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Application of urolithin A and derivatives thereof in tumor immunotherapy

A technology of urolithin and derivatives, applied in the fields of biomedicine and molecular biology, can solve the problems of function regulation and anti-tumor that have not been reported yet.

Active Publication Date: 2021-12-21
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But urolithin A on CD8 + Functional regulation and anti-tumor effects of T cells have not been reported yet

Method used

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  • Application of urolithin A and derivatives thereof in tumor immunotherapy
  • Application of urolithin A and derivatives thereof in tumor immunotherapy
  • Application of urolithin A and derivatives thereof in tumor immunotherapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0066] experimental method

[0067] 1. Construction of TCR pathway screening system based on Jurkat-NFATLuc cells

[0068] (1) Construct the NFATLuc expression plasmid pultra-BSD-NFATLuc, transfer pultra-BSD-NFATLuc into Jurkat T cells (acute T-cell leukemia cells) to construct the Jurkat-NAFTLuc cell line stably expressing NFATLuc, and use Blasticidin for screening;

[0069] (2) Pretreat Jurkat-NAFTLuc cells with metabolites for 2 hours, and add hCD3, hCD28 and rabbit anti-mouse IgG cross-linked antibodies to stimulate for 4 hours after 2 hours;

[0070] (3) Add 10XFLuc-Lysis Lysis Buffer and lyse at room temperature for 30 minutes;

[0071] (4) Add FLuc Assay Working Solution, and use a microplate luminometer (LB960) to detect the intensity of fluorescence to indicate the degree of TCR activation.

[0072] 2. Western Blot detection of phosphorylated proteins in the TCR pathway

[0073] (1) Collect urolithin A and control-induced CD8 + T cells;

[0074] (2) Centrifuge th...

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Abstract

The invention provides application of urolithin A and derivatives thereof in tumor immunotherapy, and belongs to the technical field of biological medicine and molecular biology. According to the application, the research results show that the urolithin A and the derivatives thereof can promote the effector function of CD8 + T cells and other TCR pathway mediated activated effector T cells or artificially prepared effector T cells (including but not limited to CAR-T and TCR-T cells) so as to improve the tumor immunotherapy effect, such that the understanding of the urolithin A is effectively broadened. Regulation and control of the urolithin A on CD8 + T cell functions further reveals interaction of flora metabolites and an immune system, a theoretical basis is provided for deeply excavating more metabolites for regulating and controlling immunity, a promising immunotherapy strategy is provided for tumor patients, and therefore the urolithin A has good practical application value.

Description

Technical field [0001] The invention belongs to the technical fields of biomedicine and molecular biology, and specifically relates to the application of urolithin A and its derivatives in tumor immunotherapy. Background technique [0002] The information disclosed in the Background of the Invention is merely intended to increase understanding of the general background of the invention and is not necessarily to be regarded as an admission or in any way implying that the information constitutes prior art that is already known to a person of ordinary skill in the art. [0003] Numerous studies have shown that the microbiome can influence cancer development. The composition of the gut microbiome is altered in a variety of diseases, including cancer. Preclinical studies have demonstrated that modulating the gut microbiome can improve host responsiveness to cancer treatments. In addition, microbial agents or related products for treating cancer have the potential to shrink tumo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/37A61K39/395A61K35/17A61P35/00A61P35/04A61P35/02
CPCA61K31/37A61K39/3955A61K35/17A61P35/00A61P35/04A61P35/02A61K2300/00Y02A50/30
Inventor 李春阳马春红马帅雅陈朝佳高立芬梁晓红
Owner SHANDONG UNIV
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