Gel substrate for transplanting of pancreatic cells and preparing method of gel substrate

A technology of gel matrix and islet cells, which is applied in the fields of biotechnology and biomedicine, can solve the problems of low stability and poor water solubility of bilirubin, and achieve easy storage and use, reduce cell translocation loss, and good biocompatibility Effect

Active Publication Date: 2019-09-10
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the extremely poor water solubility and low stability of bilirubin, which has significant neurotoxicity, there are few reports on its formulation research in medical applications.
Utilizing the interaction between cyclodextrin and bilirubin to construct a bilirubin drug delivery system has not been reported yet

Method used

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  • Gel substrate for transplanting of pancreatic cells and preparing method of gel substrate
  • Gel substrate for transplanting of pancreatic cells and preparing method of gel substrate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Preparation of gel matrix for islet cell transplantation

[0023] According to the composition of each experimental group and control group in Table 1, each component was weighed, bilirubin was dissolved in chloroform, β-cyclodextrin grafted ε-polylysine was dissolved in deionized water, and Add bilirubin solution to β-cyclodextrin grafted ε-polylysine aqueous solution, after ultrasonic emulsification, stir and then evaporate to remove organic reagents to obtain bilirubin / β-cyclodextrin grafted ε-polylysine acid solution. Add the bilirubin / β-cyclodextrin grafted ε-polylysine aqueous solution into the fat pad tissue matrix, and stir evenly to obtain the gel matrix for islet cell transplantation. After γ-ray irradiation sterilization, it is sealed and stored, and the islet cell suspension can be added before transplantation.

[0024] Table 1 Composition of the gel matrix used for islet cell transplantation

[0025]

[0026] Note: " / " in missing control gro...

experiment example

[0027] Experimental example In vivo evaluation of the application effect of islet cell transplantation hydrogel in each group

[0028] (1) Establishment of mouse diabetes model

[0029] Use streptozotocin to prepare the mouse type I diabetes model: before the modeling, the mice were fasted without water, and a single intraperitoneal injection of streptozotocin 55mg / kg was performed according to the body weight, and the mice showed polydipsia and polyphagia after 1 week , Polyuria and weight loss, blood sugar value ≥ 13.88mmol / L.

[0030] (2) Preparation of islet cell gel matrix

[0031] 350±50IEQ islets were suspended into the gel matrix for islet cell transplantation of each experimental group in Example 1.

[0032] (3) Transplantation of pancreatic islet cells under the kidney capsule of the mouse diabetic model

[0033] Diabetic mice were subcutaneously injected with 50 μL of cefazolin sodium (concentration: 50 mg / mL) 15 minutes before operation, and intraperitoneally in...

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Abstract

The invention provides a gel substrate for transplanting of pancreatic cells and a preparing method of the gel substrate. The gel substrate is composed of a fat pad tissue substrate, beta-cyclodextringrafted epsilon-polylysine, bilirubin and water. The gel substrate can be used for transplanting of the pancreatic cells, and the transplanting success rate of the pancreatic cells is increased. Thenew substrate is provided for transplanting of the pancreatic cells, by optimizing the ratio, physical supporting after transplanting is provided, cell loss caused by immunity and inflammatory reactions on the pancreatic cells is reduced, vascularization of transplanted parts is promoted, and the activity and function of the pancreatic cells in the body are maintained. The gel substrate overcomesthe technical difficulty that existing substrates cannot meet the transplanting requirements; the novel gel substrate for maintaining the viability and mechanics supporting effect of the pancreatic cells is provided, the best physicochemical and biological microenvironment is provided for the pancreatic cells to play a role after transplanting, and the gel substrate has a wide application prospect.

Description

technical field [0001] The invention relates to the fields of biotechnology and biomedicine, in particular to a gel matrix for islet cell transplantation and a preparation method thereof. Background technique [0002] Type 1 diabetes is a chronic metabolic disease characterized by reduced or complete loss of insulin production. The disease mostly occurs in adolescents, and patients are prone to ketoacidosis, which can even be life-threatening. People with type 1 diabetes need long-term insulin injections to control blood sugar. However, even if the patient uses insulin, his blood sugar fluctuates greatly. At present, pancreas transplantation is an effective way to treat the disease, but the cost of pancreas transplantation is high, the success rate is low, and the source of pancreas is in short supply. Islet cell transplantation can rebuild pancreas function in patients, and patients can reduce insulin use or stop insulin completely after transplantation. However, islet ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/36A61L27/38A61L27/52A61L27/54
CPCA61L27/20A61L27/3633A61L27/3804A61L27/52A61L27/54A61L2300/216A61L2300/30A61L2300/41A61L2300/412C08L87/005
Inventor 姚情赵应征鲁翠涛寇龙发徐荷林黄志伟江雪岳蒙
Owner WENZHOU MEDICAL UNIV
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