Cartilage tissue engineering repair bracket and preparation method thereof

A cartilage tissue and engineering technology, applied in the field of cartilage tissue engineering repair scaffold and its preparation, can solve the problems of difficult retention of BMSCs, few BMSCs, etc., and achieve the effects of high mechanical strength and induction ability, promotion of adhesion, and long curative effect period.

Active Publication Date: 2014-06-25
PEKING UNIV THIRD HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the problem that there are few BMSCs in the cartilage defect area in the prior art and BMSCs are not easy to

Method used

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  • Cartilage tissue engineering repair bracket and preparation method thereof
  • Cartilage tissue engineering repair bracket and preparation method thereof
  • Cartilage tissue engineering repair bracket and preparation method thereof

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Embodiment 1

[0060] The embodiment of the present invention provides a cartilage tissue engineering repair scaffold, the components of the cartilage tissue engineering repair scaffold include natural polymer hydrogel and decalcified bone matrix, and the surface of the decalcified bone matrix is ​​coupled with bone marrow mesenchymal stem cells. and peptides.

[0061] The cartilage tissue engineering repair scaffold provided by the embodiment of the present invention includes a natural polymer hydrogel and a decalcified bone matrix coupled with a bone marrow mesenchymal stem cell affinity peptide on the surface. By combining natural polymer hydrogel with decalcified bone matrix, on the one hand, the cartilage tissue engineering repair scaffold has the plasticity, adhesion and injectability of hydrogel, making it have the natural biological scaffold material itself On the other hand, the decalcified bone matrix can provide a collagen fiber network framework, which endows the prepared cartila...

Embodiment 2

[0063] The embodiment of the present invention provides a cartilage tissue engineering repair scaffold, the components of the cartilage tissue engineering repair scaffold include natural polymer hydrogel and decalcified bone matrix, and the surface of the decalcified bone matrix is ​​coupled with bone marrow mesenchymal stem cells. and peptides.

[0064] Natural polymer hydrogel has high water content, plasticity and injectability, and is a commonly used material in tissue engineering research. The biggest advantage of natural polymer hydrogel is that it can maintain the spherical chondrocyte phenotype of the encapsulated cells and prevent dedifferentiation. It can be designed into a liquid form to make it injectable, and it can be better mixed with cartilage repair cells to avoid cell loss. It can be seen that the injectability and high adhesion of natural polymer hydrogel enable it to be widely used in the field of minimally invasive medicine. In addition, since the natura...

Embodiment 3

[0074] as attached figure 1 As shown, the embodiment of the present invention provides a preparation method of cartilage tissue engineering repair scaffold, comprising:

[0075] Step 101: Prepare demineralized bone matrix.

[0076] Step 102: coupling the bone marrow mesenchymal stem cell affinity peptide to the surface of the decalcified bone matrix to obtain the bone marrow mesenchymal stem cell affinity peptide modified demineralized bone matrix.

[0077] Step 103: Prepare natural polymer hydrogel solution.

[0078] Step 104: Inject the natural polymer hydrogel solution into the bone marrow mesenchymal stem cell affinity peptide-modified decalcified bone matrix, so that the natural polymer hydrogel solution completely penetrates into the bone marrow mesenchymal stem cell affinity peptide modified decalcified bone matrix, and undergo gelation reaction at a preset temperature to obtain a cartilage tissue engineering repair scaffold.

[0079] The preparation method of the ca...

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Abstract

The invention discloses a cartilage tissue engineering repair bracket and a preparation method thereof, and belongs to the cartilage tissue repair field. The bracket comprises natural high polymer water-gel and decalcified bone matrix which is coupled with affinitive peptides of mesenchymal stem cells on surface; the affinitive peptides of the mesenchymal stem cells are coupled on the surface of the decalcified bone matrix to obtain the cartilage tissue engineering repair bracket with special mesenchymal stem cell affinity. By being placed in a cartilage defective region, the cartilage tissue engineering repair bracket can collect a plurality of BMSC (bone marrow stromal cells); and in the bracket, under mating effect of the natural high polymer water-gel and the decalcified bone matrix, the BMSC can be retained in the cartilage defective region for a long time, so that the cartilage repair has a longer curative effect period. The invention further discloses a preparation method of the bracket, wherein the natural high polymer water-gel aqueous liquor is injected in the decalcified bone matrix which is coupled with affinitive peptides of mesenchymal stem cells on surface to carry out gelation reaction to obtain the bracket with higher mechanical strength and inducibility. The method is simple and high in practicability.

Description

technical field [0001] The invention relates to the field of cartilage repair, in particular to a cartilage tissue engineering repair bracket and a preparation method thereof. Background technique [0002] Cartilage tissue engineering is a technology that uses tissue engineering methods to construct hyaline cartilage tissue. The three basic elements in cartilage tissue engineering are: seed cells, degradable cartilage tissue engineering repair scaffolds and cell growth regulators. For cartilage tissue engineering repair scaffolds, because cartilage tissue engineering repair scaffolds can provide a three-dimensional space structure for the construction of cartilage repair cells, which is conducive to cell adhesion and proliferation, and provides a good growth environment for cell growth, usually cartilage tissue engineering repair The scaffold is placed in the cartilage defect area for filling and repairing the cartilage defect to promote the regeneration and repair of carti...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/52A61L27/36A61L27/34A61L27/56
Inventor 敖英芳黄洪杰张辛邵振兴陈海峰周春燕
Owner PEKING UNIV THIRD HOSPITAL
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