Compound fungicide comprising Trichoderma asperellum spore powder of and application thereof
A compound fungicide, Trichoderma aculeatus technology, applied in the direction of fungicides, applications, biocides, etc., can solve the problems of excessive residues, large amount of chemical pesticide control, etc., to achieve long-lasting, quick-acting, The effect of using less
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Embodiment 1
[0021] The cultivation and screening of embodiment 1 heat-resistant Trichoderma aculeatus spore powder
[0022] For the general regulations on the heat storage stability of wettable powders, FAO requires storage at (54±2)°C for 14 days or more, but when the preparation is neither suitable nor intended to be used in hot climate conditions, and high temperature has negative effects on the preparation When it is affected, you can change the test conditions, change the conditions (45±2)°C for 6 weeks or more, (40±2)°C for 8 weeks or more, (35±2)°C for 12 weeks or more, (30±2)°C for 12 weeks or more, (30±2)°C )°C for 18 weeks or more. Therefore, for the compound fungicide containing Trichoderma aculeatus spore powder, there are higher requirements on the adaptable heat storage temperature and heat storage time of the Trichoderma aculeatus spore powder.
[0023] 1) heat-storing the first-generation Trichoderma aculeatus spore powder at a temperature of 45° C. and 54° C. for 30 days...
Embodiment 2
[0031] The screening of embodiment 2 chemical fungicides
[0032] (1) Inhibitory effect of different fungicides on the growth of Trichoderma mycelium
[0033] The inhibitory effect of different fungicides on the growth of Trichoderma aculeatus mycelium was measured by the method of mycelial growth rate, and the chemical fungicides that could be compounded with Trichoderma aculeatus spore powder were screened out.
[0034] Method: The growth inhibition of Trichoderma aculeatus mycelia within the registered dosage range of each agent was determined. Firstly, each agent is prepared into a certain concentration mother solution, and the required volume is drawn according to the set concentration, and then added to the triangular flask. The triangular flask contains melted PDA medium, which is usually added at 45°C, mixed evenly and poured into sterilized petri dishes respectively. In order to make the observation more durable, a 12cm petri dish is selected. Trichoderma cultured i...
Embodiment 3
[0051] The screening of embodiment 3 surfactants
[0052] Surfactants with little effect on the activity of Trichoderma aculeatus spore powder were screened out. First, the surfactant after the air powder is mixed with Trichoderma aculeatus in a certain proportion, stored at room temperature, and the live spore rate of each treatment Trichoderma aculeatus is measured after 7 days, 30 days, 60 days, and half a year. Trichoderma aculeatus spore powder with any surfactant was used as a control.
[0053] Table 4 Surfactant Screening
[0054]
[0055] The results show that in dispersants, lignin sulfonate, naphthalene sulfonate, polycarboxylate, polynaphthalene sulfonate, anion-nonionic complex agent, in wetting agent, alkylbenzene sulfonate, alkane Sodium sulfate and sulfates have the least effect on the activity of Trichoderma spore powder.
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