The invention relates to a preparation method for
porphyra yezoensis
sesquiterpene algal activity-inhibiting compounds. Dry
porphyra yezoensis
powder is added with absolute
methanol and extracted, andafter
drying by
vacuum evaporation, extract is obtained; the extract is added with methyl
alcohol for
alcohol precipitation, so that supernate is obtained; the supernate is extracted by
ethyl acetate, so that alga-inhibiting components B, C and D are obtained,
silica gel column chromatography is adopted to carry out separation, so that alga-inhibiting components B2, B4, C1, C2, D3 and D4 are obtained,
silica gel column chromatography is adopted to carry out separation again, so that alga-inhibiting components B21, B23, B41, B42, C11 and C12 are obtained, and except that the component B21 continues to undergo separation by
silica gel column chromatography, the rest of the components adopt silica gel thin-layer
chromatography to carry out preparation; and by separation, five thin-layer purealga-inhibiting
sesquiterpene compounds are obtained. The invention adopts an
activity tracking method to purify and prepare the
sesquiterpene compounds with
red tide microalga-inhibiting activity from
porphyra yezoensis by methods such as methyl
alcohol extraction, liquid-liquid extraction, silica gel column
chromatography and thin-layer
chromatography preparation. The design of the method is reasonable, the
operability is high, and the microalge-inhibiting activity of the prepared compounds is good.