The invention relates to the technical field of agriculture. The invention provides a cold-resistant flooding-resistant seed coating agent for direct seeding rice. The cold-resistant flooding-resistant seed coating agent comprises a cold-resistant active component, a penetrant, an oxygen producer, an insecticidal bactericide, a trace element fertilizer, a plant growth regulator, an adhesive and anatural pigment. The invention also provides a preparation method of the cold-resistant flooding-resistant direct seeding rice seed coating agent. The preparation method comprises the following steps:accurately weighing the cold-resistant active component, the penetrant, the oxygen producer, the insecticidal bactericide, the trace element fertilizer and the plant growth regulator in proportion, conducting uniform stirring to obtain a mixture, grinding the mixture, weighing the adhesive and the natural pigment, adding the adhesive and the natural pigment into the mixture, conducting uniform stirring, performing quantitative packaging and carrying out hermetic storing. According to the invention, the cold resistance and flooding resistance of the direct seeding rice can be improved, seedling quality is improved, seedlings and roots are strengthened, the use amount of the agent is small, components are easy to obtain, cost is low, and the preparation method is simple.