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Method for differentiating peripheral blood stem cells into endoderm cells and application thereof

A technology of peripheral blood stem cells and endoderm cells, applied in the direction of blood/immune system cells, animal cells, vertebrate cells, etc., to achieve the effect of wide source of cells, increase the scope of clinical application, and solve the problem of tumorigenesis

Pending Publication Date: 2022-07-29
宫念樵
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the technology of differentiating endoderm cells into islet cells is relatively mature, but there is no mature technology for differentiating peripheral blood stem cells into endoderm cells.

Method used

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  • Method for differentiating peripheral blood stem cells into endoderm cells and application thereof
  • Method for differentiating peripheral blood stem cells into endoderm cells and application thereof
  • Method for differentiating peripheral blood stem cells into endoderm cells and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Specific experimental steps:

[0035] ① Source of peripheral blood stem cells: use a drug mobilizing agent to promote the release of hematopoietic stem cells from the bone marrow into peripheral blood, and then collect hematopoietic stem cells from circulating blood through a blood cell separator. The collection process was carried out completely in a closed environment. The specific acquisition and separation steps are as follows:

[0036] i. Use the drug mobilizing agent recombinant human granulocyte colony-stimulating factor injection (rhG-CSF) to promote the release of hematopoietic stem cells from bone marrow to peripheral blood in healthy volunteers, and collect volunteers' peripheral blood 48 hours later. nourish. The isolation technique of hematopoietic stem cells refers to the existing standard technique.

[0037] ii. Add 6% hydroxyethyl starch (HES) to the obtained peripheral blood at a ratio of 4:1. After the erythrocytes are naturally settled, the upper l...

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PUM

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Abstract

The invention belongs to the technical field of cell differentiation, and particularly relates to a method for differentiating peripheral blood stem cells into endoderm cells and application of the method. According to the invention, peripheral blood stem cells are subjected to induced culture in the presence of BM, Activin A, Wnt3a, TGF-b, LIF, TNF-a, IFN-a, vinterferron-alpha, beta, gamma, B-27 and N2, such that endoderm cells are obtained. The technology is a technical supplement for differentiating the endoderm cells into the islet cells, and the clinical application range of differentiating the stem cells into the islet cells and applying the stem cells to islet transplantation is widened. The technology is expected to solve the islet cell source problem and the islet transplantation heterologous problem, and the clinical effect of islet transplantation and the long-term survival of the graft are improved.

Description

technical field [0001] The invention belongs to the technical field of cell differentiation, and in particular relates to a method for differentiating peripheral blood stem cells into endoderm cells and applications thereof. Background technique [0002] End-stage diabetes is still a major chronic disease that seriously affects the quality of human life so far. my country has become the country with the largest number of diabetes patients in the world. Islet transplantation is currently an effective option for the treatment of end-stage diabetes and an attractive surgical approach. Islet transplantation is currently performed by allogeneic transplantation. Islet transplantation alone can be used to treat diabetes mellitus, and it can also be combined with kidney transplantation or sequential transplantation to treat diabetes complicated by uremia. However, the clinical efficacy of islet transplantation is still unsatisfactory at this stage. One of the main reasons is that ...

Claims

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Application Information

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IPC IPC(8): C12N5/071C12N5/0789
CPCC12N5/0602C12N5/0647C12N5/0676C12N2501/16C12N2501/415C12N2501/15C12N2501/235C12N2501/24C12N2506/11
Inventor 宫念樵赵圆圆
Owner 宫念樵
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