361results about How to "The value of good practical application" patented technology

The invention provides an epilepsy medication recommendation method and system, and belongs to the technical field of biomedicine and data processing. According to the invention, deep sequencing is carried out on an antiepileptic drug metabolic enzyme gene, a transporter gene and a drug action target gene, related coding gene polymorphic sites are screened, a Bayesian accumulation regression treemodel and random forest regression are utilized to obtain a personalized medication scheme depending on individual genes, and a subset with high treatment effect is identified, so that clinical medication is guided in a targeted manner, the epilepsy control rate is improved, adverse reactions are reduced, the time for doctors and patients to explore the optimal treatment scheme is shortened, the diagnosis and treatment efficiency is improved, and the method has good practical application value.

The invention provides a personalized adverse drug reaction prediction method, system and device and a medium, and belongs to the technical field of biomedicine. The invention provides a multi-task learning model (KEMULA) based on multi-kernel function learning so as to replace traditional learning methods of universal application and complete individuation. More specifically, the model calculatesand ranks the risks of ADR development of patients by learning a constrained personalized ADR ranking function by assuming a sharing function of the model. This function is referred to as a personalized ADR ranking function, which is a linear combination of several scoring functions that calculate the risk of developing related ADR of patients. In addition, the model is also combined with Laplacian regularization to ensure that variable information trained by personADRank functions of similar patients is close, so that the causal relationship (true positive) of the model to the association between a given patient and the corresponding ADR can be improved. Therefore, the method has good practical application value.

The invention provides a photoelectrochemical biosensor as well as a preparation method and application thereof, and belongs to the technical field of detection and analysis. The photoelectrochemicalbiosensor comprises: an electrode, which comprises an electrode substrate and a covalent organic polymer film coating the electrode substrate, wherein the covalent organic polymer film is modified with palladium nanoparticles and an aptamer; and the covalent organic polymer film is prepared by carrying out a polymerization reaction on 2,6-dihydroxynaphthalene-1,5-diformaldehyde and tris(4-aminophenyl)amine. The cathode photoelectrochemical cell sensor is successfully constructed by in-situ synthesis of the covalent organic polymer film, can be used for detecting various cells such as cancer cells by changing the type of the aptamer, and has high sensitivity and strong specificity, so that the cathode photoelectrochemical cell sensor has a good practical application value.

The invention provides a molecular marker for diagnosing or treating glioma and applications thereof. Experimental studies show that the expression of microRNA-588 in glioma is significantly up-regulated in hypoxia state than in normoxia state. In vitro experiments show that the miR-588 can inhibit the invasive migration of glioma cells and VM formation ability. In order to clarify the molecular mechanism, further experiments find that Robo1 is a direct and functional related target of the miR-588 in glioma. The knocking out of the Robo1 inhibits the expression of matrix metalloproteinase 2 (MMP2) and matrix metalloproteinase 9 (MMP9), thereby inhibiting invasive migration of glioma and VM formation ability. Therefore, the miR-588 and the Robo1 can be used as a molecular marker for diagnosing or treating glioma.

The invention provides an ulcerative colitis biomarker based on intestinal flora and application of the ulcerative colitis biomarker, and belongs to the technical field of biological medicines. The ulcerative colitis biomarker based on intestinal flora provided by the invention can overcome the defects that the existing ulcerative colitis diagnosis cannot realize early warning, cannot predict themorbidity and development trend and the like; and the ulcerative colitis biomarker based on intestinal flora can help pathological typing of diseases and research on drug action targets, precise medication, pathogenesis and the like, so that a high practical application value is achieved.

The invention provides a method and a kit for simultaneously detecting various metabolites on a homocysteine metabolic pathway and application of the method and the kit, and belongs to the technical field of metabolite detection. The method comprises the following steps: detecting a pretreated sample to be detected on the basis of LC-MS/MS; wherein the pretreatment method comprises the step of adding vitamin C into a sample to be detected. By optimizing a pretreatment method, accurate detection of seven substances such as homocysteine, folic acid, 5-methyltetrahydrofolic acid, methionine, vitamin B6, vitamin B12 and cysteine is realized, and the method can be applied to detection items of important metabolites on a clinical homocysteine metabolic pathway. The real level of the real concentration of the metabolite in a subject can be provided, clinical use or laboratory use is facilitated, and the method has important guiding significance on prevention or treatment of hyperhomocysteinemia, so that the method has good practical application value.

The invention provides bacillus velezensis as well as a microbial inoculum and application thereof, and belongs to the technical field of microorganisms. The biocontrol bacterium Bacillus velezensis GY1 is screened from rhizosphere soil of healthy tobaccos, has a remarkable antagonistic effect on various phytopathogens in the agricultural production, is wide in antibacterial spectrum, and is capable of effectively controlling tobacco mildew and rot and tobacco brown spot expansion in the baking period through testing, and therefore, the tobacco quality is improved, the tobacco use value is improved, so that the bacillus velezensis has wide application prospect and good practical application value in the field of biological prevention and control of plant diseases.

