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Method for producing trichoderma sp. biological control agent from potato starch waste water and mushroom residues

A technology of potato starch and biocontrol agent, applied in the field of microbial pesticide and environmental protection

Inactive Publication Date: 2012-08-01
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the method for producing Trichoderma with potato starch wastewater and mushroom dregs as substratum has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Production of Trichoderma viride biocontrol agent by liquid-solid joint fermentation of potato starch wastewater and mushroom dregs:

[0013] Seed preparation: Sterilize potato starch wastewater at 115°C for 20 minutes, inoculate Trichoderma viride after cooling, at an inoculation concentration of 10 7 spores per milliliter of culture medium, cultured at 26°C and shaken at 150 rpm for 36 hours to obtain liquid seeds.

[0014] Liquid fermentation: Sterilize potato starch wastewater at 115°C for 20 minutes, inoculate 5% liquid seeds, and culture in a fermenter at 26°C for 36 hours with aerobic ventilation to obtain liquid Trichoderma viride product.

[0015] Solid-state fermentation: Using mushroom dregs as a support, add the cultured liquid seeds or liquid Trichoderma products to sugar beet dregs according to 3 times the weight, stir evenly, and culture statically at 26°C for 5 days to obtain solid Trichoderma viride products .

[0016] Post-processing: The solid Trich...

Embodiment 2

[0018] Production of Trichoderma harzianum biocontrol agent by combined liquid-solid fermentation of potato starch wastewater and mushroom dregs:

[0019] Seed preparation: Sterilize potato starch wastewater at 121°C for 15 minutes, inoculate Trichoderma harzianum after cooling, at an inoculation concentration of 10 6 spores per milliliter of culture medium, cultured at 28°C, shake flasks at 150 rpm for 48 hours to obtain liquid seeds.

[0020] Liquid fermentation: Sterilize potato starch wastewater at 121°C for 15 minutes, inoculate 10% liquid seeds, and culture in a fermenter at 28°C for 36 hours with aerobic ventilation to obtain liquid Trichoderma harzianum product.

[0021] Solid-state fermentation: Using mushroom residue as a support, add the cultured liquid seeds or liquid Trichoderma products to bagasse according to 3 times the weight, stir evenly, and culture at 28°C for 7 days with static ventilation to obtain solid-state Trichoderma harzianum product.

[0022] Pos...

Embodiment 3

[0024] Production of Trichoderma chrysanthemum biocontrol agent by liquid-solid joint fermentation of potato starch wastewater and mushroom dregs:

[0025] Seed preparation: Sterilize potato starch wastewater at 115°C for 20 minutes, inoculate Trichoderma chrysogenum after cooling, at an inoculation concentration of 10 6 spores per milliliter of culture medium, cultured at 28°C, shake flasks at 150 rpm for 48 hours to obtain liquid seeds.

[0026] Liquid fermentation: Sterilize potato starch wastewater at 115°C for 20 minutes, inoculate 10% liquid seeds, and culture in a fermenter at 28°C for 36 hours with aerobic ventilation to obtain liquid Trichoderma chrysanthemum products.

[0027] Solid-state fermentation: Using mushroom residue as a support, add the cultured liquid seeds or liquid Trichoderma products to bagasse according to 3.5 times the weight, stir evenly, and culture in static ventilation at 28°C for 8 days to obtain solid Trichoderma chrysanthemum products .

[0...

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PUM

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Abstract

The invention relates to a method for recyclingly producing a trichoderma sp. biological control agent from potato starch waste water and mushroom residues and belongs to the technical field of microbial pesticides and environmental protection. The method adopts high-concentration organic wastewater produced in potato starch production and mushroom residues as main medium raw materials and utilizes a liquid state-solid state combined fermentation production method to produce the trichoderma sp. biological control agent. The method comprises the following steps of 1, inoculating potato starch wastewater as a culture medium with a trichoderma sp. strain and carrying out liquid-state culture to obtain a liquid strain, 2, inoculating potato starch waste water as a fermentation raw material with the liquid strain to obtain a large number of liquid trichoderma sp. products, 3, adding the cultured liquid strain or the liquid trichoderma sp. products into mushroom residues, and carrying out solid state fermentation to obtain solid trichoderma sp. products, and 4, carrying out drying, crushing and packaging of the solid trichoderma sp. products. The method for producing trichoderma sp. biological control agent has the characteristics of low production cost and good environmental benefits. The trichoderma sp. biological control agent obtained by the method can control a plurality of fungal diseases of plants. The method provides an effective treatment approach for high-efficiency treatment on potato starch wastewater and mushroom residues, and reduces environmental pollution.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides and environmental protection. technical background [0002] Refined potato starch can increase in value by about 10-30 times after processing at different depths. However, the current processing technology produces about 6-10 tons of high-concentration organic wastewater for every ton of potato starch produced. The COD value of this wastewater is as high as 10,000 mg / L or more, mainly containing soluble starch, protein, pectin, etc., and is easy to corrupt Fermentation makes the water black and smelly, causing serious pollution. However, the use of general sewage treatment processes to treat such high-concentration wastewater up to the standard requires staggering energy consumption. From another point of view, the organic matter in potato starch wastewater (including: soluble starch, protein, pectin, etc.) is a good nutrient substrate for microbial growth, which can be made into mi...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P3/00C05G3/02C05G3/04C02F3/02C05G3/60C05G3/80
CPCY02W10/10
Inventor 白志辉李宝聚安晓宇王斌科李林杨建州
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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