic acid / iron coordination nanoparticles with ultra-small particle size show a good ability of ferroptosis therapy to kill cancer cells, especially under near-infrared laser irradiation, which can further prom...

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Abstract

The invention relates to zwitterionic polypeptide / gallic acid / iron coordination nanoparticles with an ultra-small particle size and a preparation method and application thereof. Dissolving zwitterionic polycysteine-g-(2-methacryloyloxyethyl phosphorylcholine) in deionized water, adding an aqueous solution of ferric chloride, stirring at room temperature to allow a coordination reaction to occur; Adding gallic acid solution to the above solution, fully stirring, and performing dialysis after the reaction is completed, to obtain zwitterionic polypeptide / gallic acid / iron coordination nanoparticles with ultra-small particle size. Compared with the prior art, the preparation and reaction conditions of the zwitterionic polypeptide / gallic acid / iron coordination nanoparticles with ultra-small particle size in the present invention are mild, the design is reasonable, and the operation is simple and convenient. The zwitterionic polypeptide / gallic acid / iron coordination nanoparticles with ultra-small particle size obtained in the present invention realize the photothermal-ferroptosis combined treatment of breast cancer and its non-toxicity after one administration and one low dose of 808nm near-infrared light. Scar ablation has an important application prospect in the non-invasive treatment of breast cancer.

Description

technical field [0001] The invention belongs to the field of biomedical technology, and in particular relates to a zwitterionic polypeptide / gallic acid / iron coordination nanoparticle with an ultra-small particle size and a preparation method and application thereof. Background technique [0002] Ferroptosis is a new type of programmed cell death, which mainly induces cell death by catalyzing the highly expressed unsaturated fatty acids on the cell membrane through the Fenton reaction or ester oxygenase to induce lipid peroxidation. Ferroptosis therapy based on novel nanomaterials has attracted extensive attention in cancer therapy due to its unique antitumor therapeutic modality. At present, researchers have developed ferromagnetic nanomaterials, iron organic frameworks, iron nanocrystals, and iron coordination nanoparticles. Among them, iron-coordinated nanoparticles are expected to deliver iron ions to the tumor site, accelerate the Fenton reaction, and thus improve the e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K31/295A61K47/64A61K49/00A61K49/14A61K49/22A61P15/14A61P35/00B82Y5/00
CPCA61K41/0052A61K31/295A61K47/645A61P35/00A61P15/14A61K49/0002A61K49/0013A61K49/225A61K49/146B82Y5/00A61K2300/00
Inventor 杜畅董常明张蓉
Owner SHANGHAI JIAOTONG UNIV
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