Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

ga/fe 2+ Nanoparticles, their composite nanoparticles, preparation and application

A nanoparticle, 6H2O technology, applied in the field of medicine, can solve the problems of aseptic inflammation of acidic degradation products, aggravated neurotoxicity by initial burst release, and inability to significantly increase the drug loading capacity, so as to promote Fenton reaction, avoid brain tissue edema, The effect of improving bioavailability

Active Publication Date: 2022-04-22
SHANDONG UNIV QILU HOSPITAL
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Wafer has received more and more attention, but the inventors found that the slow-release chemotherapy system has exposed some defects in clinical application, such as: ①The drug loading is single and the drug loading cannot be significantly increased. If the drug loading is increased, it will lead to initial breakthrough ②Some patients may have obvious local cerebral edema; ③Acidic degradation products can easily cause aseptic inflammation; ④Degraded debris may block the circulation of cerebrospinal fluid

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • ga/fe <sup>2+</sup> Nanoparticles, their composite nanoparticles, preparation and application
  • ga/fe <sup>2+</sup> Nanoparticles, their composite nanoparticles, preparation and application
  • ga/fe <sup>2+</sup> Nanoparticles, their composite nanoparticles, preparation and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 GA / Fe 2+ Preparation of nanoparticles

[0050] FeCl 2 4H 2 O (23 mg) and PVP (80 mg) were added to 4 ml of degassed deionized water, and after stirring vigorously at room temperature for 5 minutes, GA (1 ml, 10 mg ml) dissolved in deionized water -1 ) was added dropwise into the above solution and stirred in a nitrogen atmosphere. After 24 hours, a purple GA / Fe 2+ nanocomposites. Concentrate and purify by ultrafiltration using an ultrafilter with a molecular weight cut off (MWCO) of 10 kDa. The resulting nanoparticles were stored at 4°C. GA / Fe 2+ The preparation process and function of nanoparticles are shown in figure 1 shown.

Embodiment 2

[0051] Example 2 GA / Fe 2+ Preparation of @ZIF-8 nanoparticles

[0052] This embodiment uses ZIF-8 package GOX and GA / Fe 2+ Granules, including: GA / Fe to be formulated with deionized water 2+ Nanoparticles (prepared by Example 1) solution (15mg ml -1 ) was added dropwise with stirring 160mM Zn(NO 3 ) 2 ·6H 2O aqueous solution, after stirring for 5 minutes, the mixed solution was added dropwise under vigorous stirring in the 2-methylimidazole aqueous solution of 10mM, after the reaction solution was centrifuged for 10 minutes, the precipitate obtained by washing with water was 3 times.

[0053] Among them, GA / Fe 2+ SEM and TEM images of @ZIF-8 nanoparticles figure 2 As shown, the particle size distribution and average particle size are as follows image 3 , Figure 4 As shown, the zeta potential is as Figure 5 shown.

Embodiment 3

[0054] Example 3 Drug-loaded GA / Fe 2+ @ZIF-8 nanoparticles

[0055] 1. GOX+GA / Fe 2+ Preparation of @ZIF-8 nanoparticles

[0056] GA / Fe prepared with deionized water 2+ Nanoparticles (prepared by Example 1) solution (15mg ml -1 ) was added dropwise with stirring 160mM Zn(NO 3 ) 2 ·6H 2 In O aqueous solution, the Zn(NO 3 ) 2 ·6H 2 GOX was dissolved in O aqueous solution, and the concentration of GOX was 1 mg / mL. After stirring for 5 minutes, the mixed solution was added dropwise to 10 mM 2-methylimidazole aqueous solution under vigorous stirring, and the reaction solution was centrifuged for 10 minutes, and then washed with water. The resulting precipitate was 3 times.

[0057] Among them, GOX+GA / Fe 2+ SEM and TEM images of @ZIF-8 nanoparticles figure 2 As shown, the particle size distribution and average particle size are as follows image 3 , Figure 4 As shown, the zeta potential is as Figure 5 shown.

[0058] 2. DOX+GOX+GA / Fe 2+ Preparation of @ZIF-8 nan...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a GA / Fe 2+ Nanoparticles, their composite nanoparticles, preparation and applications. The GA / Fe 2+ Nanoparticles include or are compounded from the following raw materials: Fe 2+ Salt, PVP and GA, the present invention adopts ZIF-8 encapsulation GA / Fe 2+ Nanoparticles loaded with drugs such as DOX and / or GOX to obtain composite nanoparticles can significantly increase the content of iron ions in tumor cells, and at the same time combine the loaded drugs to have a synergistic effect on tumor killing, promote Fenton reaction, and induce tumor cell ferroptosis , thereby effectively inhibiting the growth of tumor cells.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a GA / Fe 2+ Nanoparticles, their composite nanoparticles, preparation and applications. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Glioma is a cancer of the central nervous system with a high degree of malignancy and high local recurrence. Local chemotherapy has become one of the most promising methods for the treatment of glioma due to its definite therapeutic effect and small systemic side effects, and has been recognized by the latest edition of the United States. It is recommended by the NCCN guidelines, but it has not yet been clinically applied in China. The only local sust...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/52A61K47/22A61K47/12A61K47/61A61K47/69A61K33/26A61K31/704A61K38/44A61P35/00
CPCA61K9/5123A61K47/61A61K47/6929A61K33/26A61K31/704A61K38/44A61P35/00C12Y111/01A61K2300/00
Inventor 倪石磊张玉霖仲瀚林戚其超
Owner SHANDONG UNIV QILU HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com