Tissue engineering bone complex and application

A tissue engineering, complex technology, applied in prosthesis, medical science, etc.

Inactive Publication Date: 2007-12-12
SHANDONG UNIV
View PDF0 Cites 21 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, how to construct an ideal bone tissue engineering composite material and its preparation and application, that is, how to prepare a good bone substitute material, make it co-cultured with seed cells, and how to add signal factors and other specific methods have not yet been studied. See the report

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tissue engineering bone complex and application
  • Tissue engineering bone complex and application
  • Tissue engineering bone complex and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Embodiment 1 artificial bone scaffold preparation

[0067] Material preparation:

[0068] The preparation of nano-hydroxyapatite (nano HA powder) was prepared by conventional chemical precipitation; N, O-carboxymethyl chitosan was purchased from Qingdao Haihui Biological Engineering Co., Ltd., with a carboxymethyl substitution degree of 90.5%, The degree of deacetylation is 88.6%; p-dichlorobenzene, absolute ethanol, and citric acid are all analytically pure.

[0069] (1) Nano-hydroxyapatite and carboxymethyl chitosan are proportioned respectively in the ratio of 6: 4 by mass ratio, and are fully mixed with mechanical ball milling;

[0070] (2) Then weigh the nano-hydroxyapatite / carboxymethyl chitosan composite powder and the pore-forming agent p-dichlorobenzene in a mass ratio of 1:1, grind and mix, and then ultrasonically vibrate for 2 hours to make it Mix well;

[0071] (3) adding mass percent in the mixture described in step (2) is 16.7% citric acid solution and ...

Embodiment 2

[0075] Embodiment 2 artificial bone scaffold preparation

[0076] (1) Nano-hydroxyapatite and carboxymethyl chitosan are proportioned respectively in a ratio of 7: 3 by mass ratio, and are fully mixed with mechanical ball milling;

[0077] (2) Then, the nano-hydroxyapatite / carboxymethyl chitosan composite powder and the pore-forming agent p-dichlorobenzene are weighed in a ratio of 1: 1.2 by mass, ground and mixed, and then ultrasonically oscillated for 2.5 hours to make It is thoroughly mixed;

[0078] (3) adding mass percent in the mixture described in step (2) is 16.7% citric acid solution and reconcile evenly, the citric acid solution consumption is as the criterion with the plasticity of mixture;

[0079] (4) Put the forming mixture into the artificial bone support standard mold, press forming, and keep the pressure for 80s;

[0080] (5) Demoulding, first place the formed composite material in the air for 4 hours, then place it in absolute ethanol, ultrasonically vibrat...

Embodiment 3

[0082] Embodiment 3 artificial bone scaffold preparation

[0083] (1) Nano-hydroxyapatite and carboxymethyl chitosan are proportioned respectively in the ratio of 6.5: 3.5 by mass ratio, and are fully mixed with mechanical ball milling;

[0084] (2) Then weigh the nano-hydroxyapatite / carboxymethyl chitosan composite powder and the pore-forming agent p-dichlorobenzene in a mass ratio of 1:1.1, grind and mix, and then ultrasonically vibrate for 3 hours to make it Mix well;

[0085] (3) adding mass percent in the mixture described in step (2) is 16.7% citric acid solution and reconcile evenly, the citric acid solution consumption is as the criterion with the plasticity of mixture;

[0086] (4) Put the forming mixture into the artificial bone support standard mold, press forming, and hold the pressure for 100s;

[0087] (5) Demoulding, first place the formed composite material in air for 5 hours, then place it in absolute ethanol, ultrasonically vibrate for 10 hours, and then dr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
sizeaaaaaaaaaa
sizeaaaaaaaaaa
compressive strengthaaaaaaaaaa
Login to view more

Abstract

The invention discloses a tissue engineered bone compound which comprises porous perforated between aperture artificial bone bracket and mesenchymal stem cells implanted in hole and transfected with vascular endothelial growth factor gene. The invention also discloses the application of the tissue engineered bone compound as material for bone defect repair. The inventive tissue engineered bone compound can make seed cell exert osteogenesis, express and secrete VEGF, thereby promoting vasal formation and growth, providing material basis for new bone formation, in favor of neogenesis of vascularized tissue engineered bone tissue, improving effect for repairing bone defect.

Description

technical field [0001] The invention belongs to the technical field of constructing artificial bone by tissue engineering method in biomedical engineering, and in particular relates to a tissue-engineered bone complex and its construction method and application. Background technique [0002] Bone defects caused by trauma, infection, tumor and congenital diseases in life activities are very common, especially the incidence of bone defects caused by limb trauma is as high as 10%. The treatment and repair of bone defects is a common clinical difficulty in orthopedics one. Previous studies have shown that the diameter of the diaphysis of the long tubular bone determines the length of the bone defect that can heal itself. When the length of the diaphysis bone defect exceeds 1.5 to 2.5 times its diameter, it is difficult to heal itself, and bone graft materials are needed for repair. Due to the advantages and disadvantages of various bone repair materials, they cannot meet the re...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/38
Inventor 赵冬梅王海斌刘军莉赵萍赫淑倩霍延青司海朋
Owner SHANDONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap