Screening of polycystic ovarian syndrome intestinal flora biomarker and application thereof
A biomarker, polycystic ovary technology, applied in the prognostic monitoring of polycystic ovary syndrome, the field of intestinal flora markers, can solve disease confusion, unfavorable clinical accurate diagnosis and guidance, and heterogeneous clinical manifestations of patients High specificity and sensitivity, comfortable and non-invasive testing, and accuracy guaranteed
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[0044] 1.1 Sample collection
[0045] From March 2018 to May 2019, 23 patients with polycystic ovary syndrome and 23 healthy female controls were selected from the Gynecology Clinic of Guangdong Provincial Hospital of Traditional Chinese Medicine. After collecting stool samples, they were quickly transferred to -80°C for storage and DNA was extracted.
[0046] 1.2 Metagenome sequencing and assembly
[0047] The extracted DNA samples were used to construct a sequencing library, and two-way (Paired-end) metagenomic sequencing (insert fragment 350bp, read length 100bp) was performed on the Illumina HiSeq2000 sequencing platform. Filter the data generated by sequencing (quality-controlled), remove adapter contamination sequences, low-quality sequences and host genome contamination sequences, and obtain high-quality sequencing fragments (reads).
[0048] 1.3 Analysis of marker strains between groups
[0049] Drawing through LEfSe analysis software, and doing linear discriminant a...
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