Nanocellulose-containing bioinks for 3D bioprinting, methods of making and using the same, and 3D biostructures obtained therefrom

A technology of nanocellulose and bio-ink, which can be used in drug delivery, tissue regeneration, and pharmaceutical formulations, and can solve the problems of non-existence of bio-ink

Pending Publication Date: 2021-03-19
GRANBIO INTELLECTUAL PROPERTY HOLDINGS LLC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] Despite widespread interest in 3D bioprinting research, no truly universal bioink exists to date

Method used

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  • Nanocellulose-containing bioinks for 3D bioprinting, methods of making and using the same, and 3D biostructures obtained therefrom
  • Nanocellulose-containing bioinks for 3D bioprinting, methods of making and using the same, and 3D biostructures obtained therefrom
  • Nanocellulose-containing bioinks for 3D bioprinting, methods of making and using the same, and 3D biostructures obtained therefrom

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example

[0322] The bioprintability of crystalline, fibrillated, and blended formulations of nanocellulose was evaluated by evaluating resolution (gridline analysis) and post-printing shape fidelity and rheology (elasticity, viscosity, and shear thinning properties) and comparing these to pure alginate bioinks. Human nasal septal chondrocytes bioprinted an optimized nanocellulose-alginate bioink to determine cytotoxicity, metabolic activity, and biostructural morphology.

[0323] As will be shown in these examples, the disclosed nanocellulose-alginate bioink composition exhibits a high degree of shear thinning with reversible stress-softening behavior, which facilitates post-printing shape fidelity . Bioinks containing a blend of crystalline and fibril nanocellulose exhibited nanoscale as well as microscale roughness for cell survival and differentiation, and maintained the most stable construct volumes in culture. Human nasal septal chondrocytes exhibit high metabolic activity after...

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Abstract

Some variations of the invention provide a bioink composition for 3D bioprinting, comprising: nanocellulose in the form of nanocellulose crystals, nanocellulose fibrils, or preferably a combination thereof; alginate that is ionically crosslinkable in the presence of an ionic crosslinking agent; and water. The nanocellulose-alginate bioinks have favorable rheological, swelling, and biocompatibilityproperties for extrusion-based bioprinting. It is experimentally demonstrated that nanocellulose-alginate bioinks with human nasoseptal chondrocytes enable cartilage bioprinting at high resolution. The disclosed nanocellulose has been proven to be compatible with cell survival and proliferation; to possess nanoscale and microscale architectures mimicking the native extracellular environment, encouraging differentiation and tissue formation; and to have ideal rheological properties to allow extrusion 3D bioprinting. Bioink with a unique blend of crystal and fibril nanocellulose produces a verystable construct volume in culture.

Description

[0001] priority data [0002] This international patent application claims priority to U.S. Provisional Patent Application No. 62 / 639,538, filed March 7, 2018, which is hereby incorporated by reference herein. technical field [0003] The present invention relates generally to 3D bioprinting, bioink compositions for 3D bioprinting, methods of making and using bioink compositions, and 3D biostructures produced by 3D bioprinting. Background technique [0004] Three-dimensional (3D) printing, or additive manufacturing, is a process by which an object is created in its three-dimensional form using a specialized printer. The printer receives instructions from a design file created in the computer with the help of a 3D modeling program. The file or data blueprint of the object to be printed is then sliced ​​into two-dimensional (2D) representations that are sent to the printer. Multiple layers of material are built based on the information contained in the file; these layers are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/26A61L27/38A61L27/48A61L27/56B33Y80/00B33Y70/00
CPCB33Y70/00B33Y80/00A61L27/26A61L27/3817A61L27/48A61L27/56A61L2400/12A61L2430/06C08L5/04A61L27/3683A61L27/54
Inventor 金伯利·纳尔逊伊恩·惠特克齐塔·杰索普艾莎·阿尔萨巴
Owner GRANBIO INTELLECTUAL PROPERTY HOLDINGS LLC
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