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Preparation and application of a strain of Paenibacillus polymyxa and its bacterial agent

A technology of Paenibacillus polymyxa and bacillus, which is applied in the field of preparation of Paenibacillus polymyxa and its bacterial agent, can solve the problems of micro-controlling effect, achieve the effects of increasing soil nutrient elements, increasing the yield of Salvia miltiorrhiza, and promoting the growth of Salvia miltiorrhiza

Active Publication Date: 2022-07-05
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For the prevention and control of root rot of Salvia miltiorrhiza, currently chemical pesticides are mainly used for control, but the control effect is very small; and the use of beneficial microbial agents to control fungal diseases of Salvia miltiorrhiza is the best choice for solving soil-borne diseases of Chinese medicinal materials

Method used

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  • Preparation and application of a strain of Paenibacillus polymyxa and its bacterial agent
  • Preparation and application of a strain of Paenibacillus polymyxa and its bacterial agent
  • Preparation and application of a strain of Paenibacillus polymyxa and its bacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1. Isolation of strain DS-R5

[0047] Take healthy Salvia miltiorrhiza plants with good growth, wash them with running water, and disinfect the surface of the material according to the following process: soak in 75% ethanol for 1 min and rinse with sterile water for 3 times; soak in 0.1% mercuric chloride for 3 minutes and rinse with sterile water for 3 times. The treated tissues such as roots, stems, and leaves of Salvia miltiorrhiza were ground into a slurry in a sterile operating table, diluted 10 times with sterile water, and then coated on NA medium, and cultured at 37°C for 3 days. When visible colonies grow on the medium, the colonies are picked in time, and pure cultures are obtained by streaking multiple times.

[0048] Using Fusarium solani as the target pathogen, the endogenous antagonistic bacteria of Salvia miltiorrhiza were screened. Using the two-point plate confrontation method, the isolated Salvia miltiorrhiza endophytic bacteria were tested for pathog...

Embodiment 2

[0060] Example 2: Determination of Bacteriostatic Spectrum of Strain DS-R5 on Common Plant Pathogenic Fungi

[0061] The isolated endophytic bacteria DS-R5 of Salvia miltiorrhiza were tested for their antagonistic performance against pathogenic fungi by the two-point plate confrontation method. Take Fusarium rot, Rhizoctonia solani, Alternaria, Fusarium graminearum, Fusarium oxysporum, Fusarium pseudograss, anthracnose, and Vitis vinifera that have been cultured for 7 days, and use a 5mm diameter The hole was punched, and a 5mm bacterial cake was inoculated in the center of the PDA plate, and the isolated Salvia miltiorrhiza endophytic bacteria were inoculated at a distance of 2.5 cm from the bacterial cake. nourish. When the pathogenic bacteria colonies in the control group covered the bottom of the dish, the width of the inhibition zone in the treatment group was measured, and each treatment was repeated three times. The test results show that the strain DS-R5 has differen...

Embodiment 3

[0065] Example 3: Determination of Bacteriostatic Spectrum of Strain DS-R5 on Common Pathogenic Bacteria

[0066] Take a strain of Staphylococcus aureus, Escherichia coli, Candida albicans, septicemia nigra, Candida albicans, and bacterial poplar canker to LB medium for overnight culture, and take 20 μL of the above-mentioned logarithmic phase. The six tested pathogenic bacteria were added to 20 mL of uncoagulated LB medium, mixed and poured into a sterile petri dish with a diameter of 7.5 cm. After coagulation, the strain DS-R5 was seeded with a sterilized toothpick, and the antagonistic effect of the strain DS-R5 on the tested pathogenic bacteria was observed after culturing at 37°C for 24 hours. The test results show that the strain DS-R5 has different degrees of inhibitory effect on the 6 kinds of pathogenic bacteria tested (see Figure 4 ).

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Abstract

The invention discloses the preparation and application of a strain of Paenibacillus polymyxa DS-R5 and a bacterial agent thereof. The strain has been deposited in the China Center for Type Culture Collection on January 4, 2021, and the biological deposit number is: CCTCC M 2021005 . The strain provided by the invention has inhibitory effect on various pathogenic bacteria, especially has strong antagonistic effect on Fusarium solani, Rhizoctonia solani and the like which cause root rot. The strain can not only increase soil nutrients, but also improve the rhizosphere microbial community structure of Salvia miltiorrhiza, inhibit the number of pathogenic bacteria in the soil, thereby interfering with the infection of the roots of Salvia miltiorrhiza by pathogenic bacteria, and has the dual effect of disease prevention and growth promotion. At the same time, the microbial fertilizer with this strain as the active ingredient has low cost, high fertilizer efficiency and no pollution, and is the best substitute for chemical fertilizers and pesticides often used in the prevention and control of Salvia miltiorrhiza root rot; Diseases and improving the yield of Salvia miltiorrhiza have guiding significance.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to the preparation and application of a strain of Paenibacillus polymyxa and a bacterial agent thereof. Background technique [0002] Salvia miltiorrhiza (Salvia miltiorrhiza) is a perennial herbal medicinal plant, and its roots are used as medicine. Salvia miltiorrhiza is one of the medicinal materials with a large cultivated area. Under artificial cultivation conditions, due to the poor diversity and low abundance of biological communities in the planting ecological environment, the dominant populations of diseases and insect pests are prominent, and serious diseases and insect pests often occur, affecting the growth and development of Salvia miltiorrhiza plants. As a result, the yield of medicinal materials decreased and the quality deteriorated. [0003] The root rot of Salvia miltiorrhiza is a typical fungal infectious disease. The spores of the pathogenic ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/25A01P3/00A01P1/00A01P21/00C05G3/60C12R1/01
CPCC12N1/20A01N63/25C05G3/60
Inventor 洒荣波宋枚枚许乐王子强张爽邢倩刘丽娜
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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