A gel matrix for islet cell transplantation and its preparation method

A technology of gel matrix and islet cells, which is applied in the field of biotechnology and biomedicine, can solve the problems of poor water solubility and low stability of bilirubin, reduce the loss of cell translocation, facilitate storage and use, and maintain the secretory function of islet cells Effect

Active Publication Date: 2021-06-01
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the extremely poor water solubility and low stability of bilirubin, which has significant neurotoxicity, there are few reports on its formulation research in medical applications.
Utilizing the interaction between cyclodextrin and bilirubin to construct a bilirubin drug delivery system has not been reported yet

Method used

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  • A gel matrix for islet cell transplantation and its preparation method
  • A gel matrix for islet cell transplantation and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Preparation of gel matrix for islet cell transplantation

[0023] According to the composition of each experimental group and control group in Table 1, each component was weighed, bilirubin was dissolved in chloroform, β-cyclodextrin grafted ε-polylysine was dissolved in deionized water, and Add bilirubin solution to β-cyclodextrin grafted ε-polylysine aqueous solution, after ultrasonic emulsification, stir and then evaporate to remove organic reagents to obtain bilirubin / β-cyclodextrin grafted ε-polylysine acid solution. Add the bilirubin / β-cyclodextrin grafted ε-polylysine aqueous solution into the fat pad tissue matrix, and stir evenly to obtain the gel matrix for islet cell transplantation. After γ-ray irradiation sterilization, it is sealed and stored, and the islet cell suspension can be added before transplantation.

[0024] Table 1 Composition of the gel matrix used for islet cell transplantation

[0025]

[0026] Note: " / " in missing control gro...

experiment example

[0027] Experimental example In vivo evaluation of the application effect of islet cell transplantation hydrogel in each group

[0028] (1) Establishment of mouse diabetes model

[0029] Use streptozotocin to prepare the mouse type I diabetes model: before the modeling, the mice were fasted without water, and a single intraperitoneal injection of streptozotocin 55mg / kg was performed according to the body weight, and the mice showed polydipsia and polyphagia after 1 week , Polyuria and weight loss, blood sugar value ≥ 13.88mmol / L.

[0030] (2) Preparation of islet cell gel matrix

[0031] 350±50IEQ islets were suspended into the gel matrix for islet cell transplantation of each experimental group in Example 1.

[0032] (3) Transplantation of pancreatic islet cells under the kidney capsule of the mouse diabetic model

[0033] Diabetic mice were subcutaneously injected with 50 μL of cefazolin sodium (concentration: 50 mg / mL) 15 minutes before operation, and intraperitoneally in...

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Abstract

The invention provides a gel matrix for pancreatic islet cell transplantation and a preparation method thereof. The gel matrix is ​​composed of fat pad tissue matrix, β-cyclodextrin grafted with ε-polylysine, bilirubin and water. The gel matrix can be used for islet cell transplantation, and the success rate of the transplantation is improved. The present invention provides a new matrix for islet cell transplantation, which provides physical support after transplantation by optimizing the ratio, reduces the cell loss of islet cells caused by immune and inflammatory reactions, promotes vascularization at the transplantation site, and maintains islet cells in vivo activity and function. The gel matrix overcomes the technical bottleneck that the existing matrices cannot meet the requirements of transplantation, provides a new type of gel matrix that can maintain the viability and mechanical support of islet cells, and provides the best physical, chemical and biological microbiological conditions for islet cells to function after transplantation. environment and has broad application prospects.

Description

technical field [0001] The invention relates to the fields of biotechnology and biomedicine, in particular to a gel matrix for islet cell transplantation and a preparation method thereof. Background technique [0002] Type 1 diabetes is a chronic metabolic disease characterized by reduced or complete loss of insulin production. The disease mostly occurs in adolescents, and patients are prone to ketoacidosis, which can even be life-threatening. People with type 1 diabetes need long-term insulin injections to control blood sugar. However, even if the patient uses insulin, his blood sugar fluctuates greatly. At present, pancreas transplantation is an effective way to treat the disease, but the cost of pancreas transplantation is high, the success rate is low, and the source of pancreas is in short supply. Islet cell transplantation can rebuild pancreas function in patients, and patients can reduce insulin use or stop insulin completely after transplantation. However, islet ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/36A61L27/38A61L27/52A61L27/54
CPCA61L27/20A61L27/3633A61L27/3804A61L27/52A61L27/54A61L2300/216A61L2300/30A61L2300/41A61L2300/412C08L87/005
Inventor 姚情赵应征鲁翠涛寇龙发徐荷林黄志伟江雪岳蒙
Owner WENZHOU MEDICAL UNIV
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