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Preparation method for porphyra yezoensis sesquiterpene algal activity-inhibiting compounds and application

A technology of Porphyra variegata and sesquiterpenoids, which is applied to the preparation of the above-mentioned compounds, algae-inhibiting active compounds, and the preparation of algae-inhibiting active compounds of Porphyra variegata sesquiterpenoids, and can solve the problems that have not yet been seen. Separation, purification and structural identification of algae-inhibiting active substances, etc., to achieve the effect of good antimicrobial activity, reasonable method design, and strong operability

Inactive Publication Date: 2018-08-17
JIANGSU MARINE RESOURCES DEV RES INST LIAN YUNGANG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, there is no separation, purification and structural identification of algae-inhibiting active substances from Porphyra zebra at home and abroad.
Therefore, whether there are sesquiterpenoids with anti-algae activity in P.

Method used

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  • Preparation method for porphyra yezoensis sesquiterpene algal activity-inhibiting compounds and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1. A preparation method of Porphyra yezoensis sesquiterpenoid algae inhibitory active compound:

[0018] (1) Porphyra variegata dry powder is added to anhydrous methanol and extracted at 15-20°C for 12 hours to obtain an extract. Repeat the extraction 3 times and combine the extracts. After filtering and evaporating under reduced pressure, Porphyra yezoensis extract is prepared. This extract was added with 90% methanol aqueous solution, fully shaken, and allowed to stand overnight at 4°C, centrifuged and filtered to remove the precipitate and retain the supernatant. Take an appropriate amount of this supernatant and evaporate to dryness under reduced pressure, and then dissolve it in methanol. The methanol solution is tested for its anti-algae activity. The results show that it significantly inhibits the growth of Karenia mikimotoi;

[0019] (2) Adjust the pH of the supernatant to 11, add ethyl acetate and extract 3 times. The upper layer was evaporated to dryness...

Embodiment 2

[0021] Example 2. A preparation method of Porphyra yezoensis sesquiterpenoid algae inhibitory active compound:

[0022] Porphyra yezoensis was pulverized into a 0.3 mm powder, added with anhydrous methanol, and extracted at 15-20°C for 12 hours, and then extracted repeatedly 3 times, and the extracts were combined. After filtering and evaporating under reduced pressure, Porphyra yezoensis extract is prepared. This extract was added with 90% methanol aqueous solution, fully shaken, and allowed to stand overnight at 4°C, centrifuged and filtered to remove the precipitate and retain the supernatant. Take an appropriate amount of this supernatant, evaporate to dryness under reduced pressure at 60°C, and then dissolve it in methanol for the detection of algal inhibitory activity (in the present invention, unless otherwise specified, the method for detection of algal inhibitory activity is the same). In a 96-well plate, 1 μL of the test component solution was added to 199 μL of algae ...

Embodiment 3

[0024] Example 3, a preparation method of Porphyra yezoensis sesquiterpenoid algae inhibitory active compound:

[0025] Weigh 1500 g of dried Porphyra yezoensis powder, add 3.0 L of anhydrous methanol, and extract at room temperature (15-20°C) for 12 hours, then pour out the extract. Add fresh anhydrous methanol again and extract under the same conditions as above. After repeating 2 times, combine the extracts (3 extractions in total). After filtration and evaporation under reduced pressure, 34.74 g of Porphyra yezoensis extract was prepared. Add 200 mL of 90% methanol aqueous solution to this extract, vortex thoroughly, let it stand overnight at 4°C, centrifuge, filter, remove the precipitate, and obtain 180 mL of supernatant.

[0026] Take 1 mL of the supernatant, evaporate to dryness under reduced pressure at 60°C, and dissolve it in 20 mL of methanol to prepare a 10 g / L mother liquor for the detection of algal inhibitory activity. In a 96-well plate, add 1 μL of the test com...

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Abstract

The invention relates to a preparation method for porphyra yezoensis sesquiterpene algal activity-inhibiting compounds. Dry porphyra yezoensis powder is added with absolute methanol and extracted, andafter drying by vacuum evaporation, extract is obtained; the extract is added with methyl alcohol for alcohol precipitation, so that supernate is obtained; the supernate is extracted by ethyl acetate, so that alga-inhibiting components B, C and D are obtained, silica gel column chromatography is adopted to carry out separation, so that alga-inhibiting components B2, B4, C1, C2, D3 and D4 are obtained, silica gel column chromatography is adopted to carry out separation again, so that alga-inhibiting components B21, B23, B41, B42, C11 and C12 are obtained, and except that the component B21 continues to undergo separation by silica gel column chromatography, the rest of the components adopt silica gel thin-layer chromatography to carry out preparation; and by separation, five thin-layer purealga-inhibiting sesquiterpene compounds are obtained. The invention adopts an activity tracking method to purify and prepare the sesquiterpene compounds with red tide microalga-inhibiting activity from porphyra yezoensis by methods such as methyl alcohol extraction, liquid-liquid extraction, silica gel column chromatography and thin-layer chromatography preparation. The design of the method is reasonable, the operability is high, and the microalge-inhibiting activity of the prepared compounds is good.

Description

Technical field [0001] The present invention and a preparation method of an active compound for inhibiting algae, especially a method for preparing a sesquiterpenoid active compound for inhibiting algae from Porphyra yezoensis. The present invention also relates to the use of the aforementioned compound, which belongs to the field of marine biochemical engineering. Background technique [0002] Sesquiterpenes are a class of terpenoids with a 15-carbon skeleton. They are widely distributed in nature and have strong anti-tumor, anti-bacterial, anti-viral and anti-malarial activities. However, the activity of sesquiterpenoids isolated from seaweeds is rarely reported, and only the genus Aspergillus ( Laurencia It is reported that the sesquiterpenoids in sp. have anti-tumor, antibacterial and antiviral activities. For example, the three rows of concave top algae ( Laurencia tristicha ), Okamura Atopia ( Laurencia okamurai Yamada), L. Claviformis , L . Majuscula , L. yonagunien...

Claims

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Application Information

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IPC IPC(8): C07C33/30C07C35/06C07C35/36C07C29/86C07C29/76C07D307/12A01N31/04A01N31/06A01N43/08A01P13/00
CPCA01N31/04A01N31/06A01N43/08C07C29/76C07C29/86C07C2601/08C07C2602/26C07D307/12C07C33/30C07C35/06C07C35/36
Inventor 孙颖颖周文静徐嘉韩伟孙菁雯王长萍张新
Owner JIANGSU MARINE RESOURCES DEV RES INST LIAN YUNGANG
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