Construction method of trichoderma-rape combined adsorption of heavy metal cadmium and zinc in farmland soil

A technology for adsorbing heavy metals and Trichoderma, applied in botany equipment and methods, methods based on microorganisms, restoration of contaminated soil, etc., can solve the problems of preventing and controlling soil-borne diseases of rapeseed and subsequent crops, etc. Achieve the effects of improving the efficiency of adsorbing heavy metals, promoting the growth of rapeseed, and controlling soil-borne diseases

Inactive Publication Date: 2011-12-14
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

[0004] After searching the prior art, it was found that there were only individual reports on promoting the adsorption of heavy metals in Brassica juncea by Trichoderma. Lixiang Cao et al. ) after seeding, increase the seedling stems to enrich Ni up to 24-30%, and also increase the enrichment of Cd to a certain extent, and the enrichment of Cd and Ni in roots increases by 8% and 7% (Lixiang Cao, Min Jiang, Zhirui Zen

Method used

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  • Construction method of trichoderma-rape combined adsorption of heavy metal cadmium and zinc in farmland soil
  • Construction method of trichoderma-rape combined adsorption of heavy metal cadmium and zinc in farmland soil
  • Construction method of trichoderma-rape combined adsorption of heavy metal cadmium and zinc in farmland soil

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Experimental program
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Effect test

Embodiment 1

[0024] Step 1: Put different varieties of rapeseeds in a petri dish covered with moist filter paper, 50 seeds per dish, and germinate for 2-3 days under dark conditions at 28°C. The test soil (passed through an 8-mesh sieve) was mixed with ZnSO4·7H2O and CdSO4·8H2O solutions respectively and prepared into four treatments: ZnS04·7H2O was 500 and 1000mg·kg -1 , CdSO4·8H2O is 50, 100mg·kg -1 . The soil treated with heavy metals is stable for 1 week at room temperature. Then the Trichoderma (Trichoderma fermentation broth 1000mL, diatomaceous earth: 500-600g, refined bran: 250-300g, rice straw powder: 80-100g, total fermentation for 5-7 days). Trichoderma powder is mixed with the treated soil at a ratio of 1:20 (w / w), sowed, and cultivated in an artificial climate chamber. Each pot contains 400g soil, and each treatment is repeated 3 times. Twenty days after the rape seedlings emerge, 6 seedlings will be left in each pot. Tap water is used to irrigate the rapeseed during growth. ...

Embodiment 1

[0026] Example 1 Results

[0027] The relative biomass of above ground and purification rate were used to screen for Zn 2+ Or Cd 2+ Rape varieties with obvious enrichment capacity. The data is shown in two kinds of Zn 2+ Under treatment concentration conditions, rapeseed shoots absorb Zn 2+ The most capable variety is Shanghai Oil 20. Huyou 20 in Zn 2+ The content is 500mg·kg -1 And 1000mg·kg -1 The soil purification rate under the soil was 1.32% and 0.85% respectively. In two Cd 2+ Under the treatment concentration conditions, the rapeseed variety with the strongest Cd absorption capacity is Huyou 20. Shanghai Oil 20 in Cd 2+ The content is 50mg·kg -1 And 100mg·kg -1 The soil purification rate under the soil was 1.02% and 0.62%, respectively. By comparing different rape varieties to Zn 2+ And Cd 2+ The purification rate of contaminated soil can be obtained. 2+ , Cd 2+ The purification rate of contaminated soil is the best (Table 1, Table 2).

[0028] Table 1 Relative biomass an...

Embodiment 2

[0034] Step one, inoculate the Trichoderma on the PDA plate, after growing for 2 days, use a 0.5cm diameter puncher on the edge of the colony, and attach the plate to 15mL PDA, containing Cd 2+ 1600mg / L plate, three replicates for each treatment. The colony diameter was measured by the cross method after 5 days.

[0035] Step two, inoculate the silica gel particles of Trichoderma on the PDA plate. After the sporulation, wash the spores with sterile water and count them with a hemocytometer to make the final concentration of spores reach 10 7 Pieces / mL.

[0036] Take sterile water to wash the spores, the final concentration reaches 10 7 Pieces / mL, take 0.5mL spore suspension and inoculate 50mL PD in a 100mL Erlenmeyer flask. Cd 2+ The final concentration was 100mg / L, 28°C and 130rpm shaking culture for 3d. Centrifuge 1mL culture medium at 10000rpm for 10min, and take 30μl supernatant to determine Cd 2+ Concentration

[0037] The determination of Cd concentration refers to the method...

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Abstract

A construction method for antagonistic Trichoderma-Brassica napus joint adsorption of heavy metals cadmium and zinc in farmland soil in the field of metal adsorption technology, by inoculating bacterial agents prepared by adsorbing heavy genus antagonistic Trichoderma strains to bacteria containing Cd or Zn and Sclerotinia sclerotiorum The soil of the wheat grain culture is further used to cultivate Brassica napus and obtain the Trichoderma-rape symbiont with high efficient adsorption of heavy metals. The invention realizes the dual purposes of bioremediation of heavy metal pollution in farmland soil and treatment of soil-borne diseases, has good repair effect, low cost, is easy to manage and operate, and does not produce secondary pollution, so it has high universal use value in the field.

Description

Technical field [0001] The invention relates to a method in the technical field of metal adsorption, in particular to a construction method for antagonizing Trichoderma-Brassica napus to jointly adsorb heavy metals cadmium and zinc in farmland soil. Background technique [0002] Excessive pollution of heavy metals in farmland soil is a source of obstacles to the safe production of agricultural products, which is very harmful to human or animal health. In the early 1990s, by investigating heavy metal pollutants in farmland soil that accounted for 50.5% of the vegetable farming area in Shanghai suburbs, the accumulation of heavy metal pollutants in the soil of Shanghai's vegetable farmland in the past 20 years and its accumulation reasons were analyzed. The results show that Cd, Hg and Zn are the main heavy metal accumulation pollutants in the soil of Shanghai vegetable farmland. In order to reduce soil pollution caused by heavy metals, scientists have proposed physical and chemic...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01G1/00B09C1/10B09C1/00A01N63/04A01P3/00C12R1/885
Inventor 陈捷高永东王秉丽李广记张婷
Owner SHANGHAI JIAO TONG UNIV
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