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Cell injection for treating bone injury and preparation method thereof

A technology for bone injury and injection, which is applied in the field of injection for treating bone injury and its preparation, can solve the problems of unable to change the local microenvironment of the defect site, unable to fundamentally improve cartilage damage, and high surgical technical content requirements, so as to avoid immune rejection. , Reduced risk, simple and fast operation

Inactive Publication Date: 2011-11-09
董运海
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Non-drug treatment, drug treatment and surgical treatment methods can not fundamentally improve cartilage damage, drug treatment can only relieve the suffering of patients in a short period of time, and surgical treatment is not only expensive, but also unbearable for most patients. The treatment brings great trauma to the patients, and the surgical technology requires high content, which is difficult to implement in general hospitals due to insufficient conditions.
Autologous cartilage / osteoblast transplantation can repair cartilage damage to a certain extent, but because the sampled cartilage / osteoblast comes from the patient itself, it will inevitably cause certain damage to the original healthy parts, and at the same time, the bone damage The cause of the disease is not only cartilage / osteogenesis damage, but also related to the local microenvironment of the bone injury site, insufficient blood supply, etc.
Autologous cartilage / osteoblast implantation cannot change the local microenvironment of the defect site, and it is bound to fail to heal the damaged site fundamentally

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0010] Specific implementation mode 1: Extraction, isolation, cultivation and identification of bone marrow-derived mesenchymal stem cells

[0011] 1. Extraction, isolation and culture of bone marrow-derived mesenchymal stem cells

[0012] (1) Bone marrow extraction from the patient

[0013] Bone marrow was extracted by the method of bone marrow extraction from the anterior superior iliac spine. Routine disinfection, draping, and local anesthesia on one or both sides of the anterior superior iliac spine, puncture the bilateral anterior superior iliac spine with a bone puncture needle, and take bone marrow with a 10ml syringe in different directions.

[0014] (2) Extraction and preservation of nutritional factors in patients' blood

[0015] At the same time as the bone marrow is collected, a certain amount of blood is collected from the patient, and the nutrient components (platelets and plasma) are separated in the laboratory. register.

[0016] (3) Extraction and separati...

specific Embodiment approach 2

[0022] Specific embodiment two: Bone marrow mesenchymal stem cells culture method without exogenous animal serum

[0023] In order to effectively avoid contamination of cells by viruses that may exist in animal serum, in the process of cultivating bone marrow mesenchymal stem cells in the present invention, the additives in the medium used are isolated from the patient's blood to obtain effective nutrient components platelets and plasma , and mixed with low-sugar DMEM basal medium in a certain proportion to prepare a complete medium.

specific Embodiment approach 3

[0024] Specific implementation mode three: Safety detection of bone marrow mesenchymal stem cells and gel material composites

[0025] Collect bone marrow mesenchymal stem cells from the third to the fifth passage, according to 1×10 5 ~10 8 Each cell is mixed with 1-5ml biogel to form an injectable complex, in which a certain amount of blood-extracted growth factors are added. The survival rate, fungus, virus, and tumorigenicity of cells are detected according to relevant standards. The main reference standards are: "Chinese Pharmacopoeia" 2005 Edition Third Appendix XII A Page 73 and "Tissue Engineering Medical Products Part 12: Cells Guidelines for the processing of organs, tissues, and organs; regulations in the third appendix of the 2005 edition of the Chinese Pharmacopoeia; guidelines for the application of clinical trials for human somatic cell therapy, regulations for the management of experimental animals, etc. The results showed that the complex formed by the combin...

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Abstract

The invention relates to a cell injection for treating bone injury and a preparation method thereof, belonging to the field of tissue engineering. The injection is a compound formed by mesenchymal stem cells from autologous bone marrow, temperature sensitive gel and effective trophic factors separated and extracted from autoblood. The preparation method comprises the steps of: after extracting the bone marrow of a patient, separating and extracting the mesenchymal stem cells from the bone marrow, adding no animal serum in the amplification culture process, and mixing 1*(105-108) cells with 1-5ml of biological scaffold material to form the injectable compound. The cell injection for treating bone injury is mainly used for treating bone injuries including osteoarthritis, femoral head necrosis and delayed union and disunion of bone fracture, is convenient to use, has notable effect without immunological rejection, and can alleviate the great pain of patients caused by bone injury.

Description

technical field [0001] The invention relates to an injection for treating bone injury and a preparation method thereof, which is suitable for treating osteoarthritis, femoral head necrosis, delayed union or nonunion of fractures in orthopedics, and belongs to the field of tissue engineering. Background technique [0002] With the prolongation of life expectancy, the change of diet structure and the improvement of living standards in our country, there are more and more patients with bone injuries. At present, such as arthritis, femoral head necrosis, delayed union or nonunion of fracture are the most common, and the incidence rate is increasing every year. These diseases have brought a great impact on the lives of the people, but from the current treatment In terms of means, the effect is not very obvious. [0003] The first type of bone injury: arthritis. Arthritis is a common chronic disease. The most common types are osteoarthritis and rheumatoid arthritis. It is estimat...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P19/08
Inventor 董运海
Owner 董运海
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