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Method for producing Trichoderma biocontrol agent by utilizing sweet potato starch waste water and mushroom dregs

A technology for sweet potato starch wastewater and mushroom residue, which is applied in the fields of microbial pesticides and environmental protection

Inactive Publication Date: 2012-07-04
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the method of producing Trichoderma with sweet potato starch waste water and mushroom slag as substratum has not been reported yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Trichoderma viride biocontrol agent produced by liquid-solid joint fermentation of sweet potato starch wastewater and mushroom residue:

[0013] Seed preparation: Sterilize sweet potato starch wastewater at 115°C for 20 minutes, inoculate Trichoderma viride after cooling, at an inoculation concentration of 10 7 spores per milliliter of culture medium, cultured at 26°C and shaken at 150 rpm for 36 hours to obtain liquid seeds.

[0014] Liquid fermentation: Sterilize sweet potato starch wastewater at 115°C for 20 minutes, inoculate 5% liquid seeds, and culture in a fermenter at 26°C for 36 hours with aerobic ventilation to obtain liquid Trichoderma viride product.

[0015] Solid-state fermentation: Using mushroom dregs as a support, add the cultured liquid seeds or liquid Trichoderma products to sugar beet dregs according to 3 times the weight, stir evenly, and culture statically at 26°C for 5 days to obtain solid Trichoderma viride products .

[0016] Post-processing: ...

Embodiment 2

[0018] Production of Trichoderma harzianum biocontrol agent by combined liquid-solid fermentation of sweet potato starch wastewater and mushroom dregs:

[0019] Seed preparation: Sterilize sweet potato starch wastewater at 121°C for 15 minutes, inoculate Trichoderma harzianum after cooling, at an inoculation concentration of 10 6 spores per milliliter of culture medium, cultured at 28°C, shake flasks at 150 rpm for 48 hours to obtain liquid seeds.

[0020] Liquid fermentation: Sterilize sweet potato starch wastewater at 121°C for 15 minutes, inoculate 10% liquid seeds, and culture in a fermenter at 28°C for 36 hours with aerobic ventilation to obtain liquid Trichoderma harzianum product.

[0021] Solid-state fermentation: Using mushroom residue as a support, add the cultured liquid seeds or liquid Trichoderma products to bagasse according to 3 times the weight, stir evenly, and culture at 28°C for 7 days with static ventilation to obtain solid-state Trichoderma harzianum prod...

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Abstract

The invention relates to a method for producing a trichoderma biocontrol agent by taking sweet potato starch waste water and mushroom dregs as main raw materials, and belongs to the technical field of microbial pesticide and environmental protection. Trichoderma strains are the conventional farm-oriented biocontrol strains, such as trichoderma viride, trichoderma harziamum and trichoderma auxeoviride. The method for producing the trichoderma biocontrol agent comprises the following four steps of: preparing seeds, performing liquid fermentation, performing solid state fermentation and performing aftertreatment. According to the method, a solid microbial inoculum is prepared by taking the sweet potato starch waste water as a main carbon source and water of a fermentation culture medium and the mushroom dregs as a carrier of microbes. In the technical scheme, a new path is provided for the recycling of the high-concentration sweet potato starch waste water, and the produced trichoderma biocontrol agent can be applied in fields and has a good prevention and control effect on fungus diseases of multiple plants.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides and environmental protection. technical background [0002] Sweet potatoes are perishable and should not be stored for a long time. The deep processing of sweet potatoes can solve the phenomenon of a large number of rotten potatoes caused by improper storage of fresh potatoes. The refined starch of sweet potatoes can be processed at different depths to produce hundreds of valuable products, and the value-added is about 10-30 times. However, the current processing technology produces about 6 tons of high-concentration organic wastewater for every ton of sweet potato starch produced. The COD value of this wastewater is as high as 10,000 mg / L, mainly containing soluble starch, protein, pectin, organic acid and a small amount of Grease, perishable and fermented, makes the water black and smelly. Dissolved oxygen in the water will be consumed when discharged into the river, which will pro...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P3/00A01P1/00
Inventor 彭霞薇周巍侯交弟高莎白志辉
Owner BEIJING FORESTRY UNIVERSITY
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