Brain glioma targeting system based on magnetic nanoparticle composite engineered exosome and application of brain glioma targeting system

A magnetic nanoparticle, glioma technology, applied in the field of glioma targeting system, can solve the problem of not greatly prolonging the survival time of patients, and achieve the effect of promoting ferroptosis and targeted therapy for glioma

Active Publication Date: 2022-07-08
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, the standard treatment for glioblastoma consists of surgery followed by simultaneous oral temozolomide chemotherapy and radiation therapy. Other treatment modalities such as gene therapy, immunotherapy, and even tumor electric field therapy (TTFields) have not significantly prolonged the patient's survival time

Method used

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  • Brain glioma targeting system based on magnetic nanoparticle composite engineered exosome and application of brain glioma targeting system
  • Brain glioma targeting system based on magnetic nanoparticle composite engineered exosome and application of brain glioma targeting system
  • Brain glioma targeting system based on magnetic nanoparticle composite engineered exosome and application of brain glioma targeting system

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preparation example Construction

[0058] In another specific embodiment of the present invention, there is provided a preparation method of the above-mentioned glioma targeting system, the preparation method comprising:

[0059] S1. Constructing magnetic nanoparticles;

[0060] S2. Construction of engineered exosomes;

[0061] S3. The magnetic nanoparticles are combined with the engineered exosomes to form a complex.

[0062] Wherein, in the step S1, the method includes: constructing magnetic nanoparticles with iron oxide particles as base cores and mesoporous silica as shells;

[0063] Specific methods include:

[0064] S1.1 Synthesis of iron oxide nanoparticles: Dissolve ferric chloride and oleic acid in a mixed solution of absolute ethanol, deionized water and n-hexane, and react at a constant temperature of 60-80 °C for 1-5 h (preferably at a constant temperature of 70 °C for 4 h) ); after extracting the product and drying, a solid iron oleate mixture was obtained, and iron oleate and oleic acid were mi...

Embodiment

[0083]A glioma targeting system based on magnetic nanoparticle composite engineered exosomes, the construction method is as follows:

[0084] Construction of Magnetic Nanoparticles: Construct magnetic nanoparticles with iron oxide particles as the base core and silica as the shell. Fe 3 O 4 Synthesis of nanoparticles: Synthesis of iron oxide nanoparticles: Dissolve 10.8g of ferric chloride and 36.5g of sodium oleate in a mixed solution of 80mL of absolute ethanol, 60mL of deionized water and 140mL of n-hexane, and react at a constant temperature of 70°C for 4h; The product was extracted and dried to obtain a solid iron oleate mixture. The obtained iron oleate was mixed with 5.7g of oleic acid, dissolved in 200g of 1-octadecene in a nitrogen environment, and the above reaction system was heated to a constant temperature of 320°C for 30min. , the solution changed from initial transparent to brown-black; cooled to room temperature; obtained by centrifugal precipitation;

[008...

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Abstract

The invention belongs to the technical field of bioengineering technologies, and particularly relates to a brain glioma targeting system based on a magnetic nanoparticle composite engineering exosome and application of the brain glioma targeting system. The brain glioma targeting system comprises magnetic nanoparticles and an engineered exosome, wherein the magnetic nanoparticles and the engineered exosome are connected through an antigen-antibody reaction. According to the designed composite targeting system for targeting glioma ferroptosis, the magnetic targeting characteristic and the drug binding capacity of the magnetic nanoparticles (MNPs) are combined with the blood brain barrier (BBB) penetrating capacity and the small interfering RNA encapsulation capacity of the engineered exosome. According to the invention, through the combined targeting DHODH and GPX4 pathways and the synergistic effect with Fe < 2 + > ions released by Fe3O4, ferroptosis of glioma is promoted together, and the purpose of targeted treatment of brain glioma is achieved, so that the application has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a glioma targeting system based on magnetic nanoparticle composite engineered exosomes and its application. Background technique [0002] The information disclosed in this Background of the Invention is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] Glioblastoma (GBM) is one of the most aggressive brain tumors with a poor prognosis. Currently, the standard treatment for glioblastoma consists of surgery followed by concurrent oral temozolomide chemotherapy and radiation therapy, and other treatment modalities such as gene therapy, immunotherapy, and even tumor field therapy (TTFields) have not significantly prolonged patient survival. The m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K47/68A61K47/62A61K41/00A61K31/47A61K31/713A61P35/00A61P25/00A61D7/00A61N2/00A61N2/08
CPCA61K47/6949A61K47/6849A61K47/6901A61K47/62A61K41/00A61K31/47A61K31/713A61P35/00A61P25/00A61N2/06A61N2/002A61D7/00A61K2300/00Y02A50/30
Inventor 薛皓李柏延桑元华陈鑫赵荣荣仇吉川刘宏薛付忠李刚
Owner SHANDONG UNIV QILU HOSPITAL
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