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Coronary heart disease patient-specific biomarker composition and use thereof

A biomarker and biomarker technology, applied in the field of biomarker compositions, can solve problems such as trauma and invasiveness

Active Publication Date: 2019-10-25
BGI SHENZHEN CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the invasiveness and invasiveness of existing coronary heart disease diagnostic methods, the problem to be solved by the present invention is to provide a biomarker combination (i.e. a biomarker composition) that can be used for coronary heart disease diagnosis and disease risk assessment, and Methods for diagnosis and risk assessment of coronary heart disease

Method used

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  • Coronary heart disease patient-specific biomarker composition and use thereof
  • Coronary heart disease patient-specific biomarker composition and use thereof
  • Coronary heart disease patient-specific biomarker composition and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] 1.1 Sample collection: The morning urine of the volunteers was collected and immediately stored in a -80°C low-temperature refrigerator. A total of 52 urine samples were collected in the normal group, and a total of 40 urine samples were collected in the coronary heart disease group.

[0086] 1.2 Processing of samples: thaw frozen samples at room temperature, take 500 μL of urine samples into a 2.0 mL centrifuge tube, add 500 μL of methanol to dilute, centrifuge at 10,000 rpm for 5 min, and set aside.

[0087] 1.3 Liquid chromatography-mass spectrometry analysis

[0088] equipment

[0089] HPLC-MS-LTQ Orbitrap Discovery (Thermo, Germany)

[0090] Chromatographic conditions

[0091] Chromatographic column: C18 column (150mm×2.1mm, 5μm); mobile phase A: 0.1% formic acid aqueous solution, mobile phase B: 0.1% formic acid acetonitrile solution; gradient elution program: 0~3min, 5%B, 3~36min, 5%~80%B, 36~40min, 80%~100%B, 40~45min, 100%B, 45~50min, 100%~5%B, 50~60min, 5%...

Embodiment 2

[0099] 2.1 Sample collection: The morning urine of the volunteers was collected and immediately stored in a -80°C low-temperature refrigerator. A total of 52 urine samples were collected in the normal group, and a total of 40 urine samples were collected in the coronary heart disease group.

[0100] 2.2 Processing of samples: thaw frozen samples at room temperature, take 500 μL of urine samples into a 2.0 mL centrifuge tube, add 500 μL of methanol to dilute, centrifuge at 10,000 rpm for 5 min, and set aside.

[0101] 2.3 Liquid chromatography-mass spectrometry analysis

[0102] equipment

[0103] HPLC-MS-LTQ Orbitrap Discovery (Thermo, Germany)

[0104] Chromatographic conditions

[0105] Chromatographic column: C18 column (150mm×2.1mm, 5μm); mobile phase A: 0.1% formic acid aqueous solution, mobile phase B: 0.1% formic acid acetonitrile solution; gradient elution program: 0~3min, 5%B, 3~36min, 5%~80%B, 36~40min, 80%~100%B, 40~45min, 100%B, 45~50min, 100%~5%B, 50~60min, 5%...

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Abstract

A disease-specific metabolite profile is provided, in particular to a biomarker composition screened from the urine-specific metabolite profile of patients with coronary heart disease. As well as the use of the biomarker composition for risk assessment, diagnosis, early diagnosis and pathological staging of coronary heart disease, and methods for risk assessment, diagnosis, early diagnosis and pathological staging of coronary heart disease. The provided biomarker composition can be used for early diagnosis of coronary heart disease, has high sensitivity and good specificity, and has good application prospects.

Description

technical field [0001] The present invention relates to a disease-specific metabolite profile, in particular to a biomarker composition screened from the urine-specific metabolite profile of subjects with coronary heart disease. The present invention also relates to the use of the biomarker composition for risk assessment, diagnosis, early diagnosis and pathological staging of coronary heart disease, and methods for risk assessment, diagnosis, early diagnosis and pathological staging of coronary heart disease. Background technique [0002] Coronary artery heart disease (English: coronary artery heart disease, CAHD), also known as ischemic heart disease, referred to as coronary heart disease, is one of the most common heart diseases, which refers to myocardial infarction caused by coronary artery stenosis and insufficient blood supply. Dysfunction and / or organic disease, also known as ischemic cardiomyopathy (IHD), was the leading cause of death worldwide in 2012 [1] and one ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/62G01N33/48G16B40/20G16H50/20G16H50/30G16H70/40
CPCG01N30/02G01N30/62G01N33/48G01N33/6893G16B40/00G16H50/30G01N33/493G16H70/40G16H50/20G16B40/20G01N30/7233G01N2570/00G01N2800/324G01N2800/50G01N2800/56
Inventor 冯强刘志鹏孟楠王俊
Owner BGI SHENZHEN CO LTD
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