Dynamic perfusion culture system for cell microsphere

Abstract

The invention provides a dynamic perfusion culture system for a cell microsphere. The system comprises a culture bin, a cell microsphere printing device and a perfusion culture device, the cell microsphere printing device comprises a printing head, the cell microsphere can be printed by the printing head, and the culture bin is used for accommodating and culturing the cell microsphere; the perfusion culture device comprises a liquid storage pot and a first power source, the liquid storage pot is used for storing culture liquid and being communicated with the culture bin, the first power sourceis arranged at a pipe communicating the liquid storage pot with the culture bin, and the culture liquid circularly flows between the liquid storage pot and the culture bin under driving of the firstpower source. The dynamic perfusion culture system for the cell microsphere is high in automation degree, in combination with the cell microsphere printing device and the perfusion culture device, a 3D bioprinting technology and a perfusion culture technology are utilized for culturing cells, and a feasible scheme is provided for characteristic keeping and efficient large-scale multiplication of the cells. The full-automatic multiplication culture system which is urgently needed by modern medical treatment, keeps characteristics of the cells and can efficiently obtain the high-quality cells isprovided.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and in particular to a dynamic perfusion culture system of cell microspheres. Background technique [0002] In the prior art, cell acquisition and cell dynamic culture are carried out in stages, and the cells are placed in an in vitro culture bottle or an incubator for culture, and the culture bottle is manually shaken at predetermined intervals to achieve simple dynamic culture. [0003] This culture method is complicated to operate, time-consuming and labor-intensive, and it is easy to cause foreign objects to float into the culture container, increasing the risk of contamination, and if the fresh culture medium cannot be replaced in time, it will also cause slow cell growth and affect the efficiency of cell expansion. [0004] In addition, the cells are limited by the surface area of ​​the culture container. When the cells proliferate to a certain extent, they must be subcultured and diges...

AI Technical Summary

Problems solved by technology

[0006] This culture system has the disadvantages of cumbersome cell acquisition steps, complicated operation, low efficiency and high cost of perfusion culture

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