The invention provides a temperature-resistant thick oil viscosity-reducing polymer as well as a preparation method and application thereof, and belongs to the technical field of thick oil viscosity-reducing agents. The temperature-resistant thick oil viscosity-reducing polymer is prepared from the following raw materials: an acrylamide monomer, a sodium acrylate monomer, a hydrophobic monomer, a temperature-resistant functional monomer, a micro-crosslinking monomer, an initiator, a temperature stabilizer and water. The temperature-resistant thick oil viscosity-reducing polymer prepared by the invention not only has good emulsifying performance and thick oil affinity performance, but also has good temperature resistance, is particularly suitable for high-temperature thick oil reservoir development, and has good practical production and application values.

The invention provides a method and a system for detecting COVID-2019 pneumonia, which belongs to the technical field of biomedicine and data processing. Expiratory NO concentration detection is carried out on a non-new coronal pneumonia patient, statistics of basic human body characteristic information is carried out, a mathematical model between the expiratory NO concentration and the basic characteristics of the human body is established, expiratory NO data of the suspected new coronal pneumonia patient are analyzed by using the mathematical model, whether a subject is sick or not is thus judged, the problem of discomfort of a human body caused by traditional nucleic acid detection sampling is effectively solved, and the method has the advantages of low cost, rapidness, no damage and the like, causes no pain to the subject, is good in compliance, is particularly suitable for being applied to large-scale preliminary screening work of suspected COVID-19 patients, and has good practical application value.

The invention provides a composite microbial agent and a preparation method and application thereof, and belongs to the technical field of microorganisms. The composite microbial agent is prepared from the following components in parts by weight: 1-2 parts of paecilomyces lilacinus, 0.5-1.5 parts of paenibacillus polymyxa, 3-10 parts of calcium lignosulphonate, 2-8 parts of sodium carboxymethyl cellulose, 7-17 parts of tobacco powder, 8-15 parts of auxiliary beauty powder, 3-7 parts of starch, 8-16 parts of a composite synergist, 5-15 parts of diatomite, 7-14 parts of attapulgite and 23.5-35.5parts of water. Paecilomyces lilacinus and paenibacillus polymyxa are combined for use, soil-borne diseases and insect pests such as root-knot nematode, Chinese cabbage soft rot, pepper root rot, tomato seedling blight and pepper phytophthora blight can be effectively prevented and treated, and the product is non-toxic, pollution-free, safe and efficient and is beneficial to maintaining ecological balance and is an effective substitute for part of pesticides abd meets the requirements of agricultural sustainable development and has good practical application value.

The invention provides a molecular biomarker for diagnosing and treating triple-negative breast cancer and application thereof, and belongs to the technical field of crude drugs and molecular biology.The invention finds for the first time that circEIF3H expression in a tumor specimen of a patient suffering from triple-negative breast cancer is obviously higher than that of breast cancer tissues of other subtypes, meanwhile, the circEIF3H expression quantity is related to prognosis of triple-negative breast cancer, and malignant growth and invasion capacity of breast cancer cells can be controlled by regulating the circEIF3H expression. Therefore, the circEIF3H can be used as a molecular biomarker for diagnosing and treating the triple-negative breast cancer, and can be used as a prognosisindex and a potential treatment target of the triple-negative breast cancer. The biomarker provides a more favorable means for diagnosis and prognosis evaluation analysis of the triple-negative breast cancer, has important significance for research and treatment of the triple-negative breast cancer, and thus, has favorable practical application value.

The invention provides a method for ynthesis of (S)-rivastigmine through reductive amination, belonging to the technical field of synthesis of chiral medicines in the pharmaceutical and chemical industry. The synthetic method of the invention comprises the following steps: with 3-hydroxyacetophenone as a raw material, carrying out a simple condensation reaction so as to realize high-yield preparation of 3-nitromethylethylformate acetophenone; then with 3-nitromethylethylformate acetophenone as a substrate, carrying out an asymmetric reductive amination reaction with benzhydrylamine under the catalysis of an Ir complex so as to obtain a chiral amine precursor with high enantioselectivity and high yield; and then carrying out deprotection and a methylation reaction so as to obtain (S)-rivastigmine with high enantioselectivity and high yield. The synthetic route of the method is short; the total yield of four steps can reach 80%; the enantioselective excess of (S)-rivastigmine can reach 98%; so the method has good industrial application prospects and practical application value.

The invention provides an inactivated lactic acid bacteria inoculant as well as a preparation method and application thereof, and belongs to the technical field of microbial preparations. Two strainsof lactic acid bacteria, namely lactobacillus paracasei HYZQF3DS-2 and lactobacillus plantarum HYZQF1675-63, are obtained through screening and further mutagenesis treatment, the two strains of lacticacid bacteria are very suitable for thermal inactivation treatment, and fermentation process research is further carried out on the two strains of lactic acid bacteria, thereby improving the strain fermentation performance; and experiences prove that the microbial inoculum obtained after the fermentation culture of the two strains has good effects of relieving constipation and diarrhea; a meanwhile, the bacteria inoculant has the effects of improving sleep disorder, anxiety and the like, thereby having good practical application value.

