31results about How to "High specificity and sensitivity" patented technology

A method for detecting variation of gene based on fluorescence quenching quantification comprises: performing single base extension at a specific site of a gene to be detected with marked probes and marked dideoxyribonucleotide triphosphate; detecting fluorescence quenching values, and determining SNP of the gene to be detected. The present invention also provides an oligonucleotide probe for the method.

Disclosed is the development of a protein used as a biomarker for diagnosing IgA nephropathy and TGBM (thin-glomerular-basement-membrane) using urine through a target proteomics method. The disclosed development relates to a diagnosis biomarker protein and a kit for diagnosing IgA nephropathy and TGBM and predicting progress of the nephropathy in advance using the protein. The protein level is increased or decreased in urine from a patient with IgA nephropathy or TGBM nephropathy compared to urine from a normal patient. According to the disclosed development, the degree of the disease can be grasped by detecting IgA nephropathy and TGBM, enabling early diagnosis and confirming progress from the patient's urine. In addition, a monoclonal antibody produced based on the diagnosis biomarker protein can be used for an immunoassay kit (ELISA, antibody coated tube test, lateral-flow test, potable biosensor). As well, the monoclonal antibody is used in early diagnosis and progress detection of IgA nephropathy and development of a novel drug for the purpose of treatment.

The invention discloses a highly sensitive and decomposable quantum dot nanosphere probe and a preparation method thereof. Through a fulcrum-mediated strand displacement reaction, a DNA hairpin probe modified by three desulfated biotins is self-assembled into a Y-type DNA nanostructure; and the Y-type DNA structure and quantum dots coupled to streptavidin are self-assembled to form the quantum dot nanosphere probe. The prepared quantum dot nanosphere probe and an immunomagnetic separation technology are combined to be applied to quick detection of a target analyte. Because of a binding force between biotin and streptavidin stronger thana binding force of desulfurized biotin, the quantum dot nanosphere probe is decomposed, and thus quantum dots are released; the target analyte concentration is determined by detecting the fluorescence value of a quantum dot solution, and a shielding effect of immunomagnetic beads on the quantum dots is overcome. The method has high sensitivity and the detection limit of microorganisms is 1.37 cfu/mL. This method is good in specificity, and common other microorganisms do not affect the detection.

Provided is a method for diagnosing cancer through a difference with a control group in view of the ratio of a deglycosylated peptide fragment and a non-glycosylated peptide fragment in a protein including an N-linked glycosylation motif. Further provided is a method for diagnosing cancer through the detection of the glycosylation ratio in the protein according to the subject matter enables the diagnosis of cancer with high specificity and sensitivity using at least one existing marker, and can be useful in discovering new markers for diagnosing cancer.

Disclosed is a chemiluminescence immunoassay test kit which is used to detect rubella IgG antibody, the test kit includes: positive and negative reference substance, solid-phase carrier which is coated by rubella virus antigen, sample diluent, antihuman IgG monoclonal antibody labeled by alkaline phosphatase, chemiluminescence zymolyte and condensed washing liquid. The preparing method of the test kit includes steps as following: 1) the positive and negative reference substance is prepared; 2) the solid-phase carrier is coated; 3) the sample diluent is prepared; 4) the antihuman IgG monoclonal antibody is labeled by the alkaline phosphatase; 5) the chemiluminescence zymolyte is prepared; 6) the condensed washing liquid is prepared; 7) the positive and negative reference substance, the sample diluent, the alkaline phosphatase labeled compound, the chemiluminescence zymolyte and the condensed washing liquid can be filled and packed through separate packing; 8) finished product can be made after assembly. Adopting the test kit and the preparing method, the rubella IgG antibody in the human blood serum can be detected simply, quickly, sensitively and accurately, thereby being convenient for popularization and application.

The invention discloses an antigenic epitope of a rice grain glutelin GluA2 subunit, an anti-rice grain glutelin GluA2 subunit polyclonal antibody and a preparation method and application thereof. Theantigenic epitope of the rice grain glutelin GluA2 subunit has the amino acid sequence shown as SEQ ID NO.1. antiserum is prepared through polypeptide synthesis, polypeptide and carrier protein coupling and animal immunizing, and a glutelin GluA2 subunit polyclonal antibody is obtained through affinity chromatography and purification. The prepared polyclonal antibody has high titer, strong affinity and good specificity and can produce specific binding reaction with the rice glutelin GluA2 subunit, and an important tool is provided for the basic research of gluten, such as analysis in specificity to its important members, expression profile and content and the study of its related physiological functions.

