Medical dressing with bioactivity and preparation method of medical dressing

A bioactive and growth factor technology, applied in the fields of biomedical engineering and materials science, can solve problems such as failure efficacy and cell wound deterioration.

Inactive Publication Date: 2016-11-23
南京天其美生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But some patents adopt the method of planting cells (especially stem cells) (CN201210585481; CN201210133427; CN201180012330; CN03134535; CN03134540; CN03134539; After transplantation to the wound, it will inev...

Method used

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  • Medical dressing with bioactivity and preparation method of medical dressing
  • Medical dressing with bioactivity and preparation method of medical dressing
  • Medical dressing with bioactivity and preparation method of medical dressing

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preparation example Construction

[0023] a. Preparation of the outer layer porous polymer material film: Take a part of the mass of the polymer material and dissolve it in 15 mL of chloroform solution, add six parts of the mass of salt particles with a particle size of 50-100 μm after the dissolution, and stir After uniformity, pour it into a pre-prepared mold and place it at room temperature for 24 hours; after the solvent evaporates, take the material out of the mold and place it in deionized water at 37°C for 3 hours on a shaker, take it out and dry it to obtain the outer porous polymer material layer;

[0024]b. Preparation of stem cell growth factor: use DMEM medium (complete medium) containing 10% fetal bovine serum, at 37°C, 5%CO 2 Stem cells were routinely cultured under certain conditions until the cell confluence reached about 90% and the cell concentration reached 1-10×10 6 , absorb the culture solution containing stem cell growth factors, centrifuge, discard the precipitate and take the supernatant...

Embodiment 1

[0031] a. Preparation of the outer layer porous polymer material film: Dissolve 2g of polylactic acid (PLA) in 15mL of chloroform solution, add 12g of salt particles with a particle size of 50μm after the dissolution, stir well and pour into the pre-prepared Spread the film into a film with a thickness of 1 mm in a mold, and place it at room temperature for 24 hours; after the solvent evaporates, take the material out of the mold and place it in deionized water at 37 ° C for 3 hours on a shaker, and then dry it to obtain the porous polymer material of the outer layer layer;

[0032] b. Preparation of stem cell growth factor: use DMEM medium (complete medium) containing 10% fetal bovine serum, at 37°C, 5%CO 2 Under certain conditions, the remaining embryonic stem cells derived from artificial insemination for IVF operation were routinely cultured until the cell confluence reached about 90% and the cell concentration reached 1×10 6 , absorb the culture solution containing stem ...

Embodiment 2

[0038] a, the preparation of the outer layer porous polymer material film: 15g polyglycolic acid (PGA) is dissolved in the chloroform solution of 15mL, after dissolving, add 90g of the salt particle that the particle diameter is 60 μm, stir and pour into pre- In the prepared mold, spread it into a film with a thickness of 0.5 mm, and place it at room temperature for 24 hours; after the solvent evaporates, take the material out of the mold and place it in deionized water at 37 ° C for 3 hours on a shaker, and then dry it to obtain the outer layer of porous polymer. Glycolic acid (PGA) layer;

[0039] b. Preparation of stem cell growth factor: use DMEM medium (complete medium) containing 10% fetal bovine serum, at 37°C, 5%CO 2 Embryonic stem cells derived from bovine artificial insemination were routinely cultured under certain conditions until the cell confluence reached about 90% and the cell concentration reached 2×10 6 , absorb the culture solution containing stem cell grow...

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Abstract

The invention relates to a medical dressing with bioactivity and a preparation method of the medical dressing and belongs to the technical field of biomedical engineering and material science. The medical dressing with the bioactivity is used for covering skin defect wounds caused by burning, scalding, operation, wounds and diseases and inducing wound repair and is prepared as follows: one or more of embryonic stem cells, umbilical cord blood stem cells, amniotic fluid stem cells, peripheral blood stem cells and bone mesenchymal stem cells of humans or animals are cultured in vitro, stem cell growth factors secreted in the logarithmic growth phase are collected and mixed in proportion with one or more of I type collagen, III type collagen, chitosan, hyaluronic acid, chondroitin sulfate and sodium alginate, thin-layer sponge is taken as an inner layer, a porous high-polymer material film outer layer prepared from a mixture formed by one or more of PLA (polylactic acid), PGA (polyglycolic acid), PLGA (poly(lactic-co-glycolic acid)) and PCL (polycaprolactone) is attached, the product is frozen-dried and cut, and the medical dressing with the bioactivity is formed and used for treating skin defect wounds caused by burning, scalding, operation, wounds and diseases and inducing wound repairing.

Description

technical field [0001] The invention relates to a biologically active medical dressing and a preparation method thereof, which are used for covering skin defect wounds caused by burns, scalds, operations, wounds and diseases, and inducing wound repair. It belongs to the field of biomedical engineering and material science and technology. Background technique [0002] The skin is composed of epidermis, dermis and subcutaneous tissue. Its function is to protect internal tissues from external damage; perform metabolism such as absorption, perspiration, secretion of sebum and excretion of waste; the skin can also regulate body temperature, feel pain, touch, pressure, Warm, cold and other stimuli, and has an immune effect. People will inevitably suffer from traumas of different severity in their life, especially trauma, natural disasters and surgical trauma after illness are often unavoidable. When the skin damage reaches a certain level, due to the fibroproliferative reaction,...

Claims

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Application Information

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IPC IPC(8): A61L15/32A61L15/28A61L15/26A61L15/44A61L15/42
Inventor 胡庆柳
Owner 南京天其美生物技术有限公司
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