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A molecular biomarker for the diagnosis and treatment of triple-negative breast cancer and its application

A triple-negative breast cancer and material technology, applied in the fields of herbal medicine and molecular biology, can solve the problems such as the incomplete understanding of the function and role of circRNA

Active Publication Date: 2022-06-24
SHANDONG UNIV QILU HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the function and role of circRNA are still not fully understood

Method used

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  • A molecular biomarker for the diagnosis and treatment of triple-negative breast cancer and its application
  • A molecular biomarker for the diagnosis and treatment of triple-negative breast cancer and its application
  • A molecular biomarker for the diagnosis and treatment of triple-negative breast cancer and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0147] 1. Sample collection

[0148] A total of 100 pairs of tumor tissues and normal control tissues were collected from patients undergoing breast cancer surgery in Qilu Hospital of Shandong University from 2015 to 2019, and all patients who participated in the study signed the love form.

[0149] 2. CircRNA quantitative PCR experiment:

[0150] 2.1 Extraction of total RNA from tissues

[0151] Total RNA was extracted from tissues of breast cancer patients using Trizol reagent, and the concentration and purity of RNA were detected by corresponding equipment.

[0152] 2.2 Reverse transcription of total RNA

[0153] Reverse transcription was performed using the reverse transcription kit of Takara Company, and the initial amount of RNA was 1 μl, and the reverse transcription of RNA was performed according to the provided instructions.

[0154] The system is shown in the following table:

[0155]

[0156] The specific reaction program settings in the PCR machine:

[0157...

Embodiment 2

[0168] At the same time, in the present invention, the expression of circEIF3H in the sampled triple-negative breast cancer patients is counted, and comprehensive statistical analysis is carried out in combination with its therapeutic effect. Figure 4 As shown, it was found that triple-negative breast cancer patients with high expression of circEIF3H had a worse prognosis than triple-negative breast cancer patients with low expression of circEIF3H.

Embodiment 3

[0170] Effects on the growth rate and migration ability of cancer cells.

[0171] 1. The effect of circEIF3H on the growth rate of breast cancer cells

[0172] 1.1 Cell transfection

[0173] Take a 6cm petri dish as an example:

[0174] (1) Select a suitable cell culture dish according to the experimental requirements, count the cells one day before transfection, and plate them according to the needs of transfection.

[0175] (2) Before transfection, change the cell culture medium to an appropriate amount of medium without antibiotics, for example, add 2 ml of medium to a 6 cm dish.

[0176] (3) Take out the EP tube and mark the name of lipo or transfected siRNA respectively.

[0177] siRNA sequence:

[0178] si-circEIF3H-1: CAGCAGUCCAAUAUCAGAU (SEQ ID No. 6);

[0179] si-circEIF3H-2: CAGCCUUGCCAGCAGUCCA (SEQ ID No. 7).

[0180] (4) According to the ratio recommended in the instructions of Lipofectamine2000, taking a 6 cm dish as an example, dissolve 10 μl of liposomes a...

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Abstract

The invention provides a molecular biomarker for diagnosing and treating triple-negative breast cancer and its application, and belongs to the technical fields of herbal medicine and molecular biology. The present invention found for the first time that the expression of circEIF3H in the tumor specimens of patients with triple-negative breast cancer was significantly higher than that of other subtypes of breast cancer tissues, and the expression of circEIF3H was related to the prognosis of triple-negative breast cancer, and could be controlled by regulating the expression of circEIF3H Malignant growth and invasion ability of breast cancer cells. Therefore, circEIF3H can be used as a molecular biomarker for the diagnosis and treatment of triple-negative breast cancer, that is, as a prognostic indicator and a potential therapeutic target for triple-negative breast cancer. The invention provides a more favorable means for the diagnosis and prognosis evaluation analysis of triple-negative breast cancer, has important significance for the research and treatment of triple-negative breast cancer, and thus has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of crude drug medicine and molecular biology, and particularly relates to a molecular biological marker for diagnosis and treatment of triple-negative breast cancer and its application. Background technique [0002] The information disclosed in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] Breast cancer is one of the most common malignant tumors worldwide. The latest epidemiological statistics in 2015 show that there are 268,600 new cases of breast cancer and 69,500 deaths from breast cancer in my country every year. The age and the age of death showed a gradually younger trend. Triple negative breast cancer (TNBC) is a subtype of breast cancer that is negati...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11A61K45/00A61P35/00A61P35/04
CPCC12Q1/6886A61K45/00A61P35/00A61P35/04C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 杨其峰宋肖瑾
Owner SHANDONG UNIV QILU HOSPITAL
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