Molecular biomarker for diagnosing and treating triple-negative breast cancer and application thereof

A technology for triple-negative breast cancer and breast cancer cells, which is applied in the fields of crude drug medicine and molecular biology, and can solve the problem that the function and role of circRNA are not fully understood.

Active Publication Date: 2021-03-02
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the function and role of circRNA are still not fully understood

Method used

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  • Molecular biomarker for diagnosing and treating triple-negative breast cancer and application thereof
  • Molecular biomarker for diagnosing and treating triple-negative breast cancer and application thereof
  • Molecular biomarker for diagnosing and treating triple-negative breast cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0147] 1. Sample collection

[0148] Collect 100 pairs of tumor tissues and normal control tissues from patients undergoing breast cancer surgery in Qilu Hospital of Shandong University from 2015 to 2019, and sign consent forms for all patients participating in the study.

[0149] 2. circRNA quantitative PCR experiment:

[0150] 2.1 Extraction of tissue total RNA

[0151] Total RNA was extracted from the tissues of breast cancer patients using Trizol reagent, and the concentration and purity of RNA were detected using corresponding equipment.

[0152] 2.2 Reverse transcription of total RNA

[0153] Reverse transcription was performed using the reverse transcription kit from Takara Company, the initial amount of RNA was 1 μl, and the reverse transcription of RNA was performed according to the instructions provided.

[0154] The system is shown in the table below:

[0155]

[0156] Specific reaction program settings in the PCR instrument:

[0157]

[0158] 2.3circRNA ...

Embodiment 2

[0168] At the same time, the present invention conducts statistics on the expression of circEIF3H in the sampled patients with triple-negative breast cancer, and conducts a comprehensive statistical analysis in combination with its therapeutic effect, such as Figure 4 As shown, it was found that triple-negative breast cancer patients with high expression of circEIF3H had a worse prognosis than triple-negative breast cancer patients with low expression of circEIF3H.

Embodiment 3

[0170] Effects on the growth rate and migration ability of cancer cells.

[0171] 1. The effect of circEIF3H on the growth rate of breast cancer cells

[0172] 1.1 Cell transfection

[0173] Take a 6cm petri dish as an example:

[0174] (1) Select a suitable cell culture dish according to the experimental requirements, count the cells one day before transfection, and plate according to the transfection requirements.

[0175] (2) Before transfection, replace the cell culture medium with an appropriate amount of medium without antibiotics, for example, add 2ml of medium to a 6cm dish.

[0176] (3) Take out the EP tube and mark the name of lipo or transfected siRNA respectively.

[0177] siRNA sequence:

[0178] si-circEIF3H-1: CAGCAGUCCAAUAUCAGAU (SEQ ID No. 6);

[0179] si-circEIF3H-2: CAGCCUUGCCAGCAGUCCA (SEQ ID No. 7).

[0180] (4) According to the ratio recommended in the instruction manual of Lipofectamine2000, take a 6cm dish as an example, take 250μl of opti-MEM to ...

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Abstract

The invention provides a molecular biomarker for diagnosing and treating triple-negative breast cancer and application thereof, and belongs to the technical field of crude drugs and molecular biology.The invention finds for the first time that circEIF3H expression in a tumor specimen of a patient suffering from triple-negative breast cancer is obviously higher than that of breast cancer tissues of other subtypes, meanwhile, the circEIF3H expression quantity is related to prognosis of triple-negative breast cancer, and malignant growth and invasion capacity of breast cancer cells can be controlled by regulating the circEIF3H expression. Therefore, the circEIF3H can be used as a molecular biomarker for diagnosing and treating the triple-negative breast cancer, and can be used as a prognosisindex and a potential treatment target of the triple-negative breast cancer. The biomarker provides a more favorable means for diagnosis and prognosis evaluation analysis of the triple-negative breast cancer, has important significance for research and treatment of the triple-negative breast cancer, and thus, has favorable practical application value.

Description

technical field [0001] The invention belongs to the technical field of crude drug medicine and molecular biology, and specifically relates to a molecular biomarker for diagnosis and treatment of triple-negative breast cancer and its application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Breast cancer is one of the most common malignant tumors worldwide. According to the latest epidemiological statistics in 2015, there are 268,600 new cases of breast cancer and 69,500 breast cancer deaths in my country every year. It is one of the countries with the fastest growth rate of breast cancer incidence and mortality in the world. The age and age of death showed a trend of g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11A61K45/00A61P35/00A61P35/04
CPCC12Q1/6886A61K45/00A61P35/00A61P35/04C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 杨其峰宋肖瑾
Owner SHANDONG UNIV QILU HOSPITAL
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