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Method for producing biological organic fertilizer by using methane liquid as basic culture medium through fermentation

A technology of bio-organic fertilizer and basal medium, which is applied in the field of fermenting bio-organic fertilizer based on biogas slurry as a basal medium, which can solve the problems of limited application and promotion, high price of biological pesticides, and high production costs

Inactive Publication Date: 2011-10-19
SHIHEZI UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the fermentation production of biopesticides at home and abroad usually uses oil residue, soybean meal, corn flour, glucose and nutrients such as inorganic salts and trace amino acids, and the response surface optimization design method is used to optimize the fermentation medium. However, the production of biopesticides with these nutrients The production cost of biopesticides is still relatively high, which leads to high prices of biopesticides and limits their application and promotion.

Method used

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  • Method for producing biological organic fertilizer by using methane liquid as basic culture medium through fermentation
  • Method for producing biological organic fertilizer by using methane liquid as basic culture medium through fermentation
  • Method for producing biological organic fertilizer by using methane liquid as basic culture medium through fermentation

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Embodiment 1

[0053] Embodiment 1: A kind of method that takes biogas slurry as base medium fermentation to produce bio-organic fertilizer is characterized in that mainly comprising the following method steps:

[0054] (1) Preparation of fermentation medium: every 1000mL of biogas slurry is mixed with the following components by weight to obtain a fermentation medium: 10g of corn flour, 10g of soybean meal, 1g of potassium dihydrogen phosphate, 15g of calcium carbonate, sulfuric acid Magnesium 0.5g, Ammonium Sulfate 1g, Glycine 0.5g, Sodium Chloride 1.5g, Potassium Nitrate 2g, sterilize the above fermentation medium for later use, sterilization conditions: 0.15Mpa, 121°C steam sterilization for 30min;

[0055] (2) Streak the Bacillus subtilis preserved at low temperature and glycerol on the slant of the NA nutrient agar medium test tube, and culture it in a 28°C incubator for 24 hours to obtain the spare slant strains. The NA nutrient agar medium includes Prepared from the following compone...

Embodiment 2

[0061] Embodiment 2: Compared with Embodiment 1, this embodiment differs in that:

[0062] The method step (1) is: preparation of fermentation medium: every 1000mL of biogas slurry is mixed with the following components by weight to form a liquid to obtain the fermentation medium: 15g of corn flour, 10g of soybean meal, 2g of potassium dihydrogen phosphate, calcium carbonate 20g, 1g of magnesium sulfate, 1.5g of ammonium sulfate, 0.5g of glycine, 1.5g of sodium chloride, and 4g of potassium nitrate. Sterilize the above fermentation medium for later use. Sterilization conditions: 0.15Mpa, steam sterilization at 121°C for 30min;

[0063] Method step (6) is: add standby fermentation medium in 1000L stirring fermenter, the filling volume of fermentation medium is 600L, 0.10% (w / v) antifoaming agent, by the insertion amount of 60L namely 10 % Connect the third-grade seed liquid, under the conditions of 30°C ventilation rate 10L / min, tank pressure 80kPa, stirring speed 220r / min, and...

Embodiment 3

[0065] Embodiment 3: Compared with Embodiment 1, the present embodiment differs in that:

[0066] The method step (1) is: preparation of fermentation medium: every 1000mL of biogas slurry is mixed with the following components by weight to form a liquid to obtain the fermentation medium: 15g of corn flour, 15g of soybean meal, 2g of potassium dihydrogen phosphate, calcium carbonate 20g, 1g of magnesium sulfate, 1.5g of ammonium sulfate, 0.5g of glycine, 1.5g of sodium chloride, and 4g of potassium nitrate. Sterilize the above fermentation medium for later use. Sterilization conditions: 0.15Mpa, steam sterilization at 121°C for 30min;

[0067] Method step (6) is: add standby fermentation medium in 1000L stirring fermenter, the filling volume of fermentation medium is 600L, 0.10% (w / v) antifoaming agent, by the insertion amount of 60L namely 10 % Insert the third-grade seed liquid, under the conditions of 30°C ventilation rate 10L / min, tank pressure 80kPa, stirring speed 200r / mi...

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Abstract

The invention discloses a method for producing biological organic fertilizer by using methane liquid as a basic culture medium through fermentation. The method mainly comprises the following steps of: (1) preparing a fermentation medium; (2) preparing inclined bacterial strains of bacillus subtilis; (3) performing shaking culture on the inclined bacterial strains to obtain first-level seed liquid; (4) introducing the first-level seed liquid into a culture medium with SDAY to perform culture to obtain second-level seed liquid; (5) performing seed tank culture to obtain third-level seed liquid;and (6) introducing the obtained third-level seed liquid into a fermenting tank to perform amplification culture to obtain the biological organic fertilizer. In the method, the Xinjiang methane fermentation product methane liquid serves as the culture medium; and the organic fertilizer produced by fermenting in the culture medium can control soil-borne disease of commercial crops mainly comprising tomatoes and cotton, can promote growth of the economic crops, has low production cost and good effect, is applicable in Xinjiang, and is economic and practical.

Description

technical field [0001] The invention relates to a method for fermenting and producing bio-organic fertilizer using biogas slurry as a base medium, especially a method using Bacillus subtilis ( Bacillus subtilis ), as a functional microorganism, can not only control the soil-borne diseases of economic crops such as processed tomato and cotton, but also can promote the production method of bio-organic fertilizer for the growth of processed tomato and cotton, and belongs to the field of microbial technology. Background technique [0002] Xinjiang is my country's largest cotton-producing area and the production base of high-quality commercial cotton. However, after entering the 1990s, the proportion of cotton planted area in Xinjiang was too high, and the continuous cropping time was long. After 5-10 years of continuous cropping of cotton, large-scale outbreaks of diseases and insect pests such as cotton bollworm, cotton blight, and verticillium wilt are caused, and continuous c...

Claims

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Application Information

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IPC IPC(8): C05F5/00
CPCY02A40/20
Inventor 赵思峰杜娟张学坤任娟鲁建江刘勇杰李俊华
Owner SHIHEZI UNIVERSITY
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