The invention belongs to the technical field of biological immunology, and more specifically relates to an anti-Ebola virus GP protein monoclonal antibody, and applications thereof. The anti-Ebola virus GP protein monoclonal antibody is secreted by a hybridoma cell strain with a preservation number of CCTCC NO:C2016106. The invention also discloses a preparation method and applications of the anti-Ebola virus GP protein monoclonal antibody. The anti-Ebola virus GP protein monoclonal antibody is high in specificity and biological binding activity, is capable of reacting with sf9 cells infectedby recombinant baculovirus containing GP gene, and is not reacted with normal sf9 cells, so that the anti-Ebola virus GP protein monoclonal antibody can be used for preparation of kits used for detecting Ebola virus GP protein and immunoassay.

The invention discloses DNA methylation markers for diagnosing early liver cancer by using peripheral blood and an application thereof.The invention belongs to the field of biological detection, and relates to the markers for liver cancer and the application thereof, wherein the markers include at least one CpG site selected from the group consisting of CpG sites or modified CpG sites at positions24167,36996,37361,37178,39913,30789,37020,36832,34428, or 34805based on the human ribosomal DNA repeat fragment unit reference sequence U13369.1.The invention further provides a system, a kit and thelike for diagnosing the liver cancer by using the combination of the markers.The methylation state of the markers is obviously different in tumor tissues and non-tumor tissues; the methylation of themarkers is low in the tumor tissues, and the accuracy rate of distinguishing whether or not a patient suffers from liver cancer in a test set by the marker combination reaches 95 percent.

Disclosed herein are diagnostic devices, kits, and methods for the detection of an active Staphylococcus infection in an individual. Utilizing a sample from the individual, antibodies specific for one or more Staphylococcus polypeptides are detected, where the detection of a threshold number of antibodies specific for one or more Staphylococcus polypeptides indicates the presence of an active Staphylococcus infection. Exemplary panels of Staphylococcus polypeptides that can be used with a high degree of specificity and sensitivity are disclosed.

The invention particularly discloses a Dot-ELISA (enzyme-linked immunosorbent essay) quick detection reagent kit for toxoplasmosis serum antibodies of cats. Nitrocellulose (NC) membranes are perforated and then are fixed into 96 orifice plates during experiments, toxoplasmosis SAG3 proteins are enveloped in the NC membranes, to-be-detected antibodies are added into the toxoplasmosis SAG3 proteins and react to the toxoplasmosis SAG3 proteins, and enzyme-labeled secondary antibodies are added into the toxoplasmosis SAG3 proteins to obtain antigen-antibody compositions; color development solution is added into the antigen-antibody compositions, and results are judged after reaction is terminated; the results are positive if dots on the NC membranes are brown and are negative if colors are not changed. The Dot-ELISA quick detection reagent kit has the advantages that the NC membranes are enveloped by the aid of recombinant antigens, and accordingly the storage time can be prolonged; diaphragms can be detached to be independently stored, and accordingly the NC membranes can be prevented from being contaminated; the Dot-ELISA quick detection reagent kit is high in sensitivity and specificity, special instruments can be omitted, serum samples can be screened on a large screen, and accordingly the Dot-ELISA quick detection reagent kit has high practical application value.

The invention provides a method for detecting related substances in a sodium levofolinate raw material and a preparation of the sodium levofolinate raw material, the method is carried out by adoptinga high performance liquid chromatography, and the chromatographic conditions are as follows: a chromatographic column is an octadecylsilane chemically bonded silica chromatographic column; the mobilephase A is a salt solution; the pH value of the salt solution is 6.0 to 9.0; the mobile phase B is a mixed solution of any one or two of acetonitrile, methanol and ethanol; the salt solution is a saltsolution containing an ion pair reagent; a gradient elution method is adopted, the flow velocity of a mobile phase is 0.2-1.2 ml/min, the column temperature of a chromatographic column is 35-45 DEG C, and the detection wavelength is 200-300 nm; the detection method is simple and convenient to operate, high in separation degree, high in specificity and accurate and credible in detection result.

A microscope apparatus for detecting or imaging a target protein using a probe for intrinsic fluorescence resonance energy transfer (iFRET) according to the present invention comprises: a light irradiation unit that irradiates a first light having a wavelength range for exciting an amino acid in the target protein and a second light having a wavelength range for exciting a fluorescent molecule of the probe for iFRET; an objective lens that allows the lights irradiated from the light irradiation unit to be incident onto a sample into which the probe for iFRET is introduced; and a recognition unit that detects a first light emitting signal generated from the probe for iFRET by irradiating the first light having a wavelength range for exciting an amino acid in the target protein onto the sample and a second light emitting signal generated from the probe for iFRET by irradiating the second light having a wavelength range for exciting a fluorescent molecule of the probe for iFRET onto the sample, wherein the probe for iFRET includes: a binding site specific to a target protein or a molecule which has the binding site; and a fluorescent molecule having an acceptor function with respect to intrinsic fluorescence of the target protein, which are bonded to each other directly or by a linker.

