The invention discloses a tissue-engineered bone. The tissue-engineered bone is multilayer 
cell sheet lamination compound with a three-dimensional 
capillary network, the multilayer 
cell sheet lamination compound consists of n laminated 
bone marrow mesenchymal stem cell sheets and vascular endothelial cells among all 
bone marrow mesenchymal stem cell sheet 
layers, and n is in a range of 3-8. A preparation method of the tissue-engineered bone comprises steps as follows: firstly, a photosensitive 
semiconductor structural layer is prepared on the surface of a 
cell culture dish, so that a 
photosensitive cell culture dish is obtained; then 
bone marrow mesenchymal stem cells are cultured with the 
photosensitive cell culture dish, and the bone marrow 
mesenchymal stem cell sheets are obtained; finally, the multilayer 
cell sheet lamination compound consisting of multiple 
layers of the bone marrow mesenchymal 
stem cell sheets and the vascular endothelial cells is constructed and cultured to obtain the tissue-engineered bone. The tissue-engineered bone purely consists of homologous cells, so that 
pollution caused by immunological rejection and 
stent degradation is reduced, and the tissue-engineered bone is significant in 
bone defect repair and early vascularization; the method is simple, easy to implement and convenient to popularize.