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Drug-loaded microspheres for targeted inhibition of TNBC cell activity, and preparation method and application thereof

A cell activity, drug-loaded microsphere technology, applied in the field of molecular biology, can solve problems such as low inhibition efficiency, and achieve the effect of easy operation, stable structure, and promotion of phagocytosis

Pending Publication Date: 2022-05-17
川北医学院附属医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The first purpose of the present invention is to provide a drug-loaded microsphere that targets and inhibits the activity of TNBC cells, which can mainly solve the technical problem of low inhibition efficiency of inhibiting the activity of TNBC cells

Method used

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  • Drug-loaded microspheres for targeted inhibition of TNBC cell activity, and preparation method and application thereof
  • Drug-loaded microspheres for targeted inhibition of TNBC cell activity, and preparation method and application thereof
  • Drug-loaded microspheres for targeted inhibition of TNBC cell activity, and preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0035] In the second aspect, the present application also provides a method for preparing drug-loaded microspheres targeted to inhibit the activity of TNBC cells, including the following steps, using CPADB, EDC and sulfo-NHS to react to obtain an intermediate product, using NH 2 -1000DA-COOH reacts with the intermediate product and adds hydrogen chloride to obtain COOH-PEG-CPADB, dissolve COOH-PEG-CPADB in anhydrous dioxane, add BMA and V501 and seal it with a diaphragm, then pass N 2 , react at 65-75°C for 16-20h to obtain COOH-PEG-CPADB-[co-BMA], add PDSMA and V70 to COOH-PEG-CPADB-[co-BMA] and seal it with a diaphragm, then pass N 2 , reacted at 65-75°C for 16-20h to obtain COOH-PEG-CPADB-[co-BMA-co-PDSMA], that is, to obtain PC-coBP microspheres; dissolve the PC-coBP microspheres and take them out after standing The clear liquid is mixed with the loading agent to obtain a mixture, and water is added to the mixture, followed by vortex and ultrasonic treatment to obtain a cr...

Embodiment 1

[0043] This example provides a drug-loaded microsphere for targeted inhibition of TNBC cell activity, including a CD24 aptamer and a carrier, the CD24 aptamer is located on the surface of the carrier, the carrier is a composite microsphere, and the composite microsphere includes a microsphere body and a loading agent. The microsphere body is PC-coBP microspheres, and the loading agent includes NF2 knockout plasmid, YAP overexpression plasmid, ferroptosis agonist Erastin and 1-2M2ge, NF2 knockout plasmid, YAP overexpression plasmid and PC-coBP microspheres The weight ratio is 1.5:1.5:4.

[0044] This embodiment also provides a preparation method of drug-loaded microspheres targeting the inhibition of TNBC cell activity, including the following steps, using CPADB, EDC and sulfo-NHS to react to obtain an intermediate product, using NH 2 - PEG-COOH reacts with the intermediate product and adds hydrogen chloride to obtain COOH-PEG-CPADB, dissolve COOH-PEG-CPADB in anhydrous dioxane...

Embodiment 2

[0046]This example provides a drug-loaded microsphere for targeted inhibition of TNBC cell activity, including a CD24 aptamer and a carrier, the CD24 aptamer is located on the surface of the carrier, the carrier is a composite microsphere, and the composite microsphere includes a microsphere body and a loading agent. The microsphere body is PC-coBP microspheres, and the loading agent includes NF2 knockout plasmid, YAP overexpression plasmid, ferroptosis agonist Erastin and 1-2M2ge, NF2 knockout plasmid, YAP overexpression plasmid and PC-coBP microspheres. The weight ratio is 2:2:5.

[0047] This embodiment also provides a preparation method of drug-loaded microspheres targeting the inhibition of TNBC cell activity, including the following steps, using CPADB, EDC and sulfo-NHS to react to obtain an intermediate product, using NH 2 - PEG-COOH reacts with the intermediate product and adds hydrogen chloride to obtain COOH-PEG-CPADB, dissolve COOH-PEG-CPADB in anhydrous dioxane, ad...

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Abstract

The invention provides a drug-loaded microsphere for targeted inhibition of TNBC cell activity, and a preparation method and application thereof, and relates to the technical field of molecular biology. The microsphere comprises a CD24 aptamer and a carrier, the CD24 aptamer is located on the surface of the carrier, the carrier is a composite microsphere, the composite microsphere comprises a microsphere body and a loading agent, the microsphere body is a PC-coBP microsphere, and the technical problem that the inhibition efficiency of inhibiting TNBC cell activity is low can be mainly solved; the invention also provides a preparation method of the drug-loaded microspheres for targeted inhibition of TNBC cell activity, the preparation method is simple to operate, and batch production of the drug-loaded microspheres for targeted inhibition of TNBC cell activity is facilitated.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a drug-loaded microsphere for targeting and inhibiting the activity of TNBC cells, a preparation method and an application thereof. Background technique [0002] Breast cancer is the cancer with the highest morbidity and mortality in women. In recent years, the incidence and mortality of breast cancer in women are about 24.2% and 15%, respectively. With the use of various targeted anticancer drugs, although the 5-year relative survival rate of breast cancer has been greatly improved, the prognosis is still very unsatisfactory, especially for triple-negative breast cancer (TNBC). TNBC accounts for about 15-20% of breast cancers, and its aggressiveness, recurrence rate and mortality are higher than other types of breast cancer. Due to the lack of specific receptors in TNBC, most TNBC patients cannot benefit from targeted therapy. Standard chemotherapy is the only approv...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K47/54A61K45/00A61P35/00
CPCA61K47/549A61K47/6927A61K45/00A61P35/00
Inventor 侯令密邓世山王东生程攀科陈茂山梁骑蒲卢兰陈宇周瑜清苏小涵张振杨
Owner 川北医学院附属医院
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