Composite microbial agent for preventing and treating soil-borne diseases and preparation method and application thereof

A compound microbial inoculum and soil-borne disease technology, applied in the field of microorganisms, can solve the problems of single disease control, poor comprehensive effect, unstable effect, etc., and achieve the effect of simple process, maintaining ecological balance, and improving control effect.

Inactive Publication Date: 2020-07-10
SHANDONG BEE LAN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventors have found that using a single bio-control bacterium to prevent and control diseases and insect pests not only p

Method used

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  • Composite microbial agent for preventing and treating soil-borne diseases and preparation method and application thereof
  • Composite microbial agent for preventing and treating soil-borne diseases and preparation method and application thereof
  • Composite microbial agent for preventing and treating soil-borne diseases and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0053] In another specific embodiment of the present invention, a preparation method of the above-mentioned composite microbial inoculum is provided, and the preparation method includes:

[0054] Mixing the original bacterial powder of Paecilomyces lilacinus and Bacillus polymyxa evenly to obtain a composite original bacterial powder;

[0055] Mix the adsorption mixture and the composite original bacteria powder evenly to obtain a mixture of microorganisms;

[0056] The mixture for adsorbing microorganisms and the synergistic mixture are uniformly mixed to obtain the composite microbial agent.

[0057] In another specific embodiment of the present invention, the original bacteria powder of Paecilomyces lilacinus and Bacillus polymyxa are obtained by fermentation culture.

[0058] In another specific embodiment of the present invention, the application of the above-mentioned composite microbial inoculants in the prevention and control of soil-borne diseases and the promotion of plant gro...

Example Embodiment

[0063] Example 1 Preparation method of original bacteria powder of Paecilomyces lilacinus and Bacillus polymyxa

[0064] The preparation method of described Paecilomyces lilacinus comprises the following steps:

[0065] Step 1. Cultivation of slant strains. The original strains are inoculated into the PDA slant, and the inoculated slant medium is placed in a 28°C incubator for 14 days to obtain the first batch of slant strains.

[0066] Step 2. Prepare secondary liquid strains, each 1L liquid medium contains: 3g NaNO 3 ,1g K 2 HPO 4 , 0.5gMgSO 4 ·7H 2 O, 0.5g KCl, 0.01g FeSO 4 , 30g sucrose and distilled water. Put the sterilized liquid culture medium into a 3L Erlenmeyer flask, and each bottle is filled with 0.7-0.8L liquid culture medium. Under aseptic conditions, each bottle is inoculated with one slant strain prepared in step 1. After inoculation The liquid culture medium is placed on a shaker for shaking culture for 24-48 hours, at a temperature of 28° C., and a rotation speed ...

Example Embodiment

[0073] Example 2

[0074] The composite microbial agent for preventing and controlling soil-borne diseases in this embodiment is composed of the following parts by weight: 1.5 parts of Paecilomyces lilacinus, 1.0 part of Bacillus polymyxa, 4.2 parts of calcium lignosulfonate, and carboxymethyl 4.2 parts of sodium cellulose, 10.4 parts of tobacco foam, 8.4 parts of auxiliary powder, 6.0 parts of starch, 8.4 parts of diatomaceous earth, 14.5 parts of composite synergist, 11.2 parts of attapulgite, and 30.2 parts of water.

[0075] The compound synergist includes alginic acid, humic acid, and chitin, and the mass ratio of the three is 1:1.5:1.

[0076] In the preparation method of the composite microbial inoculum in this embodiment, the steps are as follows:

[0077] 1. The preparation of the original bacterial powder is the same as in Example 1;

[0078] 2. Mix and stir the original bacteria powder of Paecilomyces lilacinus and Bacillus polymyxa to obtain a composite original bacteria po...

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Abstract

The invention provides a composite microbial agent and a preparation method and application thereof, and belongs to the technical field of microorganisms. The composite microbial agent is prepared from the following components in parts by weight: 1-2 parts of paecilomyces lilacinus, 0.5-1.5 parts of paenibacillus polymyxa, 3-10 parts of calcium lignosulphonate, 2-8 parts of sodium carboxymethyl cellulose, 7-17 parts of tobacco powder, 8-15 parts of auxiliary beauty powder, 3-7 parts of starch, 8-16 parts of a composite synergist, 5-15 parts of diatomite, 7-14 parts of attapulgite and 23.5-35.5parts of water. Paecilomyces lilacinus and paenibacillus polymyxa are combined for use, soil-borne diseases and insect pests such as root-knot nematode, Chinese cabbage soft rot, pepper root rot, tomato seedling blight and pepper phytophthora blight can be effectively prevented and treated, and the product is non-toxic, pollution-free, safe and efficient and is beneficial to maintaining ecological balance and is an effective substitute for part of pesticides abd meets the requirements of agricultural sustainable development and has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a compound microbial bacterial agent for preventing and controlling soil-borne diseases, a preparation method and application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Soil-borne diseases refer to diseases caused by pathogens such as fungi, bacteria, nematodes and viruses that live in the soil with diseased residues and attack crops from roots or stems when conditions are suitable. Chinese cabbage soft rot, pepper root rot, tomato blight and pepper blight are all soil-borne diseases, which have the characteristics of short disease cycle and hi...

Claims

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Application Information

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IPC IPC(8): A01N63/30A01N63/22A01N43/16A01N61/00A01P5/00A01P3/00C12N1/20C12N1/14C12R1/01C12R1/79
CPCA01N43/16A01N61/00A01N63/00C12N1/14C12N1/20
Inventor 单宝龙胡著然王丽荣张文娟齐素敏陈丹丹冉新炎韩广泉陶宁李圆圆黄悦谷巍程福亮徐海燕申小冉徐曙光李敏
Owner SHANDONG BEE LAN BIOTECHNOLOGY CO LTD
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