The invention discloses a tissue-engineered bone. The tissue-engineered bone is multilayer
cell sheet lamination compound with a three-dimensional
capillary network, the multilayer
cell sheet lamination compound consists of n laminated
bone marrow mesenchymal stem cell sheets and vascular endothelial cells among all
bone marrow mesenchymal stem cell sheet
layers, and n is in a range of 3-8. A preparation method of the tissue-engineered bone comprises steps as follows: firstly, a photosensitive
semiconductor structural layer is prepared on the surface of a
cell culture dish, so that a
photosensitive cell culture dish is obtained; then
bone marrow mesenchymal stem cells are cultured with the
photosensitive cell culture dish, and the bone marrow
mesenchymal stem cell sheets are obtained; finally, the multilayer
cell sheet lamination compound consisting of multiple
layers of the bone marrow mesenchymal
stem cell sheets and the vascular endothelial cells is constructed and cultured to obtain the tissue-engineered bone. The tissue-engineered bone purely consists of homologous cells, so that
pollution caused by immunological rejection and
stent degradation is reduced, and the tissue-engineered bone is significant in
bone defect repair and early vascularization; the method is simple, easy to implement and convenient to popularize.