The invention provides an SN-38-PLGA sustained release microsphere for intratumoral injection and a preparation method and application thereof. SN-38 is used as a model drug, PLGA is used as a carriermaterial, the SN-38-PLGA sustained release microsphere capable of being available for intratumoral injection dose is successfully built by adopting an emulsification-solvent evaporation method, and the SN-38-PLGA sustained release microsphere has the advantages of smooth and rounded surface, moderate particle size and high encapsulation efficiency and drug-loading capacity and the like; and meanwhile, the SN-38-PLGA sustained release microsphere for the intratumoral injection can enable the SN-38 to maintain effective high concentration for a long time in a tumor area, inactivation of the SN-38 drug is effectively avoided, and thus good practical application value is achieved.

The invention provides a bionic nano preparation based on platelet membrane fragments as well as a preparation method and application of the bionic nano preparation, and belongs to the technical fieldof medicine. By means of drug combination of a cyclin dependent kinase 5(Cdk5) inhibitor and a chemotherapeutic drug, on one hand, tumor cells are killed with chemotherapy, the immune system is activated, content of IFN-gamma is recovered, immunosuppression caused by postoperative stress is relieved, on the other hand, PD-L1 is down-regulated by the Cdk5 inhibitor, IFN-gamma related immune drug resistance is relieved, and through combination of the Cdk5 inhibitor and the chemotherapeutic drug, dual characters of IFN-gamma are changed, the downstream immunosuppressive action of the IFN-gamma is removed, and the positive effect of IFN-gamma is exerted; and meanwhile, the problem of postoperative targeted drug delivery is solved according to the biological trend characteristic of platelets,besides, with improvement of the preparation method, the preparation method of bionic nanoparticles based on the platelet membrane fragments is simple, the drug-loading efficiency is high, and therefore, the bionic nanoparticles have good practical application value.

The invention provides hydrogel capable of loading a high-hydrophobicity drug as well as a preparation method and application of the hydrogel, which belongs to the technical field of preparation of high polymer materials. The preparation method comprises the following steps of firstly, preparing cyclodextrin-terminated polyurethane (DPU) with a side chain containing double bonds, and carrying out click chemical crosslinking on a multi-sulfhydryl compound and the double bonds to form the polyurethane hydrogel. The inner cavity of cyclodextrin is hydrophobic, the outer edge of the cyclodextrin is hydrophilic, and a hydrophobic binding site can be provided, so that the cyclodextrin molecular cavity in the polyurethane hydrogel can be subjected to non-covalent binding with a high-hydrophobicity drug, the loading of the high-hydrophobicity drug is realized, the gel is very stable in vitro, and under the action of an in-vivo enzyme, the cyclodextrin polysaccharide is gradually degraded, so that the combined medicine is released, the hydrogel capable of loading the high-hydrophobicity drug is successfully prepared, and the hydrogel has a good practical application value.

The invention provides a method for screening a bovine plateau hypoxia-adapted gene ALDOC and a functional molecular marker and application of the bovine plateau hypoxia-adapted gene ALDOC and functional molecular markers, and belongs to the technical field of animal molecular breeding. According to the invention, cattle varieties distributed at high altitude and low altitude are selected from the perspectives of cattle genome selection, genetic adaptation and the like, and genome selection signals between cattle at high altitude and cattle at low altitude and in the cattle varieties are compared by adopting an SNP (Single Nucleotide Polymorphism) chip and integrating three genome selection signal analysis methods of FLK, hapFLK and XPEHH; candidate genes adapting to the plateau hypoxic extreme environment are identified through a combined screening strategy and bioinformatics analysis, potential functional molecular markers are further mined through selection signal verification and re-sequencing data analysis, and a corresponding detection method is established. A scientific basis and a simple and feasible detection technology are provided for molecular breeding such as cultivation of plateau hypoxic characteristic cattle varieties; meanwhile, the method has important significance and value for protection, evaluation and utilization of genetic resources of local cattle varieties.

The invention provides application of urolithin A and derivatives thereof in tumor immunotherapy, and belongs to the technical field of biological medicine and molecular biology. According to the application, the research results show that the urolithin A and the derivatives thereof can promote the effector function of CD8 + T cells and other TCR pathway mediated activated effector T cells or artificially prepared effector T cells (including but not limited to CAR-T and TCR-T cells) so as to improve the tumor immunotherapy effect, such that the understanding of the urolithin A is effectively broadened. Regulation and control of the urolithin A on CD8 + T cell functions further reveals interaction of flora metabolites and an immune system, a theoretical basis is provided for deeply excavating more metabolites for regulating and controlling immunity, a promising immunotherapy strategy is provided for tumor patients, and therefore the urolithin A has good practical application value.