Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing cartilage with high mechanical properties based on 3D bioprinting

A technology of bioprinting and cartilage, applied in the field of 3D bioprinting, can solve the problems of low cell survival rate and poor mechanical properties, and achieve the effect of improving mechanical properties, excellent mechanical properties, and not easy to break

Active Publication Date: 2016-09-07
西安博恩生物科技有限公司
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for preparing cartilage with high mechanical properties based on 3D bioprinting to solve the problems of poor mechanical properties and low cell survival rate in bionic cartilage in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing cartilage with high mechanical properties based on 3D bioprinting
  • Method for preparing cartilage with high mechanical properties based on 3D bioprinting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Silk fiber preparation: cut silkworm cocoons into pieces with a side length of 0.5 cm, put the pieces into a sodium carbonate solution with a volume molar concentration of 0.02M, stir and boil for 40 minutes, rinse the silk three times for 20 minutes, take it out with tweezers and dry it overnight. Drop a lithium bromide solution with a volume molar concentration of 10.0M on the silk and incubate at 70°C for 3 hours, put the cultured silk into a dialysis box and dialyze in ultrapure water for 24 hours, take out the silk solution in the dialysis box, centrifuge twice, and incubate at 4°C Save it.

[0023] (2) Preparation of gelatin solution: Add 7.0% wt gelatin in phosphate buffer solution to the phosphate buffer solution, stir continuously at 45°C until the gelatin is completely dissolved, and add 1.5 mL to the solution while stirring at a speed of 0.5 mL / min. A methacrylic anhydride solution with a mass concentration of 94% was reacted at 50°C for 1 hour, then dilu...

Embodiment 2

[0029] (1) Silk fiber preparation: cut the silkworm cocoons into pieces with a side length of 1.0 cm, put the pieces into a sodium carbonate solution with a volume molar concentration of 0.02M, stir and boil for 50 minutes, rinse the silk three times for 20 minutes, take it out with tweezers and dry it overnight. Drop a lithium bromide solution with a volume molar concentration of 7.0M on the silk and incubate at 50°C for 5 hours, put the cultured silk into a dialysis box and dialyze in ultrapure water for 48 hours, take out the silk solution in the dialysis box, centrifuge twice, and incubate at 4°C Save it.

[0030](2) Preparation of gelatin solution: Add 10.0% wt gelatin in phosphate buffer to phosphate buffer, stir continuously at 60°C until the gelatin is completely dissolved, and add 0.5 mL to the solution while stirring at a speed of 1.0 mL / min. A methacrylic anhydride solution with a mass concentration of 94% was reacted at 50°C for 1 hour, then diluted with 40 mL of p...

Embodiment 3

[0036] (1) Silk fiber preparation: cut the silkworm cocoon into 0.8cm pieces with a side length, put the pieces into a sodium carbonate solution with a volume molar concentration of 0.02M, stir and boil for 30min, rinse the silk three times for 20min, take it out with tweezers and dry it overnight. Drop a lithium bromide solution with a volume molar concentration of 12.0M on the silk and incubate at 30°C for 2 hours, put the cultured silk into a dialysis box and dialyze in ultrapure water for 60 hours, take out the silk solution in the dialysis box, centrifuge twice, and incubate at 4°C Save it.

[0037] (2) Preparation of gelatin solution: Add 12% wt of gelatin in phosphate buffer to phosphate buffer, stir continuously at 30°C until the gelatin is completely dissolved, add 1mL of mass to the solution while stirring at a speed of 0.1mL / min The methacrylic anhydride solution with a concentration of 94% was reacted at 50°C for 1h, then diluted with 40mL phosphate buffer at 40°C,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for preparing cartilage with a high mechanical property based on 3D bioprinting. The method comprises the following steps: preparing silk fibers, preparing a gelatin solution, preparing ciological ink containing cells, carrying out 3D cartilage printing, performing cell culture and differentiation on a printed tissue to form the cartilage. The method solves the problems of polarization of 2-dimension culture for cells and high body and surface ratio of cells through the 3D bioprinting technology; sodium alginate and calcium ions form ionic bonds, and gelatin and methacrylic anhydride form covalent bonds, so that the 3D bioprinted cartilage is good in mechanical property and is not easily broken; the gelatin has biocompatibility and degradability of normal biological material hydrogel, has bioactivity, and is good for cell growth and differentiation and realization of cell functions.

Description

technical field [0001] The invention belongs to the technical field of 3D bioprinting, and particularly relates to a method for preparing cartilage with high mechanical properties and an environment favorable for cell growth and differentiation based on 3D bioprinting. Background technique [0002] At present, most of the scaffold materials used in bionic cartilage research are gel materials. PVA and PEG have been extensively studied due to their non-toxic, hydrophilic, degradable and cytocompatibility, but these hydrogels have small tensile fracture energy. About 10Jm ‐ 2 (Cartilage is about 1000Jm ‐ 2 ), the cracks in the gel have a great influence on its mechanical properties, and the mechanical properties are poor; the synthetic hydrogels on the market have excellent mechanical properties, the tensile fracture energy is large, and the cracks in the gel have little influence on their mechanical properties, but these hydrogels Glue cannot be used for cell culture; none...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/22A61L27/52
Inventor 袁方臧剑锋戴祖明裴梦婷黄琦
Owner 西安博恩生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com