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Preparation method of umbilical cord Huatong glue mesenchymal stem cells and product thereof

A technology of mesenchymal stem cells and Huatong glue, which is applied in the field of preparation of primitive mesenchymal stem cells, can solve the problems of restricting wide application, inability to apply, and reducing the potential of differentiation and homing

Active Publication Date: 2010-01-20
PLA NAVY GENERAL HOSIPTAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, various problems have been found in the research and application of seed cells. For example, the application of embryonic stem cells has ethical, legal, immune rejection and tumorigenicity issues; autologous bone marrow mononuclear cells have complex components and multi-directional differentiation potential. The content of stem cells is low, and the differentiation potential is limited; the source of autologous bone marrow MSCs is limited, the cell proliferation and differentiation potential decreases significantly with age, and long-term expansion in vitro reduces the differentiation and homing potential in vivo
[0003] Compared with autologous MSCs, umbilical cord blood, umbilical cord, and placental MSCs have sufficient sources, low probability of virus contamination, weak immunogenicity, more primitive cells, and no social, ethical, and legal disputes, but the isolation efficiency of umbilical cord blood-derived MSCs is low. Limits its wide application in cell therapy and tissue engineering; the purity of cells isolated from umbilical cord and placenta is low, often mixed with vascular endothelial cells and cannot be used clinically
[0004] The above shows that there is currently no ideal seed cell to meet clinical needs, and it is necessary to expand the source of pluripotent stem cells

Method used

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  • Preparation method of umbilical cord Huatong glue mesenchymal stem cells and product thereof
  • Preparation method of umbilical cord Huatong glue mesenchymal stem cells and product thereof
  • Preparation method of umbilical cord Huatong glue mesenchymal stem cells and product thereof

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Embodiment 1～6

[0027] The following 6 examples are listed, and the implementation methods of the present invention are not limited to these 6 examples. The selection of some optional experimental materials, the practical application of some adjustable process technical parameters, and the test indicators of the finished products obtained in each embodiment are listed as follows.

[0028]

[0029] Based on the contents listed in the above table, according to the method of the present invention, the process steps of using human umbilical cord Huatong jelly to prepare original mesenchymal stem cells are as follows:

[0030] (1) Selection of materials: select healthy pregnant women who are negative for 4 immune items including hepatitis B surface antigen, hepatitis C antibody, syphilis antibody, and AIDS antibody and who are about to undergo caesarean section. The umbilical cord is cut during the cesarean section, quickly placed in D-Hank balance solution and sent to the cell culture room, an...

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Abstract

The invention discloses a preparation method of original mesenchymal stem cells. In the invention, the mesenchymal stem cells are prepared by adopting fresh human umbilical cord, extracting Huatong glue, digesting by collagenase, and separating and cultivating, and the mesenchymal stem cells are suitable for cryopreservation. The invention also relates to the original mesenchymal stem cells prepared by the method, and the original mesenchymal stem cells are verified as mesenchymal stem cells by the identification of biological phenotype according to standard formulated by the International Society of Cytotherapy. The multiplication capability and the purity of the mesenchymal stem cell are both higher than those of bone mesenchymal stem cells; and the amount of the released growth factorsand cell factors is higher than that of the bone mesenchymal stem cells.

Description

technical field [0001] The present invention relates to a method for preparing primitive mesenchymal stem cells, in particular to a method for preparing mesenchymal stem cells from human umbilical cord Wharton'jelly and its products. Background technique [0002] Contemporary cell biology studies have shown that mesenchymal stem cells (MSCs) with self-renewal and multilineage differentiation potential can be applied to the treatment and rehabilitation of various diseases. However, various problems have been found in the research and application of seed cells. For example, the application of embryonic stem cells has ethical, legal, immune rejection and tumorigenicity issues; autologous bone marrow mononuclear cells have complex components and multi-directional differentiation potential. The content of stem cells is low, and the differentiation potential is limited; the source of autologous bone marrow MSCs is limited, the cell proliferation and differentiation potential decli...

Claims

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Application Information

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IPC IPC(8): C12N5/08
Inventor 高连如张宁坤杨晔丁青艾朱智明陈宇曹毅
Owner PLA NAVY GENERAL HOSIPTAL
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