The invention discloses a semi-open nuclear magnetic resonance imaging system. The semi-open nuclear magnetic resonance imaging system comprises a magnet housing, an iron yoke fixing seat arranged in the magnet housing, a semi-ring type permanent magnet arranged on the iron yoke fixing seat, a two-dimensional gradient coil arranged on the semi-ring type permanent magnet and close to the open position of the semi-ring type permanent magnet, two hemispherical radio-frequency coils arranged in the semi-ring type permanent magnet and mammary gland supporting plates fixedly connected with the two hemispherical radio-frequency coils. The semi-open nuclear magnetic resonance imaging system has the advantages that compared with a fine standard mammary gland photography technique, the carcinogenic risk caused by ionizing radiation is not produced; compared with an ultrasonic mammary gland screening technique, and nuclear magnetic resonance images are good in soft tissue resolution and have higher specificity and sensitivity; compared with an existing clinic nuclear magnetic resonance mammary gland examination technique, the semi-open nuclear magnetic resonance imaging system is low in device cost, movable, small in limitation to patients, convenient and quick to operate and convenient to position during examination and can be popularized in a large number of township hospitals and can utilize larger constant gradient of the magnet itself to achieve diffusion analysis and diffusion imaging.

The present disclosure provides epigenetic signatures, comprising genomic CpG dinucleotide sequences, genes, and/or genomic regions, which are differentially methylated in individuals with CHARGE syndrome relative to non-CHARGE syndrome controls, and their use in methods and kits for detecting and/or screening for CHARGE syndrome, or the likelihood of CHARGE syndrome. The present disclosure also provides epigenetic signatures, comprising genomic CpG dinucleotide sequences, genes, and/or genomic regions, which are differentially methylated in individuals with Kabuki syndrome relative to non-Kabuki syndrome controls, and their use in methods and kits for detecting and/or screening for Kabuki syndrome, or the likelihood of Kabuki syndrome.

The invention discloses a preparation method of a rice Cu/Zn-SOD polyclonal antibody and application of the rice Cu/Zn-SOD polyclonal antibody, and belongs to the technical field of genetic engineering. By conducting analytic prediction on epitope of rice chloroplast Cu/Zn-SOD protein, an SODCP protein sequence without transporting signal peptide is used for constructing recombinant plasmid; SODCPrecombinant protein is prepared, and is used as an antigen; through antigen immunoreaction, the rice Cu/Zn-SOD polyclonal antibody is prepared. The prepared polyclonal antibody is high in titer, highin affinity and good in specificity, the polyclonal antibody and the rice Cu/Zn-SOD protein can be subjected to specific binding reaction, moreover, the preparation cost is low, the yield is high, and the polyclonal antibody has feasibility of industrial production. By means of the prepared polyclonal antibody, analytical investigation can be conducted on expression situations of rice in different development stages under different growth conditions, and the polyclonal antibody can be applied in all metabolism mechanism studies, which are relative to rice Cu/Zn-SOD protein action, of rice, sothat detection and verification technical support is provided for studies of functions and action mechanisms of the rice Cu/Zn-SOD protein.

The invention provides a kind of detection method of clevidipine butyrate, the method adopts reversed-phase liquid chromatography, and the best chromatographic conditions are as follows: the chromatographic column is a Welchrom C18 chromatographic column; the mobile phase consists of methanol: ethanol: water=15~ 20:40~45:37~42 or acetonitrile:ethanol:water=16:40:44; the flow rate of the mobile phase is 1.0ml/min; the detection wavelength is 240nm; the column temperature is 40°C; the detector is an ultraviolet detector or a diode array detector. The detection method provided by the invention has high sensitivity and specificity, is simple and convenient to operate, and the degree of separation meets the requirements, and can quickly and accurately detect the content of various impurities in clevidipine butyrate, and can be used for the detection of clevidipine butyrate. Quality control is very important for the synthesis of antihypertensive drug clevidipine butyrate.

The invention relates to a polypeptide derivative which specifically binds to atherosclerotic plaques. A detection reagent assesses the degree of risk of cerebral apoplexy caused by falling of the atherosclerotic plaques. The invention further provides a preparation method and application of the detection reagent.

