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Inactivated lactic acid bacteria inoculant as well as preparation method and application thereof

A technology of lactic acid bacteria and bacterial agents, applied in biochemical equipment and methods, bacteria used in food preparation, applications, etc., can solve problems affecting product effects, lactic acid bacteria cell breakdown, cell adhesin shedding, and increased production costs, etc., to achieve Improve equipment utilization, comprehensively enrich metabolites, and make it easier to operate

Active Publication Date: 2020-12-11
山东环亿生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There are many advantages and disadvantages in the use of inactivated lactic acid bacteria. The current inactivation methods are all heat inactivation, but this method will cause a large number of lactic acid bacteria cells to break up and cell adhesions to fall off, because the inactivated lactic acid bacteria cells can only guarantee Adhesion to the intestinal tract can play a role, but only 10% of the heat-inactivated lactic acid bacteria cells adhere to the intestinal tract, which not only affects the effect of the product, but also increases the production cost

Method used

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  • Inactivated lactic acid bacteria inoculant as well as preparation method and application thereof
  • Inactivated lactic acid bacteria inoculant as well as preparation method and application thereof
  • Inactivated lactic acid bacteria inoculant as well as preparation method and application thereof

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Embodiment

[0084] The cultivation method of Lactobacillus paracasei HYZQF3DS-2 and Lactobacillus plantarum HYZQF1675-63 is as follows:

[0085] 1. Expansion of strain culture

[0086] Expanded culture of strains stored in a low-temperature refrigerator at -80°C, preparation of medium: 32 grams of isomalto-oligosaccharide, 7 grams of beef extract powder, 1 gram of sodium acetate, 0.5ml of Tween-80, 1 gram of potassium dihydrogen phosphate, tap water 1000ml, adjust the pH to 7 (alkaline adjustment).

[0087] Operation: Add the raw materials such as isomaltooligosaccharide in the above formula into 1000ml of water in the formula, stir for 10 minutes to dissolve, then divide into test tubes, each test tube contains 5ml, and seal with kraft paper with rubber stopper , sterilized in a medical sterilizer at 0.1-0.12 MPa for 20 minutes, and the culture medium is completed.

[0088] Inoculation: Under aseptic conditions (ultra-clean workbench), open the freezing tube of the -80°C low-temperatur...

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Abstract

The invention provides an inactivated lactic acid bacteria inoculant as well as a preparation method and application thereof, and belongs to the technical field of microbial preparations. Two strainsof lactic acid bacteria, namely lactobacillus paracasei HYZQF3DS-2 and lactobacillus plantarum HYZQF1675-63, are obtained through screening and further mutagenesis treatment, the two strains of lacticacid bacteria are very suitable for thermal inactivation treatment, and fermentation process research is further carried out on the two strains of lactic acid bacteria, thereby improving the strain fermentation performance; and experiences prove that the microbial inoculum obtained after the fermentation culture of the two strains has good effects of relieving constipation and diarrhea; a meanwhile, the bacteria inoculant has the effects of improving sleep disorder, anxiety and the like, thereby having good practical application value.

Description

technical field [0001] The invention belongs to the technical field of microbial preparations, in particular to an inactivated lactic acid bacteria bacterial preparation and its preparation method and application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] There are a huge number and variety of microorganisms in the human body, which together constitute the human micro-ecosystem. After a long process of biological evolution, the normal flora and the human body are in a symbiotic state, and they have established a close relationship with the human body, which is very important for promoting human physiological functions. The improvement of immune function, especial...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P1/10A61P1/12A61P25/20A61P25/22C12R1/25C12R1/225
CPCC12N1/20A23L33/135A61K35/747A61P1/10A61P1/12A61P25/20A61P25/22A23V2002/00A23V2400/165A23V2400/169A23V2200/32A23V2200/322
Inventor 时敏秀曹崇仁张起凡李瑶
Owner 山东环亿生物科技有限公司
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