10 results about "Tobacco use" patented technology

This invention relates to the cloning and application of the stress resistance gene LpbHLH75 in *Lilium pumilum* DC. This invention aims to identify key stress resistance genes in *Lilium pumilum* through transcriptome data analysis and to study their functions. The steps include: (1) cloning of the target gene; (2) construction of a plant overexpression vector; and (3) detection of physiological indicators in transgenic plants. This experiment uses *Lilium pumilum* as the research object, cloning the LpbHLH75 gene using RT-PCR technology; transforming tobacco using an overexpression vector; identifying the function of LpbHLH75 overexpression against salt-alkali and drought stress in tobacco through transgenic tobacco, revealing the function of the *Lilium pumilum* bHLH gene under adverse stress, providing a new gene source for lily genetic engineering breeding, providing a reference for future research on lily stress resistance germplasm resources, and laying the foundation for discovering genes in *Lilium pumilum* that can enhance stress resistance and their stress resistance mechanisms.

The invention discloses a method for improving the quality of cigar tobacco leaves by using arginine, which comprises the following steps: spraying moisture regain water containing arginine to the cigar tobacco leaves, and standing to balance the moisture content of the tobacco leaves to 30-35% to obtain moisture regained tobacco leaves; and fermenting the moisture-regained tobacco leaves at the relative humidity of 75-85% to complete quality improvement. According to the method, arginine is added to serve as the quality improving agent, the aroma quality and the overall homogenization level of the cigar tobacco leaves can be synergistically and remarkably improved, and according to the scheme, generation of multiple key aroma substances such as carotenoid degradation products, Maillard reaction products and cembrane degradation products in the tobacco leaves can be efficiently promoted; the distribution uniformity of the aroma in a tobacco pile and the uniformity of the appearance chromaticity (including brightness, redness and yellowness) of tobacco leaves are greatly improved.

The application provides a preparation method of a tobacco use Ampelopsis grossedentata Maillard reactant, comprising the following steps: A) mixing Ampelopsis grossedentata powder with water, repeatedly freezing and thawing, and then performing ultrasonic extraction to obtain an Ampelopsis grossedentata extract; B) performing fermentation on the Ampelopsis grossedentata extract by using high-activity wine yeast to obtain an Ampelopsis grossedentata fermentation liquor; and C) adjusting the pH of the Ampelopsis grossedentata fermentation liquor by using potassium carbonate and then performing a heating reaction to obtain the tobacco use Ampelopsis grossedentata Maillard reactant. The Ampelopsis grossedentata extract is prepared by using ultralow-temperature freezing and thawing combined with ultrasonic waves, and the extraction process is safe and efficient without using any organic solvent; meanwhile, the Ampelopsis grossedentata extract is fermented by using active yeast to generate new flavor components; further, Maillard reaction is performed on the amino acids and reducing sugars of the Ampelopsis grossedentata itself, which can increase the fullness of aroma, improve the delicate softness of smoke, and obviously improve the comfort of cigarettes in the application of cigarettes.

This invention relates to a method and system for tobacco-related analysis based on visual information. The method involves converting and classifying the collected tobacco-related data, and then detecting and identifying tobacco types in tobacco-related images, extracting tobacco-related license plates from tobacco-related license plate images, and identifying and extracting tobacco-related text from tobacco-related text images. This, combined with personnel information and investigation data, enables rapid tobacco-related analysis of the collected data, helping to quickly identify key personnel.

This utility model relates to the field of cable tie technology and discloses an automatic cable tie machine using water-based adhesive for tobacco products. It includes a frame body, with support plates fixedly installed at the bottom of the left and right side walls of the frame body. A conveying mechanism is provided within the inner cavity of each support plate. First support blocks are fixedly installed on the left and right sides of the upper end face of the frame body. A center seat is fixedly installed within the inner cavity of each of the first support blocks on both sides. A lifting mechanism is provided on the lower end face of the center seat. Second support blocks are fixedly installed on the left and right sides of the front and rear of the upper end face of the frame body. A top seat is fixedly installed within the inner cavity of each of the second support blocks on both sides. A sealing mechanism is provided on the lower end face of the top seat. This utility model, through the conveying mechanism, can continuously transport bottles, cans, and packaging bags. Through the lifting mechanism, packaging bags on the conveyor belt can be adsorbed and lifted upwards. Finally, with the cooperation of the sealing mechanism, the packaging bags can be heat-shrink and tightened to achieve the sealing of bottles and cans.

