Method for screening bovine plateau hypoxia-adapted gene ALDOC and functional molecular markers and application of bovine plateau hypoxia-adapted genes ALDOC and functional molecular markers

Abstract

The invention provides a method for screening a bovine plateau hypoxia-adapted gene ALDOC and a functional molecular marker and application of the bovine plateau hypoxia-adapted gene ALDOC and functional molecular markers, and belongs to the technical field of animal molecular breeding. According to the invention, cattle varieties distributed at high altitude and low altitude are selected from the perspectives of cattle genome selection, genetic adaptation and the like, and genome selection signals between cattle at high altitude and cattle at low altitude and in the cattle varieties are compared by adopting an SNP (Single Nucleotide Polymorphism) chip and integrating three genome selection signal analysis methods of FLK, hapFLK and XPEHH; candidate genes adapting to the plateau hypoxic extreme environment are identified through a combined screening strategy and bioinformatics analysis, potential functional molecular markers are further mined through selection signal verification and re-sequencing data analysis, and a corresponding detection method is established. A scientific basis and a simple and feasible detection technology are provided for molecular breeding such as cultivation of plateau hypoxic characteristic cattle varieties; meanwhile, the method has important significance and value for protection, evaluation and utilization of genetic resources of local cattle varieties.

Description

Technical field[0001]The present invention belongs to the technical field of animal molecular breeding, and more particularly to a method of screening a low oxygen adaptive gene ALDOC and a functional molecular label in a cow plateau.Background technique[0002]The information disclosed in the background is only intended to increase the understanding of the overall background of the present invention, and is not necessarily considered to recognize or imply that the information is intended to be a prior art known to those of ordinary skill in the art.[0003]Cattle is a ruminant, and they live in different environments, with the ability to effectively transform low quality feed into high-energy fat, meat and milk (Elsik et al, 2009). Under the action of tumes, the crude feed is digested as fermentation products such as acetic acid, butyric acid and propionic acid (Russell and Van Soest, 1984). The high altitude of the Qinghai-Tibet Plateau will weaken the fermentation of lytic and improv...

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Problems solved by technology

[0005] High-altitude areas are characterized by hypoxia (air oxygen content is only 50%-60% of that in plain areas), low annual average temperature (from -1°C to -5°C), and short growing seasons (from June to September) and seasonal changes in forage supply (Shao et al., 2010), resulting in a lack of quality forage

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