The invention discloses a polycystic ovarian syndrome (PCOS) biomarker combination based on intestinal flora. The polycystic ovarian syndrome (PCOS) biomarker combination is applied to polycystic ovarian syndrome intestinal flora detection, polycystic ovarian syndrome disease risk prediction and polycystic ovarian syndrome prognosis monitoring. The polycystic ovarian syndrome biomarker combination based on intestinal flora is characterized by comprising the following components: 1) a biomarker 1: Ruminococcus_bromii; a biomarker 2: Gordonibacterium_pamelaeae; a biomarker 3: Eesulfovibrio_piger; a biomarker 4: Eubacterium_ventriosum, and a biomarker 5: Prevotella_stercorea; and 2) a polycystic ovarian syndrome biomarker combination diagnosis model based on the intestinal flora has the advantages of high sensitivity, strong specificity and simple result judgment.

The invention relates to a detection method of avian influenza virus H5N1. The detection method is characterized by comprising the following steps of: extracting an RNA (Ribose Nucleic Acid) fragment of target virus; building an amplified reaction system, including RNA and DNA (Desoxvribose Nucleic Acid) polymerized buffer solutions, proper reverse transcriptase, etc.; mixing the substances above with triple extracted medical distilled water; slightly oscillating; introducing a primer; performing warm water bath under a temperature of 35 DEG C; electrically measuring by agarose gel electrophoresis; centrifuging at high speed; suspending; removing supernate; and then comparing with HA (Hyaluronic Acid) gene, wherein the agarose gel electrophoresis is 1-2% in mass percentage; the medical distilled water is warmed for at least 24 hours under room temperature before use so as to keep the temperature in at least 25 DEG C. The detection method has the beneficial effects that the experimental specificity and sensitivity are high; the nucleic acid sequence structure can be described; the operation is relatively simple; and the requirements on conditions are not high.

A detection method and sensors are provided for the rapid detection of chemicals, biological and non-biological, and a wide range of viruses using magnetic tunnel junction-based molecular spintronics devices (MTJMSD) that produce unique magnetic resonance signals before and after interacting with target chemical, biochemical, viral, and other molecular agents.

The invention relates to a multiplex fluorescence PCR kit, a method and application for detecting respiratory pathogens. The kit comprises reagents for detecting adenoviruses, cytomegaloviruses, bocaviruses and mycoplasma pneumoniae, the reagents comprise probes for detecting the above viruses, primer pairs for specifically amplifying corresponding virus target genes, reaction buffer solutions, anenzyme mixture, a positive control and a negative control; and the detection channel of the adenovirus is FAM, the detection channel of the cytomegalovirus is VIC, the detection channel of the bocavirus is Texas Red, and the detection channel of the mycoplasma pneumoniae is Cy5. The kit disclosed by the invention can be used for simultaneously detecting the above four viruses, and has high sensitivity and specificity, at the same time, the operation process is simplified, the detection time is shortened, the detection flux is improved, the detection cost is reduces, and the kit can be universally applied to various fluorescent quantitative PCR instruments.

Disclosed are methods of detecting the likelihood of cancer recurrence. More particularly, the present invention discloses methods of identifying nucleic acid signatures that correlate with the likelihood of cancer recurrence, and methods of using such signatures.

The invention discloses a detection material for resisting CASPR2 autoantibodies in human body fluid, a preparation method and an application. A CASPR2 antigen is arranged on an NC membrane through coating, a detection material for resisting a CASPR2 receptor is prepared, a specific CASPR2 antibody in human serum and cerebrospinal fluid can be combined with the antigen, color development is performed through an alkaline phosphatase substrate-ligand reaction, in a color development reaction, an enclosing material disclosed by the invention is added, and whether CASPR2 antibodies are contained in detection samples can be directly judged through observation by naked eye. The method has the advantages of being high in sensitivity, simple and convenient to operate, quick to detect and the like,and recognition and diagnosis of CASPR2 receptor resistant encephalitis are facilitated.

The invention provides cell factor conjoint analysis as a schizophrenia marker and application of the schizophrenia marker, and particularly provides application of a reagent material and/or instrument equipment for detecting the level of cell factors in a sample from a to-be-detected individual in preparation of a kit for diagnosing schizophrenia. The cell factors comprise G-CSF, IL-2, IL-4, MIG and EGF. When the cell factors are used for conjoint analysis, high sensitivity and high specificity are achieved while the AUC value is high, and a large application prospect is achieved.