This utility model discloses a real-time online correction device for preparing reconstituted tobacco using the slurry method. The correction device (1) includes a slider (11), a guide rail (12), a cylinder (15), a cylinder connecting push rod (16), and a displacement sensor (17). The cylinder connecting push rod (16) connects the slider (11) and the cylinder (15). The slider (11) is arranged on the guide rail (12), and a bearing (13) is located inside the slider (11). The bearing (13) is fixedly connected to the central axis of the correction roller (14). The guide rail (12) is arranged on the correction mounting frame (22). The correction roller (14) is arranged on the lower surface of the steel strip (3). The correction device of this utility model can achieve real-time online continuous correction during the preparation of reconstituted tobacco, ensuring the stability of the preparation of reconstituted tobacco.

A raw material formulation adjustment method for production of reconstituted tobacco using a rolling process. Raw materials of reconstituted tobacco produced by means of the rolling process comprise a powder system and a liquid system; the method comprises: mixing raw materials of the liquid system to obtain a liquid mixture; measuring the viscosity of the liquid mixture; and, on the basis of the viscosity of the liquid mixture, adjusting parameters of raw materials of the powder system, the parameters of the raw materials of the powder system comprising the particle size of the tobacco raw material and / or the sugar content of the tobacco raw material. The method ensures that the final slurry-like material formed by mixing the powder system and the liquid system has suitable properties, and no roller sticking occurs; in addition, the viscosity of the liquid mixture is controlled within the range of 4000 mPa.s-6000 mPa.s, which ensures that the finally obtained reconstituted tobacco has suitable density, a suitable filling value, and good processability, and can reduce breakage of the reconstituted tobacco during a cutting process. Also provided is the use of the method in production of reconstituted tobacco using the rolling process.

A method for determining tobacco-specific nitrosamines (TSNAs) in tobacco using one-step clean-up coupled with liquid chromatography-tandem mass spectrometry (LC-MS / MS) is provided, including the following steps: destemming flue-cured tobacco or sun-cured tobacco, drying, milling, and passing through a sieve; mixing the resulting tobacco, an internal standards solution and water in a plastic centrifuge tube, and vortexing the resulting mixture at room temperature to allow extraction; transferring an extraction solution after filtration to a new centrifuge tube, adding dichloromethane, and vortexing the resulting mixture; centrifuging to collect a dichloromethane extraction solution in a lower layer to another centrifuge tube, and placing the centrifuge tube in a water bath to remove dichloromethane; dissolving the resulting extraction solution in water, and transferring the resulting solution to an autosampler vial for LC-MS / MS analysis. The method has better separation and sensitivity, simple operations, and wide adaptability.

This invention provides a method and system for grouping flue-cured tobacco using feature channel weighting and dynamic loss control, relating to the fields of deep learning and flue-cured tobacco grading. The method involves acquiring images of flue-cured tobacco from N main groups using an image acquisition device to establish a flue-cured tobacco grouping dataset, where N is a positive integer. This dataset is then preprocessed to obtain a preprocessed dataset. A flue-cured tobacco grouping classification network (TGNet) is designed and trained on the preprocessed dataset to obtain a flue-cured tobacco grouping classification model. Based on this model, the grouping results are obtained. This invention solves the technical problems of existing deep learning methods for flue-cured tobacco grouping, such as lack of key feature representation in high-scale features, limited inter-class discrimination ability, and a tendency for the model to learn from majority class samples during training. It achieves real-time classification of flue-cured tobacco groups, effectively improving the efficiency of flue-cured tobacco group classification and reducing the cost of manual grading.

The invention belongs to the technical field of biological agriculture, and particularly relates to an application of an Nta04g10180 gene and an encoding protein thereof in improving black shank resistance of cultivated tobacco. The resistance of Nta04g10180 gene negative regulation cultivation tobacco to tobacco black shank pathogenic bacterium phytophthora nicotianae is found, and tests prove that silencing of the Nta04g10180 gene down-regulates the expression of SA (salicylic acid) signal channel marker gene NtPR1, activates SA-dependent immune response, enhances active oxygen bursting, and further improves disease resistance; and overexpression of the Nta04g10180 gene enables the tobacco to be more susceptible to diseases. The invention provides a new target for breeding for disease resistance of cultivated tobacco, the disease resistance of the tobacco can be enhanced by knocking out the gene or reducing the expression of the gene in the cultivated tobacco by using gene editing technologies such as CRISPR-Cas9 and the like, and the gene has a wide application prospect in the aspect of improving the disease resistance of the tobacco.