3D bioprinting forming method

Abstract

The invention discloses a 3D bioprinting forming method. The 3D bioprinting forming method comprises the following steps: (1), performing 3D bioprinting: printing 3D printing bio-ink according to an established model by using a 3D bioprinter, and storing a printing bracket at low temperature to obtain a shaped gel material; (2), crosslinking: washing the shaped gel material by using water; then adding a crosslinking agent, and performing a crosslinking reaction under a low-temperature condition; finally, washing away the residual crosslinking agent by using water. By the 3D bioprinting formingmethod, low-temperature treatment and a crosslinking method are adopted for printing a target structure, so that rapid progress of 3D printing can be guaranteed, covalent bonds are further introducedinside collagen molecules and between the molecules, most of a collagen structure is retained, and the mechanical strength and the chemical stability of the printing material are improved, the absorption speed thereof in a body are controlled, and thus a collagen biomaterial plays a role conveniently.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a 3D bioprinting molding method. Background technique [0002] The cases of 3D printing technology used in clinical medicine are no longer uncommon. The combination of this technology and medical treatment mainly includes the following types: ①preoperative diagnosis and surgical rehearsal model; ②implant preparation (bones, teeth, soft tissue organs, etc.) ; ③ External medical consumables (maxillofacial shaping braces, etc.). At present, the printing technology of hard tissue scaffolds such as bones and teeth is relatively mature, but the printing of soft tissue scaffolds still needs to be further improved. [0003] Most of the existing bio-inks available for soft tissue printing are polymer hydrogels, including artificially synthesized polymer materials and natural hydrogels such as collagen. The effect is weak; cells seeded on the printed scaffold only grow in situ throughout the...

AI Technical Summary

Problems solved by technology

Therefore, the final results of these methods are often unsatisfactory

[0005] Finally, limited by the physical properties of traditional soft tissue 3D printing inks (it is difficult to quickly process them into pre-designed shapes and structures), the existing soft tissue 3D printing technologies generally use cross-linking while printing, or first cross-linking and then The main disadvantages of the printing method include: ① need to use photo-crosslinkable materials, which limits the available materials, has biological safety hazards, and due to beam scattering, more serious problems will occur when preparing materials with smaller sizes. deformation; ②The crosslinking reaction takes a certain amount of time, making the structure of the material unstable during the printing process, and the overall preparation time is too long

[0006] In summary, the biological 3D soft tissue printing technology in the prior art is still immature and cannot meet the needs of scientific research and clinical practice.

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