Method for purifying and preparing poplar wood xylooligosaccharide by biological enzyme together with physical and chemical methods

A technology of physical and chemical methods and xylooligosaccharides, which is applied in the field of purification and preparation of xylooligosaccharides from poplar wood by biological enzymes and physical and chemical methods, which can solve the problems of low yield of xylooligosaccharides, low loss of xylose, and low production costs. Advanced problems, to achieve the effect of increasing xylooligosaccharide content, promoting lignin removal, and reducing energy consumption

Active Publication Date: 2019-07-23
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the deficiencies in the above-mentioned prior art, in order to effectively utilize poplar wood hot water pre-hydrolyzate to prepare high-purity, high-yield xylo-oligosaccharides and realize high-value utilization of the pre-hydrolyzate, the present invention provides a xylose loss rate Low xylooligosaccharide content, high purity prehydrolyzed xylooligosaccharide preparation process to solve the traditional method of using prehydrolyzed xylooligosaccharide preparation process cumbersome, low xylooligosaccharide yield, Problems such as low purity and high production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] After the poplar hot water prehydrolyzate was centrifuged, the supernatant was taken, and xylooligosaccharides were prepared according to the following steps:

[0046] (1) Treat poplar hot water prehydrolyzate with calcium hydroxide under normal temperature conditions. Wherein, the dosage of calcium hydroxide is 0.6wt%, and the treatment time is 30min.

[0047] (2) Centrifuge the hydrolyzed solution treated with calcium hydroxide, and take the supernatant. Centrifugation speed 4500rpm, centrifugation time 5min.

[0048] (3) The supernatant obtained in step (2) is treated with laccase and xylanase at a temperature of 55° C. and a pH value of 5.5. Among them, the dosage of laccase is 1U / g 水解液 , the dosage of xylanase is 2U / g 水解液 , the processing time is 2h.

[0049] (4) The hydrolyzate treated with laccase and xylanase was placed in a boiling water bath for 10 minutes.

[0050] (5) The hydrolyzate after boiling water bath treatment is carried out activated carbon tr...

Embodiment 2

[0054] After the poplar hot water prehydrolyzate was centrifuged, the supernatant was taken, and xylooligosaccharides were prepared according to the following steps:

[0055] (1) Treat poplar hot water prehydrolyzate with calcium hydroxide under normal temperature conditions. Wherein, the dosage of calcium hydroxide is 0.6wt%, and the treatment time is 20min.

[0056] (2) Centrifuge the hydrolyzed solution treated with calcium hydroxide, and take the supernatant. Centrifugation speed 4500rpm, centrifugation time 5min.

[0057] (3) The supernatant obtained in step (2) is treated with laccase and xylanase at a temperature of 50° C. and a pH value of 4.5. Among them, the dosage of laccase is 2U / g 水解液 , the dosage of xylanase is 3U / g 水解液 , the processing time is 3h.

[0058] (4) The hydrolyzed solution treated with laccase and xylanase was placed in a boiling water bath, and treated in the boiling water bath for 10 minutes.

[0059] (5) Treat the hydrolyzed solution after th...

Embodiment 3

[0063] After the poplar hot water prehydrolyzate was centrifuged, the supernatant was taken, and xylooligosaccharides were prepared according to the following steps:

[0064] (1) Treat poplar hot water prehydrolyzate with calcium hydroxide under normal temperature conditions. Wherein, the dosage of calcium hydroxide is 0.8wt%, and the treatment time is 10min.

[0065] (2) Centrifuge the hydrolyzed solution treated with calcium hydroxide, and take the supernatant. Centrifugation speed 4500rpm, centrifugation time 5min.

[0066] (3) The supernatant obtained in step (2) is treated with laccase and xylanase at a temperature of 50° C. and a pH value of 5.0. Among them, the dosage of laccase is 2U / g 水解液 , the dosage of xylanase is 2U / g 水解液 , the processing time is 3h.

[0067] (4) The hydrolyzate treated with laccase and xylanase was placed in a boiling water bath for 10 minutes.

[0068] (5) The hydrolyzate after boiling water bath treatment is carried out activated carbon tr...

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Abstract

The invention discloses a method for purifying and preparing poplar wood xylooligosaccharide by a biological enzyme together with physical and chemical methods, and belongs to the technical field of biological refining. The preparation method simultaneously performs purification of pre-hydrolysis liquor and preparation of xylooligosaccharide, specifically treats the poplar wood pre-hydrolysis liquor by using a physical, chemical and biological combination method including calcium hydroxide treatment, laccase and xylanase treatment and activated carbon treatment; and, and separates and removessoluble impurities such as lignin and furfural in the pre-hydrolysis liquor to the maximum extent under the condition of ensuring high xylooligosaccharide yield and purity. The method solves the problems of long period, high energy consumption, complex process and high production cost in the process of producing xylooligosaccharide by traditional methods, improves the utilization value of the poplar wood pre-hydrolysis liquor, and realizes the high-value utilization of hemicellulose component of broad-leaf wood such as poplar wood. Therefore, the method has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of biological refining, and in particular relates to a method for purifying and preparing poplar xylooligosaccharides with biological enzymes in cooperation with physical and chemical methods. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] In recent years, the market demand for dissolving pulp has been increasing year by year. Prehydrolyzate is increasingly produced as a product of the dissolving pulp production process. In large-scale production, some enterprises usually concentrate and burn the pre-hydrolyzed liquid and black liquor together to generate heat. Due to the high moisture content in the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/00C12P19/04C12P19/12C12P19/14
CPCC12P19/04C12P19/00C12P19/12C12P19/14
Inventor 杨桂花陈嘉川徐丰吉兴香董吉冉
Owner QILU UNIV OF TECH
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