Ulcerative colitis biomarker based on intestinal flora and application of ulcerative colitis biomarker

A biomarker technology for ulcerative colitis, applied in the field of biomedicine, can solve problems such as the inability to predict the onset and development trend, and the inability to diagnose ulcerative colitis

Pending Publication Date: 2020-03-24
SHANDONG UNIV QILU HOSPITAL
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a biomarker for ulcerative colitis based on intestinal flora and its application, which can overcome the inability of the existing diagnosis of ulcerative colitis to achieve early warning, predict the onset and development. Trends and other shortcomings, can help disease pathology classification and for drug target research, precision drug use, pathogenesis research, etc.

Method used

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  • Ulcerative colitis biomarker based on intestinal flora and application of ulcerative colitis biomarker
  • Ulcerative colitis biomarker based on intestinal flora and application of ulcerative colitis biomarker
  • Ulcerative colitis biomarker based on intestinal flora and application of ulcerative colitis biomarker

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Embodiment 1

[0106] 1.1 Sample collection

[0107] Referring to the method described in the literature (A metagenome-wide association study of gut microbiota intype 2 diabetes.Nature, 2012,490(7418):55-60), the stool samples were collected and transported frozen and quickly transferred to -80°C for storage. Extract to obtain the extracted DNA sample. The stool samples of the subjects with ulcerative colitis and non-ulcerative colitis of the present invention come from China. A total of 99 cases, including 44 healthy samples and 55 ulcerative colitis samples. The study protocol was approved by the Ethics Committee of Qilu Hospital of Shandong University.

[0108] 1.2 Metagenome sequencing and assembly

[0109] The extracted DNA samples were used to construct a sequencing library, and two-way (Paired-end) metagenomic sequencing (insert fragment 350bp, read length 100bp) was performed on the Illumina HiSeq2000 sequencing platform. Filter the data generated by sequencing (quality-controlle...

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Abstract

The invention provides an ulcerative colitis biomarker based on intestinal flora and application of the ulcerative colitis biomarker, and belongs to the technical field of biological medicines. The ulcerative colitis biomarker based on intestinal flora provided by the invention can overcome the defects that the existing ulcerative colitis diagnosis cannot realize early warning, cannot predict themorbidity and development trend and the like; and the ulcerative colitis biomarker based on intestinal flora can help pathological typing of diseases and research on drug action targets, precise medication, pathogenesis and the like, so that a high practical application value is achieved.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a biomarker for ulcerative colitis based on intestinal flora and its application. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Ulcerative colitis (UC), also known as nonspecific ulcerative colitis, is a chronic disease that causes inflammation and ulceration of the colon and rectum. The main symptoms during an attack include abdominal pain and diarrhea with bloody stools. Symptoms of weight loss, fever, and anemia may also occur during an ulcerative colitis attack. Usually, the onset of symptoms is slow, and the severity varies. The symptoms often appear in...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/689
CPCC12Q1/6883C12Q1/689C12Q2600/136
Inventor 左秀丽崔潇李理想李延青
Owner SHANDONG UNIV QILU HOSPITAL
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