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Method for obtaining neural progenitor cells induced by bone marrow mesenchymal stem cells

A technology of neural precursor cells and bone marrow mesenchyme, applied in the direction of bone/connective tissue cells, animal cells, nervous system cells, etc., can solve the problems of poor system repeatability, poor activity, unable to obtain functional cells, etc. effect of the problem

Inactive Publication Date: 2008-07-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although it has been reported that MSCs have the potential to differentiate into nerve cells, the induction system still needs to be improved, the system repeatability is poor or the activity of the cells after induction is poor, and finally the functional cells cannot be obtained.

Method used

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  • Method for obtaining neural progenitor cells induced by bone marrow mesenchymal stem cells
  • Method for obtaining neural progenitor cells induced by bone marrow mesenchymal stem cells
  • Method for obtaining neural progenitor cells induced by bone marrow mesenchymal stem cells

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Embodiment 1

[0029] 1. Materials and methods

[0030] 1.1 Experimental materials

[0031] 1.1.1 Experimental animals: SD rats, 4-5 weeks old, weighing 80-120 g, were purchased from the Experimental Animal Center of Zhejiang University.

[0032] 1.1.2 Experimental reagents

[0033] 1.1.2.1 General reagents

[0034] 25cm 2 Culture flasks Orange scientific Belgium

[0035] 6-well culture plate Becton Dickinson, USA

[0036] PBS solution Gino Biomedical Technology Co., Ltd. China

[0037] 0.25% Trypsin GIBCO Corporation USA

[0038] Polylysine solution SIGMA Corporation USA

[0039] Premium Fetal Bovine Serum FBS Hangzhou Sijiqing Biological Engineering Co., Ltd. China

[0040] Low sugar DMEM solution GIBCO company USA

[0041] N2 adjuvant GIBCO / invitrogen USA

[0042] 2.1.2.2 Cell induction reagents

[0043] EGF CYTOLAB / PEPROTECH USA

[0044] bFGF CYTOLAB / PEPROTECH USA

[0045] IGF-1 CYTOLAB / PEPROTECH USA

[0046] BDNF CYTOLAB / PEPROTECH USA

[0047] NT-3 CYTOLAB / PEPROTECH USA ...

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Abstract

The invention provides a method for inducing mesenchymal stem cell in the bone marrow to obtain the nerve prosoma cell, which comprises the following steps: doing generation culture for the separated and purified mesenchymal stem cell in the bone marrow, switching stable generation mesenchymal stem cell into induction culture medium, inducing and culturing for 7-20 d, obtaining nerve prosoma cell. The induction culture medium is basic culture medium which is charged with growth factor, wherein the growth factor mainly comprises an epiderm growth factor (EGF), a base fibroblast growth factor (bFGF), an insulin growth factor-1 (IGF-1) and a nerve nourishment factor 3 (NT-3). The invention provides a method for inducing mesenchymal stem cell in the bone marrow to obtain the nerve prosoma cell which can be differentiated into nerve cell and the nerve prosoma cell can be switched into inner ear hair cell and inner ear supporting cells, which provides experiment evidence for cochlear cell transplantation therapeutic sound perception nerve deafness and solves the problem of the transplantation cell resource.

Description

(1) Technical field [0001] The invention relates to a method for obtaining neural precursor cells by inducing bone marrow mesenchymal stem cells. (2) Background technology [0002] Bone marrow mesenchymal stem cells (mesenchymal stem cells, MSCs) not only participate in the microenvironment supporting hematopoiesis, but also have strong self-replication ability and multi-lineage differentiation potential. Different in vitro induction conditions can determine their differentiation direction, which may be the result of these induction Conditions turn on transcription factors that determine the direction of differentiation. When MSCs are transplanted into different tissues in the body through intravenous route or local injection, they can differentiate into such tissue cells in the corresponding tissues. The regeneration and transplantation of nerve cells after nervous system injury has puzzled people for a long time. Embryonic neural stem cell transplantation can promote the ...

Claims

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Application Information

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IPC IPC(8): C12N5/06C12N5/08C12N5/0775C12N5/0793
Inventor 徐娅苹管明雷云秋彭佳萍
Owner ZHEJIANG UNIV
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